Development of hybrid human interferon alfa-2 strain-producers and the use of enteropeptidase for production of N-terminal methionine-free interferons

Molecular Biology

Volumn 45, Issue 3, 2011, Pages 466-471

  Shirokov, D A ^a   Ryabichenko, V V ^a   Akishina, R I ^a   Ospelnikova, T P ^b   Glazunov, A V ^a   Chestukhina, G G ^a   Veiko, V P ^a  

^a STATE RESEARCH INSTITUTE OF GENETICS AND SELECTION OF INDUSTRIAL MICROORGANISMS   (Russian Federation)

^b GAMALEYA RESEARCH INSTITUTE OF EPIDEMIOLOGY AND MICROBIOLOGY   (Russian Federation)

Author keywords

enterokinase; enteropeptidase; interferon 2a; interferon 2b

Indexed keywords

ESCHERICHIA COLI;

EID: 79958864919     PISSN: 00268933     EISSN: 16083245     Source Type: Journal    
DOI: 10.1134/S0026893311020178     Document Type: Article

References (16)

1. Khaitov R. M., Ignat'eva G. A., Sidorovich I. G. 2000. Immunologiya (Immunology). Moscow: Meditsina, pp. 193-195.
2. Pestka S., Krause C. D., Walter M. R. 2004. Interferons, interferon-like cytokines, and their receptors. Immunol. Rev. 202, 8-32.
3. Masci P., Bukowski R. M., Patten P. A., Osborn B. L., Borden E. C. 2003. New and modified interferon alfas: Preclinical and clinical data. Curr. Oncol. Rep. 5, 108-113.
4. Baron E., Narula S. 1990. From cloning to a commercial realization: Human alpha interferon. Crit. Rev. Biotechnol. 10, 179-190.
5. Meinnel T., Mechulam Y., Blanquet S. 1993. Methionine as translation start signal: A review of the enzymes of the pathway in Escherichia coli. Biochimie. 75, 1061-1075.
6. Mikhailova A. G., Shibanova E. D., Rumsh L. D., Antonov V. K. 1994. Enteropeptidase and its use for cleavage of chimeric proteins. Bioorg. Khim. 20, 883-893.
7. Mann N. S., Mann S. K. 1994. Enterokinase. Proc. Soc. Exp. Biol. Med. 206, 114-118.
8. Li M., Huang D. 2007. Purification and characterization of prokaryotically expressed human interferon-α2. Biotechnol. Lett. 29, 1025-1029.
9. Semikhin A. S., Lyashchuk A. M., Mezentseva M. V., Tregubova M. I., Sergienko O. V., Poletaeva N. N., Naroditskiy B. S., Karyagina A. S., Lunin V. G., Gintsburg A. L. 2009. Human recombinant interferon-β constructed on the basis of affinity-binding domain technology. Mol. Genet. Mikrobiol. Virusol. 4, 38-41.
10. Mogutov M. A., Yarotskiy S. V., Skrypin V. I., Yakovenko A. R., Chezhgalova N. A., Selishchev S. V. RF Patent no. RU 2319502C1.
11. Shekhter I. I., Veiko V. R., Bulenkov M. T., Ratmanova K. I., Debabov V. G. 1991. Mutant forms of human interferon α2 obtained by site-directed mutagenesis with the uracilrerair system. Mol. Biol. (Moscow). 25, 129-136.
12. Sambrook J., Fritsch E. F., Maniatis T. 1989. Molecular Cloning: A Laboratory Manual. Cold Spring Harbor, NY: Cold Spring Harbor Lab. Press.
13. Laemmli U. K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 227, 680-685.
14. Ershov F. I., Tazulakhova E. B., Narovlyanskii A. N., Grigoryan S. S., Mezentseva M. V., Shcherbenko V. E. 2005. Methodological guidelines for analyzing specific activity of interferon inducers. In: Rukovodstvo po eksperimental'nomu (doklinicheskomu) izucheniyu farmakologicheskikh veshchestv (A Manual on Experimental (Preclinical) Analysis of Pharmacological Substances). Khabriev R. U., Ed. Moscow: Medicine, pp. 558-571.
15. Pan S. H., Malcolm B. A. 2000. Reduced background expression and improved plasmid stability with pET vectors in BL21 (DE3). BioTechniques. 29, 1234-1238.
16. Grossman T. H., Kawasaki E. S., Punreddy S. R., Osburne M. S. 1998. Spontaneous cAMP-dependent derepression of gene expression in stationary phase plays a role in recombinant expression instability. Gene. 209, 95-103.