Identification, cloning, and expression of Pseudomonas aeruginosa phosphorylcholine phosphatase gene

Current Microbiology

Volumn 50, Issue 5, 2005, Pages 251-256

  Massimelli, María J ^a   Beassoni, Paola R ^a   Forrellad, Marina A ^a   Barra, José L ^b   Garrido, Mónica N ^a   Domenech, Carlos E ^a   Lisa, Angela T ^a  

^a DEPARTAMENTO DE QUÍMICA   (Argentina)

^b UNIVERSIDAD NACIONAL DE CÓRDOBA   (Argentina)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL ENZYME; PHOSPHATE; PHOSPHOETHANOLAMINE; PHOSPHORYLCHOLINE; PHOSPHORYLCHOLINE PHOSPHATASE; RECOMBINANT ENZYME; UNCLASSIFIED DRUG;

ARTICLE; BACTERIAL GENE; BACTERIAL GENOME; BACTERIAL STRAIN; BINDING AFFINITY; BINDING SITE; CATALYSIS; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME INHIBITION; ENZYME PURIFICATION; ENZYME SYNTHESIS; ESCHERICHIA COLI; GENE EXPRESSION; GENE IDENTIFICATION; HYDROLYSIS; INSTRUMENTATION; MOLECULAR CLONING; MOLECULAR WEIGHT; MUTAGENESIS; MUTANT; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN EXPRESSION; PSEUDOMONAS AERUGINOSA;

BASE SEQUENCE; CLONING, MOLECULAR; ESCHERICHIA COLI; ETHANOLAMINES; MOLECULAR SEQUENCE DATA; NITROPHENOLS; ORGANOPHOSPHORUS COMPOUNDS; PHOSPHORIC MONOESTER HYDROLASES; PHOSPHORYLCHOLINE; PLASMIDS; PSEUDOMONAS AERUGINOSA;

PSEUDOMONAS AERUGINOSA;

EID: 21044436304     PISSN: 03438651     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00284-004-4499-9     Document Type: Article

References (26)

1. E Beck G Ludwig E Auerswald B Reiss E Schaller 1982 Nucleotide sequence and exact localization of the neomycin phosphotransferase gene from transposon Tn5 Gene 19 327 336
2. M Bradford 1976 A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding Anal Biochem 72 248 254
3. JF Collet V Stroobant M Pirard G Delpierre E Van Schaftingen 1998 A new class of phosphotransferases phosphorylated on an aspartate residue in an amino-terminal DXDX(T/V) motif J Biol Chem 273 14107 14112
4. CN Cronin WS McIntire 1999 pUCP-Nco and pUCP-Nde: Escherichia - Pseudomonas shuttle vectors for recombinant protein expression in Pseudomonas Anal Biochem 272 112 115
5. C Fiske Y Subarrow 1925 The colorimetric determination of phosphorus J Biol Chem 66 361 375
6. JL Garcia AR Sanchez-Beato FJ Medrano E Lopez 1998 Versatility of choline binding domain Microb Drug Resist 4 25 36
7. D Garfin 1990 One-dimensional gel electrophoresis Methods Enzymol 182 425 441
8. MN Garrido AT Lisa CE Domenech 1988 Pseudomonas aeruginosa acid phosphatase contains an anionic site with a trimethyl subsite. Kinetic evidences obtained with alkylammonium ions Mol Cell Biochem 84 41 49
9. C-L Ho M Noji K Saito 1999 Plastidic pathway of serine biosynthesis molecular cloning and expression of 3-phosphoserine phosphatase from Arabidopsis thaliana J Biol Chem 274 11007 11012
10. EV Koonin R L Tatusov 1994 Computer analysis of bacterial haloacid dehalogenases defines a large superfamily of hydrolases with diverse specificity. Application of an iterative approach to database search J Mol Biol 244 125 132
11. ME Kovach RW Phillips PH Elzer RM Roop KM Peterson 1994 pBBR1MCS: a broad-host-range cloning vector Biotechniques 16 800 802
12. TA Lisa CH Casale CE Domenech 1994 Cholinesterase, acid phosphatase and phospholipase C of Pseudomonas aeruginosa under hyperosmotic conditions in a high-phosphate medium Curr Microbiol 28 71 76
13. TA Lisa GI Lucchesi CE Domenech 1994 Pathogenicity of Pseudomonas aeruginosa and its relationship to the choline metabolism through the action of cholinesterase, acid phosphatase and phospholipase C Curr Microbiol 29 193 199
14. GI Lucchesi TA Lisa CH Casale CE Domenech 1995 Carnitine resembles choline in the induction of cholinesterase, acid phosphatase, and phospholipase C and in its action as an osmoprotectant in Pseudomonas aeruginosa Curr Microbiol 30 55 60
15. GI Lucchesi AT Lisa CE Domenech 1989 Pseudomonas aeruginosa phospholipase C activity in a high phosphate medium FEMS Microbiol Lett 57 335 338
16. H Nielsen S Brunak G von Heijne 1999 Machine learning approaches for the prediction of signal peptides and other protein sorting signals Protein Eng 12 3 9
17. RM Ostroff AI Vasil ML Vasil 1990 Molecular comparison of a nonhemolytic and a hemolytic phospholipase C from Pseudomonas aeruginosa J Bacteriol 172 5915 5923
18. M Rella A Mercenier D Haas 1985 Transposon insertion mutagenesis of P. aeruginosa with a Tn5 derivative: application to physical mapping of the arc gene cluster Gene 33 293 303
19. MA Salvano CE Domenech 1999 Kinetic properties of purified Pseudomonas aeruginosa phosphorylcholine phosphatase indicated that this enzyme may be utilized by bacteria to colonize in different environments Curr Microbiol 39 1 8
20. J Sambrook DW Russel 2001 Molecular cloning: a laboratory manual 3 Cold Spring Harbor Laboratory Press Cold Spring Harbor, NY
21. AL Serra JF Mariscotti JL Barra GI Lucchesi CE Domenech AT Lisa 2002 Glycine betaine transmethylase mutant of Pseudomonas aeruginosa J Bacteriol 184 4301 4303
22. R Simon U Priefer A Pühler 1983 A broad host range mobilization system for in vitro genetic engineering: transposon mutagenesis in Gram negative bacteria Bio/Technology 1 784 790
23. MJ Stonehouse A Cota-Gomez SK Parker WE Martin JA Hankin RC Murphy W Chen KB Lim M Hackett AI Vasil ML Vasil 2002 A novel class of microbial phosphocholine-specific phospholipase C Mol Microbiol 46 661 676
24. CK Stover XQ Pham AL Erwin SD Mizoguchi P Warrener MJ Hickey 2000 Complete genome sequence of Pseudomonas aeruginosa PA01, an opportunistic pathogen Nature 406 959 964
25. ML Vasil RW Prince VD Shortridge 1993 Exoproducts: Pseudomonas exotoxin A and phospholipase C RB Fick Jr Pseudomonas aeruginosa the opportunist: pathogenesis and disease CRC Press Boca Raton, FL 60 77
26. ML Vasil AI Vasil VD Shortridge 1994 Phosphate and osmoprotectants in the pathogenesis of Pseudomonas aeruginosa A Torriani-Gorrini E Yagil S Silver Phosphate in microorganisms: cellular and molecular biology ASM Press Washington, DC 126 132