Analysis of DNA Methylation in FFPE Tissues Using the MethyLight Technology

Methods in Molecular Biology

Volumn 724, Issue , 2011, Pages 191-204

  Dallol, Ashraf ^a   Al Ali, Waleed ^b   Al Shaibani, Amina ^b   Al Mulla, Fahd ^b  

^a KING ABDULAZIZ UNIVERSITY   (Saudi Arabia)

^b KUWAIT UNIVERSITY   (Kuwait)

Author keywords

CIMP; DNA; FFPE; Methylation; MethyLight; PCR; PMR

Indexed keywords

BISULFITE; DNA; FORMALDEHYDE; METHYLTRANSFERASE; PARAFFIN; PRIMER DNA; SOMATOMEDIN B; SULFITE;

ARTICLE; CHEMISTRY; CPG ISLAND; DNA METHYLATION; GENETICS; HUMAN; ISOLATION AND PURIFICATION; METABOLISM; METHODOLOGY; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; PROMOTER REGION; TISSUE FIXATION;

BASE SEQUENCE; CPG ISLANDS; DNA; DNA METHYLATION; DNA PRIMERS; FORMALDEHYDE; HUMANS; INSULIN-LIKE GROWTH FACTOR II; METHYLTRANSFERASES; MOLECULAR SEQUENCE DATA; PARAFFIN EMBEDDING; POLYMERASE CHAIN REACTION; PROMOTER REGIONS, GENETIC; SULFITES; TISSUE FIXATION;

EID: 79958258118     PISSN: 10643745     EISSN: 19406029     Source Type: Book Series    
DOI: 10.1007/978-1-61779-055-3_13     Document Type: Chapter

References (12)

1. Al-Mulla, F. (2007) Utilization of microarray platforms in clinical practice: an insight on the preparation and amplification of nucleic acids from frozen and fixed tissues. Methods Mol Biol. 382, 115–136.
2. Gagnon, J. F., Sanschagrin, F., Jacob, S., Tremblay, A. A., Provencher, L., Robert, J., et al. (2009) Quantitative DNA methylation analysis of laser capture microdissected formalin-fixed and paraffin-embedded tissues. Exp Mol Pathol. 8, 8.
3. Dietrich, D., Lesche, R., Tetzner, R., Krispin, M., Dietrich, J., Haedicke, W., et al. (2009) Analysis of DNA methylation of multiple genes in microdissected cells from formalin-fixed and paraffin-embedded tissues. J Histochem Cytochem. 57, 477–489.
4. Clark, S. J., Harrison, J., Paul, C. L., and Frommer, M. (1994) High sensitivity mapping of methylated cytosines. Nucleic Acids Res. 22, 2990–2997.
5. Eads, C. A. and Laird, P. W. (2002) Combined bisulfite restriction analysis (COBRA). Methods Mol Biol. 200, 71–85.
6. Herman, J. G., Graff, J. R., Myohanen, S., Nelkin, B. D., and Baylin, S. B. (1996) Methylation-specific PCR: a novel PCR assay for methylation status of CpG islands. Proc Natl Acad Sci USA. 93, 9821–9826.
7. Eads, C. A., Danenberg, K. D., Kawakami, K., Saltz, L. B., Blake, C., Shibata, D., et al. (2000) MethyLight: a high-throughput assay to measure DNA methylation. Nucleic Acids Res. 28, E32.
8. Issa, J. P. (2004) CpG island methylator phenotype in cancer. Nat Rev Cancer. 4, 988–993.
9. Widschwendter, M., Siegmund, K. D., Muller, H. M., Fiegl, H., Marth, C., Muller-Holzner, E., et al. (2004) Association of breast cancer DNA methylation profiles with hormone receptor status and response to tamoxifen. Cancer Res. 64, 3807–3813.
10. Weisenberger, D. J., Siegmund, K. D., Campan, M., Young, J., Long, T. I., Faasse, M. A., et al. (2006) CpG island methylator phenotype underlies sporadic microsatellite instability and is tightly associated with BRAF mutation in colorectal cancer. Nat Genet. 38, 787–793.
11. Ogino, S., Brahmandam, M., Kawasaki, T., Kirkner, G. J., Loda, M., and Fuchs, C. S. (2006) Epigenetic profiling of synchronous colorectal neoplasias by quantitative DNA methylation analysis. Mod Pathol. 19, 1083–1090.
12. Esteller, M. (2003) Relevance of DNA methylation in the management of cancer. Lancet Oncol. 4, 351–358.