Multi-parameter flow cytometry and cell sorting reveal extensive physiological heterogeneity in Bacillus cereus batch cultures

Biotechnology Letters

Volumn 33, Issue 7, 2011, Pages 1395-1405

  Want, Andrew ^a   Hancocks, Helen ^b   Thomas, Colin R ^b   Stocks, Stuart M ^c   Nebe von Caron, Gerhard ^d   Hewitt, Christopher J ^a  

^a LOUGHBOROUGH UNIVERSITY   (United Kingdom)

^b UNIVERSITY OF BIRMINGHAM   (United Kingdom)

^c NOVOZYMES A S   (Denmark)

^d Inverness Medical International   (United Kingdom)

Author keywords

3,3 Dihexylocarbocyanine iodide; Bacillus cereus; Bis (1,3 dibutylbarbituric acid) trimethine oxonol; Cell sorting; Flow cytometry; Membrane integrity; Membrane potential

Indexed keywords

AGAR PLATES; BACTERIAL CELLS; BATCH CULTURE; BIS-(1,3-DIBUTYLBARBITURIC ACID) TRIMETHINE OXONOL; CELL SORTING; COLONY SIZE; DYNAMIC POPULATION; GROWTH CURVES; LAG PHASIS; MEMBRANE INTEGRITY; MEMBRANE POTENTIAL; MULTI-PARAMETER FLOW CYTOMETRY; PHYSIOLOGICAL STATE; REDUCTASE ACTIVITY; SINGLE CELLS;

BACILLI; BACTERIOLOGY; BATCH CELL CULTURE; CELLS; FLOW CYTOMETRY; PHYSIOLOGICAL MODELS; PHYSIOLOGY;

BACILLUS CEREUS;

FLUORESCENT DYE;

ARTICLE; BACILLUS CEREUS; CLASSIFICATION; FLOW CYTOMETRY; GENETIC VARIABILITY; GROWTH, DEVELOPMENT AND AGING; METABOLISM; METHODOLOGY; STAINING;

BACILLUS CEREUS; FLOW CYTOMETRY; FLUORESCENT DYES; GENETIC VARIATION; STAINING AND LABELING;

BACILLUS CEREUS; BACTERIA (MICROORGANISMS);

EID: 79958766577     PISSN: 01415492     EISSN: 15736776     Source Type: Journal    
DOI: 10.1007/s10529-011-0566-z     Document Type: Article

References (27)

1. Amanullah A, Hewitt CJ, Nienow AW, Lee C, Chartrain M, Buckland B, Drew S, Woodley J (2002) Measurement of strain dependent toxicity in the indene bioconversion using multi-parameter flow cytometry. Biotechnol Bioeng 80: 239-249.
2. Boswell CD, Hewitt CJ, Mackaskie LE (1998) An application of bacterial flow cytometry: evaluation of the toxic effects of four heavy metals on Acinetobacter sp. with potential for bioremediation of contaminated wastewaters. Biotechnol Lett 20: 857-863.
3. Chamsartra S, Hewitt CJ, Nienow AW (2005) The impact of fluid mechanical stress on Corynebacterium glutamicum during continuous cultivation in an agitated bioreactor. Biotechnol Lett 27: 693-700.
4. Davey HM, Kell DB (1996) Flow cytometry and cell sorting of heterogeneous microbial populations: the importance of single-cell analyses. Microbiol Rev 60: 641-696.
5. Haeusser DP, Levin PA (2008) The great divide: coordinating cell cycle events during bacterial growth and division. Curr Opin Microbiol 11: 94-99.
6. Haugland RP (2002) Handbook of fluorescent probes and research chemicals. Molecular Probes, Inc., Eugene, OR.
7. Hewitt CJ, Nebe-von-Caron G (2001) An industrial application of multi-parameter flow cytometry: assessment of cell physiological state and its application to the study of microbial fermentations. Cytometry 44: 179-187.
8. Hewitt CJ, Nebe-von-Caron G (2004) The application of multi-parameter flow cytometry to monitor individual microbial cell physiological state. Adv Biochem Eng Biotechnol 89: 197-223.
9. Hewitt CJ, Nienow AW (2007) The scale-up of microbial batch and fed-batch fermentation processes. Adv Appl Microbiol 62: 105-135.
10. Hewitt CJ, Boon LA, McFarlane CM, Nienow AW (1998) The use of flow cytometry to study the impact of fluid mechanical stress on E. coli during continuous cultivation in an agitated bioreactor. Biotechnol Bioeng 59: 612-620.
11. Hewitt CJ, Nebe-von-Caron G, Nienow AW, McFarlane CM (1999) The use of multi-parameter flow cytometry to compare the physiological response of Escherichia coli W3110 to glucose limitation during batch, fed-batch and continuous culture cultivation. J Biotechnol 75: 251-254.
12. Jepras RI, Paul FE, Pearson SC, Wilkinson MJ (1997) Rapid assessment of antibiotic effects on E. coli by bis-(1, 3-dibutylbarbituric acid) trimethine oxonol and flow cytometry. Antimicrob Ag Chemother 41: 2001-2005.
13. Lewis G, Taylor IW, Nienow AW, Hewitt CJ (2004) The application of multi-parameter flow cytometry to the study of recombinant Escherichia coli batch fermentation processes. J Ind Microbiol Biotechnol 31: 311-322.
14. Lopes da Silva T, Reis A, Kent CA, Kosseva M, Roseiro JC, Hewitt CJ (2005) Stress-induced physiological responses to glucose and lactose pulses in Bacillus licheniformis continuous culture fermentation processes as measured by multi-parameter flow cytometry. Biochem Eng J 24: 31-41.
15. Monfort P, Baleux B (1996) Cell cycle characteristics and changes in membrane potential during growth of Escherichia coli as determined by cyanine fluorescent dye and flow cytometry. J Microbiol Methods 25: 79-86.
16. Müller S, Loffhagen N, Bley T, Babel W (1996) Membrane-potential-related fluorescence intensity indicates membrane injury. Microbiol Res 151: 127-131.
17. Nebe-von-Caron G, Badley RA (1995) Viability assessment of bacteria in mixed populations using flow-cytometry. J Microsc 179: 55-66.
18. Nebe-von-Caron G, Mueller S (2007) Bugs in the Beam. In: (ed) JP Robinson, MMK Holdings, Cytometry vol 10, ISBN 978-1-890473-10-5.
19. Nebe-von-Caron G, Stephens PJ, Hewitt CJ, Powell JR, Badley RA (2000) Analysis of bacterial function by multi-colour and single cell sorting. J Microbiol Methods 42: 97-114.
20. Oliver JD (2010) Recent findings on the viable but non-culturable state in pathogenic bacteria. FEMS Microbiol Rev 34: 414-425.
21. Reis A, Lopes da Silva T, Kent CA, Kosseva M, Roseiro JC, Hewitt CJ (2005) The use of multi-parameter flow cytometry to study the impact of limiting substrate, agitation intensity and dilution rate on Bacillus licheniformis CCMI 1034 aerobic continuous culture fermentations. Biotechnol Bioeng 92: 568-578.
22. Rezaeinejada S, Ivanov V (2010) Heterogeneity of Escherichia coli population by respiratory activity and membrane potential of cells during growth and long-term starvation. J Micres doi: 10. 1016/j. micres. 2010. 01. 007.
23. Sebastine IM, Stocks SM, Cox PW, Thomas CR (1999) Characterisation of percentage viability of Streptomyces clavuligerus using image analysis. Biotechnol Technol 13: 419-423.
24. Shapiro HM (2003) Practical flow cytometry. Alan R. Liss Inc, New York.
25. Shi L, Günther S, Hübschmann T, Wick LY, Harms H, Müller S (2007) Limits of propidium iodide as a cell viability indicator for environmental bacteria. Cytometry 71: 592-598.
26. Stocks SM, Thomas CR (2001) Viability, strength, and fragmentation of Accharopolyspora erythraea in submerged fermentation. Biotechnol Bioeng 75: 702-709.
27. Want A, Thomas ORT, Kara B, Liddell J, Hewitt CJ (2009) Studies related to the production of antibody fragments (Fabs) in Escherichia coli W3110 fed-batch fermentation processes using multi-parameter flow cytometry. Cytometry 75: 148-154.