Application of multi-parameter flow cytometry using fluorescent probes to study substrate toxicity in the indene bioconversion

Biotechnology and Bioengineering

Volumn 80, Issue 3, 2002, Pages 239-249

  Amanullah, A ^a   Hewitt, C J ^b   Nienow, A W ^b   Lee, C ^c   Chartrain, M ^c   Buckland, B C ^c   Drew, S W ^c   Woodley, J M ^a  

^a UNIVERSITY COLLEGE LONDON   (United Kingdom)

^b UNIVERSITY OF BIRMINGHAM   (United Kingdom)

^c MERCK RESEARCH LABORATORIES   (United States)

Author keywords

Indene bioconversion; Indene toxicity; Multi parameter flow cytometry; Rhodococcus

Indexed keywords

BACTERIA; BIOLOGICAL MEMBRANES; BIOMASS; CYTOLOGY; ENZYME INHIBITION; FLUORESCENCE; PHYSIOLOGY; POLARIZATION; TOXICITY;

CYTOMETRY;

BIOCONVERSION;

FLUORESCENT DYE; INDENE DERIVATIVE; INDINAVIR; PROTEINASE INHIBITOR;

ARTICLE; BACTERIAL CELL; BIOMASS; BIOTRANSFORMATION; CELL MEMBRANE; CELL RESPIRATION; FLOW CYTOMETRY; LIGHT SCATTERING; MEMBRANE DEPOLARIZATION; MEMBRANE POTENTIAL; METABOLISM; OPTICAL DENSITY; OXYGEN CONSUMPTION; RHODOCOCCUS; SYNTHESIS; TOXICITY;

ADENOSINE TRIPHOSPHATE; BIOREACTORS; BIOTRANSFORMATION; CATALYSIS; CELL MEMBRANE; FERMENTATION; FLOW CYTOMETRY; FLUORESCENT DYES; INDENES; OXYGEN; REFERENCE VALUES; REPRODUCIBILITY OF RESULTS; RHODOCOCCUS; SENSITIVITY AND SPECIFICITY; STAINING AND LABELING;

BACTERIA (MICROORGANISMS); RHODOCOCCUS;

EID: 0037027383     PISSN: 00063592     EISSN: None     Source Type: Journal    
DOI: 10.1002/bit.10477     Document Type: Article

References (30)

1. Allman R, Manchee R, Lloyd D. 1993. Flow cytometric analysis of heterogeneous bacterial populations. In: Lloyd D, editor. Flow Cytometry in Microbiology. London: Springer-Verlag. p 27-47.
2. Bennik MHJ, Verhuel A, Abee T, Naaktgeboren-Stoffels G, Gorris GM, Smid FI. 1997. Interactions of nisin and pediocin PA-1 with closely related lactic acid bacteria that manifest over 100-fold differences in bacteriocin sensitivity. Appl Environ Microbiol 63:3628-3636.
3. Boswell CD, Hewitt CJ, Macaskie LE. 1998. An application of bacterial flow cytometry: Evaluation of the toxic effects of four heavy metals on Acinetobacter sp. with potential for bioremediation of contaminated wastewaters. Biotech Lett 20:857-863.
4. Boucabeille C, Letellier L, Simonet J, Henckes G. 1998. Mode of action of linenscin OC2 against Listeria innocua. Appl Environ Microbiol 64:3416-3421.
5. Buckland BC, Drew SW, Connors NC, Chartrain MC, Lee C, Salmon PM et al. 1999. Microbial conversion of indeno to indandiol: A key intermediate in the synthesis of CRIXIVAN. J Metabol Eng 1:63-74.
6. Chartrain M, Jackey B, Taylor C, Sandford V, Gbewonyo K, Lister L et al. 1998. Bioconversion of indene to cis (IS,2R) indandiol and trans (IR,2R) indandiol by Rhodococcus species. J Ferment Bioeng 86:550-558.
7. Connors N, Prevoznak R, Chartrain M, Reddy R, Singvi Patel Z, Olewinski R et al. 1997. Conversion of indene to cis-(1S),(2R)-indandiol by mutants of Pseudomonas putida F1. J Ind Microbiol Biotechnol 18:353-359.
8. Davey HM, Kell DB. 1996. Flow cytometry and cell sorting of heterogenous microbial populations: The importance of single cell analyses. Microbiol Rev 60:641-696.
9. Fouchet P, Jayat C, Hechaed Y. 1993. Recent advances of flow cytometry in fundamental and applied microbiology. Biol Cell 78:95-109.
10. Futai M, Tsuchiya T. 1987. Proton transport and proton-motive force in prokaryotic cells. In: Rosen BP, Silver S, editors. Ion Transport in Prokaryotes. London: Academic Press. p 3-83.
11. Harrop AJ, Hocknull MD, Lilly MD. 1989. Biotransformations in organic solvents: A difference between gram-positive and gram-negative bacteria. Biotechnol Lett 11:807-810.
12. Hewitt CJ, Lotte AB, McFarlane CM, Nienow AW. 1998. The use of flow cytometry to study the impact of fluid mechanical stress on Escherichia coli W3110 during continuous cultivation in an agitated bioreactor. Biotechnol Bioeng 63:705-711.
13. Hewitt CJ, Nebe-Von Caron G, Nienow AW, McFarlane CM. 1999a. Use of multistaining flow cytometry to characterise the physiological state of Escherichia coli W3110 in high cell density fed-batch cultures. Biotechnol Bioeng 63:705-711.
14. Hewitt CJ, Nebe-von-Caron G, Nienow AW, McFarlane CM. 1999b. The use of multiparameter flow cytometry to compare the physiological response of Escherichia coli W3110 to glucose limitation during batch, fed-batch and continuous culture cultivation. J Biotechnol 75:251-254.
15. Ingram LO. 1976. Adaptation of membrane lipids to alcohols. J Bacteriol 125:670-678.
16. Inoue A, Horikoshi K. 1989. A Pseudomonas thrives in high concentrations of toluene. Nature 338:264-266.
17. Isken S, de Bont JAM. 1996. Active efflux of toluene in a solvent-resistant bacterium. J Bacteriol 178:6056-6058.
18. Kieboom J, Dennis JJ, de Bont JAM, Zylstra GJ. 1998. Identification and molecular characterization of an efflux pump involved in Pseudomonas putida S12 solvent tolerance. J Biol Chem 273:85-91.
19. McDonald DL, Goldfine H. 1991. Effects of solvents and alcohols on the polar lipid composition of Clostridium butyricum under conditions of controlled lipid chain composition. Appl Environ Microbiol 57:3517-3521.
20. Nebe-Von Caron G, Badley RA. 1995. Viability assessment of bacteria in mixed populations using flow cytometry. J Microscopy 179:55-66.
21. Osborne SJ, Leaver J, Turner MK, Dunnill P. 1990. Correlation of biocatalytic activity in an organic-aqueous two liquid phase system with solvent concentration in the cell membrane. Enzy Micro Technol 12:281-291
22. Robertson BR, Button DK, Koch AL. 1998. Determination of the biomasses of small bacteria at low concentrations in a mixture of species with forward light scatter measurements by flow cytometry. Appl Environ Microbiol 64:3900-3909.
23. Sikkema J, Poolman B, Konings WN, De Bont JAM. 1992. Effects of the membrane action of tetralin on the functional and structural properties of artificial and bacterial membranes. J Bacteriol 174:2986-2992.
24. Shapiro HM. 1995. Practical Flow Cytometry, Third Edition. New York: Wiley-Liss.
25. Stafford DE, Yanagimachi KS, Stephanopoulos G. 2001. Metabolic engineering of indene bioconversion in Rhodococcus sp. Adv Biochem Eng Biotechnol 73:85-101.
26. Uribe SJ, Ramirez J, Pena A. 1985. Effects of β-pinene on yeast membrane functions. J Bacteriol 161:1196-1200.
27. Uribe SJ, Rangel P, Espfnola G, Aguirre G. 1990. Effects of cyclohexane, an industrial solvent, on the yeast Saccharomyces cerevisiae and on isolated yeast mitochondria. Appl Environ Microbiol 56:2114-2119.
28. Vermuë M, Sikkema J, Verheul A, Bakker R, Trampers J. 1993. Toxicity of homologous series of organic solvents for the Gram-positive bacteria Arthrobacter and Nocardia sp. and the gram-negative bacteria Acinetobacrer and Pseudomonas sp. Biotechnol Bioeng 42:747-758.
29. Walderhaug MO, Dosch DC, Epstein W. 1987. Potassium transport in bacteria. In: Rosen BP, Silver S, editors. Ion Transport in Prokaryotes. London: Academic Press. p 85-130.
30. Weber FJ, Isken S, de Bont JAM. 1994. Cis-trans isomerization of fatty acids as a defense mechanism of Pseudomonas putida strains to toxic concentrations of toluene. Microbiology 140:2013-2017.