Choline catabolism, σ54 factor and NtrC are required for the full expression of the Pseudomonas aeruginosa phosphorylcholine phosphatase gene

Microbiological Research

Volumn 166, Issue 5, 2011, Pages 380-390

  Massimelli, Maria J ^a   Sánchez, Diego G ^a   Buchieri, Maria V ^a   Olvera, Leticia ^b   Beassoni, Paola R ^a   Schweizer, Herbert P ^c   Morett, Enrique ^b   Lisa, Angela T ^a  

^a DEPARTAMENTO DE QUÍMICA   (Argentina)

^b INSTITUTO DE BIOTECNOLOGÍA   (Mexico)

^c Department of Environmental and Radiological Health Sciences   (United States)

Author keywords

Choline; Phosphorylcholine phosphatase; Pseudomonas aeruginosa; Regulation

Indexed keywords

CHOLINE; CONSENSUS SEQUENCE; DELETION ANALYSIS; ENHANCER BINDING PROTEINS; GENE CODING; INTEGRATION HOST FACTOR; PALINDROMIC; PHOSPHOLIPASE C; PHOSPHORYLCHOLINE; PROMOTER ELEMENTS; PSEUDOMONAS AERUGINOSA; REGULATION; REGULATORY PROTEIN;

BACTERIA; METABOLISM; PHOSPHATASES;

GENE EXPRESSION;

CHOLINE; PHOSPHATASE; PHOSPHORYLCHOLINE; SIGMA FACTOR RPON; TRANSCRIPTION FACTOR;

AMMONIUM COMPOUND; BACTERIUM; CATABOLISM; ENZYME ACTIVITY; EXPERIMENTAL STUDY; GENE EXPRESSION; PATHOGEN; PROTEIN;

ARTICLE; BACTERIAL GENE; GENE DELETION; GENE EXPRESSION; GENE EXPRESSION REGULATION; GENE ORDER; GENETICS; METABOLISM; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; PROMOTER REGION; PSEUDOMONAS AERUGINOSA;

BASE SEQUENCE; CHOLINE; GENE EXPRESSION; GENE EXPRESSION REGULATION, BACTERIAL; GENE ORDER; GENES, BACTERIAL; MOLECULAR SEQUENCE DATA; PHOSPHORIC MONOESTER HYDROLASES; PHOSPHORYLCHOLINE; PROMOTER REGIONS, GENETIC; PSEUDOMONAS AERUGINOSA; RNA POLYMERASE SIGMA 54; SEQUENCE DELETION; TRANSCRIPTION FACTORS;

PSEUDOMONAS AERUGINOSA;

EID: 79959317824     PISSN: 09445013     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.micres.2010.07.004     Document Type: Article

References (37)

1. Barrios H., Valderrama B., Morett E. Compilation and analysis of σ54-dependent promoter sequences. Nucleic Acids Res 1999, 27:4305-4313.
2. Beassoni P.R., Berti F.P., Otero L.H., Risso V.A., Ferreyra R.G., Lisa A.T., et al. Preparation and biophysical characterization of recombinant phosphorylcholine phosphatase of Pseudomonas aeruginosa. Protein Expr Purif 2010, 71:153-159.
3. Beassoni P.R., Otero L.H., Massimelli M.J., Lisa A.T., Domenech C.E. Critical active-site residues identified by site-directed mutagenesis in Pseudomonas aeruginosa phosphorylcholine phosphatase, a new member of the haloacid dehalogenases hydrolase superfamily. Curr Microbiol 2006, 53:534-539.
4. Beassoni P.R., Otero L.H., Lisa A.T., Domenech C.E. Using a molecular model and kinetic experiments in the presence of divalent cations to study the active site and catalysis of Pseudomonas aeruginosa phosphorylcholine phosphatase. Biochim Biophys Acta 2008, 1784:2038-2044.
5. Buck M., Gallegos M.-T., Studholme D.J., Guo Y., Gralla D.J. The bacterial enhancer-dependent σ54 (σN) transcription factor. J Bacteriol 2000, 182:4129-4136.
6. Cannon W., Missailidis S., Smith C., Cottier A., Austin S., Moore M., et al. Core RNA polymerase and promoter DNA interactions of purified domains of sN: bipartite functions. J Mol Biol 1995, 248:781-783.
7. Choi K.H., Schweizer H.P. An improved method for rapid generation of unmarked Pseudomonas aeruginosa deletion mutants. BMC Microbiol 2005, 5:30.
8. Choi K.H., Gaynor J.B., White K.G., López C., Bosio C.M., Karkhoff-Schweizer R.R., et al. A Tn7-based broad-range bacterial cloning and expression system. Nat Methods 2005, 2:443-448.
9. Choi K.H., Kumar A., Schweizer H.P. A 10-min method for preparation of highly electrocompetent Pseudomonas aeruginosa cells: application for DNA fragment transfer between chromosomes and plasmid transformation. J Microbiol Methods 2006, 64:391-397.
10. Ciria R., Abreu-Goodger C., Morett E., Merino E. GeConT: gene context analysis. Bioinformatics 2004, 20:2307-2308.
11. Hervas A.B., Canosa I., Santero E. Transcriptome analysis of Pseudomonas putida in response to nitrogen availability. J Bacteriol 2008, 191:416-420.
12. Heurlier K., Deónervaud V., Pessi G., Reimmann C., Haas D. Negative control of quorum sensing by RpoN (σ54) in Pseudomonas aeruginosa PAO1. J Bacteriol 2003, 185:2227-2235.
13. Itoh Y., Nishijyo T., Nakada Y. Histidine catabolism and catabolite regulation. Pseudomonas 2007, 371-395. Springer, Dordrecht, The Netherlands. J.L. Ramos, A. Filloux (Eds.).
14. Kustu S., North A.K., Weiss D.S. Prokaryotic transcriptional enhancers and enhancer-binding proteins. Trends Biochem Sci 1991, 16:397-402.
15. Li W., Lu C.-D. Regulation of carbon and nitrogen utilization by CbrAB and NtrBC two component systems in Pseudomonas aeruginosa. J Bacteriol 2007, 189:5413-5420.
16. Lisa A.T., Garrido M.N., Domenech C.E. Induction of acid phosphatase and cholinesterase in P. aeruginosa and their in vitro control by choline, acetylcholine and betaine. Mol Cell Biochem 1983, 50:149-155.
17. Lisa A.T., Lucchesi G.I., Domenech C.E. Pathogenicity of Pseudomonas aeruginosa and its relationship to the choline metabolism through the action of cholinesterase, acid phosphatase and phospholipase C. Curr Microbiol 1994, 29:193-199.
18. Lisa A.T., Beassoni P.R., Masssimelli M.J., Otero L.H., Domenech C.E. A glance on Pseudomonas aeruginosa phosphorylcholine phosphatase, an enzyme whose synthesis depends on the presence of choline in its environment. Communicating current research and educational topics and trends in applied microbiology 2007, vol. I:255-262. Formatex Press, Badajoz, Spain.
19. Lucchesi G.I., Lisa A.T., Casale C.H., Domenech C.E. Carnitine resembles choline in the induction of cholinesterase, acid phosphatase, and phospholipase C and in its action as an osmoprotectant in Pseudomonas aeruginosa. Curr Microbiol 1995, 28:71-76.
20. Massimelli M.J., Beassoni P.R., Forrellad M.A., Barra J.L., Garrido M.N., Domenech C.E., Lisa A.T. Identification, cloning and expression of Pseudomonas aeruginosa phosphorylcholine phosphatase gene. Curr Microbiol 2005, 50:251-256.
21. Mendoza A., Olvera L., Olvera M., Grande R., Jiménez-Jacinto V., Taboada B., et al. Genome-wide identification of transcription start sites, promoters and transcription factor binding sites in E. coli. PLoS One 2009, 4:e7526.
22. Miller JH Experiments in molecular genetics 1972, Cold Spring Harbor Laboratory Press, New York.
23. Morett E., Buck M. In vivo studies on the interaction of RNA polymerase-sigma 54 with the Klebsiella pneumoniae and Rhizobium meliloti nifH promoters. The role of NifA in the formation of an open promoter complex. J Mol Biol 1989, 210:65-77.
24. Morett E., Segovia I. The σ54 bacterial enhancer-binding protein family: mechanism of action and phylogenetic relationship of their functional domains. J Bacteriol 1993, 175:6067-6074.
25. Ninfa A.J., Atkinson M.R., Kamberon E.S., Feng J., Ninfa EG Control of nitrogen assimilation by the NRI-NRII two-component system of enteric bacteria. Two-component signal transduction 1995, 67-88. ASM Press, Washington, DC. J.A. Hoch, T.J. Silhavy (Eds.).
26. Nishijyo T., Haas D., Itoh Y. The CbrA-CbrB two-component regulatory system controls the utilization of multiple carbon and nitrogen sources in Pseudomonas aeruginosa. Mol Microbiol 2001, 40:917-931.
27. Ochsner U.A., Vasil A.I., Johnson Z., Vasil M.L. Pseudomonas aeruginosa fur overlaps with a gene encoding a novel outer membrane lipoprotein, OmlA. J Bacteriol 1999, 181:1099-1109.
28. Olvera L., Mendoza-Vargas A., Flores N., Olvera M., Sigala J.C., Gosset G., et al. Transcription analysis of central metabolism genes in Escherichia coli. Possible roles of sigma 38 in their expression, as a response to carbon limitation. PLoS One 2009, 4:e7466.
29. Otero L.H., Beassoni P.R., Lisa A.T., Domenech C.E. Transition from octahedral to tetrahedral geometry causes the activation or inhibition by Zn2+ of Pseudomonas aeruginosa phosphorylcholine phosphatase. Biometals 2010, 23:307-3014.
30. Popham D.L., Szeto D., Keener J., Kustu S. Function of a bacterial activator protein that binds to transcriptional enhancers. Science 1989, 243:629-635.
31. Sambrook J., Russel D.W. Molecular cloning: a laboratory manual 2001, Cold Spring Harbor Laboratory Press, New York.
32. Schweizer H.P. A method for construction of bacterial hosts for lac-based cloning and expression vectors: alpha-complementation and regulated expression. Biotechniques 1994, 17:452-456.
33. Studholme D.J., Dixon R. Domain architectures of sigma 54-dependent transcriptional activators. J Bacteriol 2003, 185:1757-1767.
34. Wargo M.J., Szwergold B.S., Hogan D.A. Identification of two gene clusters and a transcriptional regulator required for Pseudomonas aeruginosa glycine betaine catabolism. J Bacteriol 2008, 190:2690-2699.
35. Wargo M.J., Ho T.C., Gross M.J., Whittaker L.A., Hogan D.A. GbdR regulates Pseudomonas aeruginosa plcH and pchP transcription in response to choline catabolites. J Bacteriol 2009, 77:1103-1111.
36. Wozniak D.J. Integration host factor and sequences downstream of the Pseudomonas aeruginosa algD transcription start site are required for expression. J Bacteriol 1994, 176:5068-5076.
37. Zhang X.-X., Rainey P.B. Dual involvement of CbrAB and NtrBC in the regulation of histidine utilization in Pseudomonas fluorescens SBW25. Genetics 2008, 178:185-195.