Phos-tag-based analysis of myosin regulatory light chain phosphorylation in human uterine myocytes

PLoS ONE

Volumn 6, Issue 6, 2011, Pages

  Aguilar, Hector N ^a   Tracey, Curtis N ^a   Tsang, Siu Cheung F ^a   McGinnis, Justin M ^a   Mitchell, Bryan F ^a  

^a UNIVERSITY OF ALBERTA   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

CALPEPTIN; MYOSIN LIGHT CHAIN; MYOSIN LIGHT CHAIN KINASE; MYOSIN REGULATORY LIGHT CHAIN; OXYTOCIN; PHORBOL ESTER; RHO KINASE; UNCLASSIFIED DRUG; CELL EXTRACT; DIPEPTIDE; PHOSPHOPROTEIN; SERINE;

ARTICLE; CONTROLLED STUDY; ENZYME INDUCTION; ENZYME PHOSPHORYLATION; ENZYME STRUCTURE; FEMALE; HUMAN; HUMAN CELL; MUSCLE CELL; PHOSPHATE BINDING TAG; PROTEIN ANALYSIS; PROTEIN PHOSPHORYLATION; QUANTITATIVE ANALYSIS; UTERUS; BINDING SITE; CHEMISTRY; CYTOLOGY; DRUG EFFECT; ENZYMOLOGY; METABOLISM; PHOSPHORYLATION; PREGNANCY; REPRODUCIBILITY; SIGNAL TRANSDUCTION;

PHOS;

BINDING SITES; CELL EXTRACTS; DIPEPTIDES; FEMALE; HUMANS; MUSCLE CELLS; MYOSIN LIGHT CHAINS; MYOSIN-LIGHT-CHAIN KINASE; OXYTOCIN; PHORBOL ESTERS; PHOSPHOPROTEINS; PHOSPHORYLATION; PREGNANCY; REPRODUCIBILITY OF RESULTS; RHO-ASSOCIATED KINASES; SERINE; SIGNAL TRANSDUCTION; UTERUS;

EID: 79958250244     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0020903     Document Type: Article

References (45)

1. Hunter T, (2000) Signaling-2000 and beyond. Cell 100: 113-127.
2. Thingholm TE, Jensen ON, Larsen MR, (2009) Analytical strategies for phosphoproteomics. Proteomics 9: 1451-1468.
3. Kinoshita E, Kinoshita-Kikuta E, Takiyama K, Koike T, (2006) Phosphate-binding tag, a new tool to visualize phosphorylated proteins. Mol Cell Proteomics 5: 749-757.
4. Kinoshita E, Kinoshita-Kikuta E, Koike T, (2009) Separation and detection of large phosphoproteins using Phos-tag SDS-PAGE. Nat Protoc 4: 1513-1521.
5. Takeya K, Loutzenhiser K, Shiraishi M, Loutzenhiser R, Walsh MP, (2008) A highly sensitive technique to measure myosin regulatory light chain phosphorylation: the first quantification in renal arterioles. Am J Physiol Renal Physiol 294: F1487-1492.
6. Deswal S, Beck-Garcia K, Blumenthal B, Dopfer EP, Schamel WW, (2010) Detection of phosphorylated T and B cell antigen receptor species by Phos-tag SDS- and Blue Native-PAGE. Immunol Lett 130: 51-56.
7. Yang L, Xue Z, He Y, Sun S, Chen H, et al. (2010) A phos-tag-based approach reveals the extent of physiological endoplasmic reticulum stress. PLoS ONE 5: e11621.
8. Hosokawa T, Saito T, Asada A, Fukunaga K, Hisanaga S, (2010) Quantitative measurement of in vivo phosphorylation states of Cdk5 activator p35 by Phos-tag SDS-PAGE. Mol Cell Proteomics 9: 1133-1143.
9. Aguilar HN, Zielnik B, Tracey CN, Mitchell BF, (2010) Quantification of rapid Myosin regulatory light chain phosphorylation using high-throughput in-cell Western assays: comparison to Western immunoblots. PLoS ONE 5: e9965.
10. Sobieszek A, (1977) Ca-linked phosphorylation of a light chain of vertebrate smooth-muscle myosin. Eur J Biochem 73: 477-483.
11. Lebowitz EA, Cooke R, (1979) Phosphorylation of uterine smooth muscle myosin permits actin-activation. J Biochem 85: 1489-1494.
12. Kim HR, Appel S, Vetterkind S, Gangopadhyay SS, Morgan KG, (2008) Smooth muscle signalling pathways in health and disease. J Cell Mol Med 12: 2165-2180.
13. Takashima S, (2009) Phosphorylation of myosin regulatory light chain by myosin light chain kinase, and muscle contraction. Circ J 73: 208-213.
14. Haeberle JR, Hathaway DR, DePaoli-Roach AA, (1985) Dephosphorylation of myosin by the catalytic subunit of a type-2 phosphatase produces relaxation of chemically skinned uterine smooth muscle. J Biol Chem 260: 9965-9968.
15. Kamm KE, Stull JT, (2001) Dedicated myosin light chain kinases with diverse cellular functions. J Biol Chem 276: 4527-4530.
16. Ikebe M, Hartshorne DJ, (1985) Phosphorylation of smooth muscle myosin at two distinct sites by myosin light chain kinase. J Biol Chem 260: 10027-10031.
17. Ikebe M, Koretz J, Hartshorne DJ, (1988) Effects of phosphorylation of light chain residues threonine 18 and serine 19 on the properties and conformation of smooth muscle myosin. J Biol Chem 263: 6432-6437.
18. Tashiro Y, Matsumura S, Murakami N, Kumon A, (1984) The phosphorylation site for casein kinase II on 20,000-Da light chain of gizzard myosin. Arch Biochem Biophys 233: 540-546.
19. Colburn JC, Michnoff CH, Hsu LC, Slaughter CA, Kamm KE, et al. (1988) Sites phosphorylated in myosin light chain in contracting smooth muscle. J Biol Chem 263: 19166-19173.
20. Ikebe M, Hartshorne DJ, Elzinga M, (1987) Phosphorylation of the 20,000-dalton light chain of smooth muscle myosin by the calcium-activated, phospholipid-dependent protein kinase. Phosphorylation sites and effects of phosphorylation. J Biol Chem 262: 9569-9573.
21. Haeberle JR, Sutton TA, Trockman BA, (1988) Phosphorylation of two sites on smooth muscle myosin. Effects on contraction of glycerinated vascular smooth muscle. J Biol Chem 263: 4424-4429.
22. Sutton TA, Haeberle JR, (1990) Phosphorylation by protein kinase C of the 20,000-dalton light chain of myosin in intact and chemically skinned vascular smooth muscle. J Biol Chem 265: 2749-2754.
23. Barany K, Csabina S, de Lanerolle P, Barany M, (1987) Evidence for isoforms of the phosphorylatable myosin light chain in rat uterus. Biochim Biophys Acta 911: 369-371.
24. Csabina S, Barany M, Barany K, (1987) Comparison of myosin light chain phosphorylation in uterine and arterial smooth muscles. Comp Biochem Physiol B 87: 271-277.
25. Csabina S, Mougios V, Barany M, Barany K, (1986) Characterization of the phosphorylatable myosin light chain in rat uterus. Biochim Biophys Acta 871: 311-315.
26. Somlyo AP, Somlyo AV, (1998) From pharmacomechanical coupling to G-proteins and myosin phosphatase. Acta Physiol Scand 164: 437-448.
27. Somlyo AP, Somlyo AV, (1994) Signal transduction and regulation in smooth muscle. Nature 372: 231-236.
28. Bradley AB, Morgan KG, (1987) Alterations in cytoplasmic calcium sensitivity during porcine coronary artery contractions as detected by aequorin. J Physiol 385: 437-448.
29. Noda M, Yasuda-Fukazawa C, Moriishi K, Kato T, Okuda T, et al. (1995) Involvement of rho in GTP gamma S-induced enhancement of phosphorylation of 20 kDa myosin light chain in vascular smooth muscle cells: inhibition of phosphatase activity. FEBS Lett 367: 246-250.
30. Kitazawa T, Kobayashi S, Horiuti K, Somlyo AV, Somlyo AP, (1989) Receptor-coupled, permeabilized smooth muscle. Role of the phosphatidylinositol cascade, G-proteins, and modulation of the contractile response to Ca2+. J Biol Chem 264: 5339-5342.
31. Schoenwaelder SM, Burridge K, (1999) Evidence for a calpeptin-sensitive protein-tyrosine phosphatase upstream of the small GTPase Rho. A novel role for the calpain inhibitor calpeptin in the inhibition of protein-tyrosine phosphatases. J Biol Chem 274: 14359-14367.
32. Schoenwaelder SM, Petch LA, Williamson D, Shen R, Feng GS, et al. (2000) The protein tyrosine phosphatase Shp-2 regulates RhoA activity. Curr Biol 10: 1523-1526.
33. Tamura M, Nakao H, Yoshizaki H, Shiratsuchi M, Shigyo H, et al. (2005) Development of specific Rho-kinase inhibitors and their clinical application. Biochim Biophys Acta 1754: 245-252.
34. Ono-Saito N, Niki I, Hidaka H, (1999) H-series protein kinase inhibitors and potential clinical applications. Pharmacol Ther 82: 123-131.
35. Sasaki Y, Suzuki M, Hidaka H, (2002) The novel and specific Rho-kinase inhibitor (S)-(+)-2-methyl-1-[(4-methyl-5-isoquinoline)sulfonyl]-homopiperazine as a probing molecule for Rho-kinase-involved pathway. Pharmacol Ther 93: 225-232.
36. Erdodi F, Rokolya A, Barany M, Barany K, (1988) Phosphorylation of the 20,000-Da myosin light chain isoforms of arterial smooth muscle by myosin light chain kinase and protein kinase C. Arch Biochem Biophys 266: 583-591.
37. Wilson DP, Sutherland C, Borman MA, Deng JT, Macdonald JA, et al. (2005) Integrin-linked kinase is responsible for Ca2+-independent myosin diphosphorylation and contraction of vascular smooth muscle. Biochem J 392: 641-648.
38. Bengur AR, Robinson EA, Appella E, Sellers JR, (1987) Sequence of the sites phosphorylated by protein kinase C in the smooth muscle myosin light chain. J Biol Chem 262: 7613-7617.
39. Nishikawa M, Sellers JR, Adelstein RS, Hidaka H, (1984) Protein kinase C modulates in vitro phosphorylation of the smooth muscle heavy meromyosin by myosin light chain kinase. J Biol Chem 259: 8808-8814.
40. Nishikawa M, Hidaka H, Adelstein RS, (1983) Phosphorylation of smooth muscle heavy meromyosin by calcium-activated, phospholipid-dependent protein kinase. The effect on actin-activated MgATPase activity. J Biol Chem 258: 14069-14072.
41. Van Duuren BL, Tseng SS, Segal A, Smith AC, Melchionne S, et al. (1979) Effects of structural changes on the tumor-promoting activity of phorbol myristate acetate on mouse skin. Cancer Res 39: 2644-2646.
42. Kinoshita E, Kinoshita-Kikuta E, (2010) Improved Phos-tag SDS-PAGE under neutral pH conditions for advanced protein phosphorylation profiling. Proteomics.
43. Kinoshita E, Kinoshita-Kikuta E, Matsubara M, Yamada S, Nakamura H, et al. (2008) Separation of phosphoprotein isotypes having the same number of phosphate groups using phosphate-affinity SDS-PAGE. Proteomics 8: 2994-3003.
44. Turbedsky K, Pollard TD, Bresnick AR, (1997) A subset of protein kinase C phosphorylation sites on the myosin II regulatory light chain inhibits phosphorylation by myosin light chain kinase. Biochemistry 36: 2063-2067.
45. Umemoto S, Bengur AR, Sellers JR, (1989) Effect of multiple phosphorylations of smooth muscle and cytoplasmic myosins on movement in an in vitro motility assay. J Biol Chem 264: 1431-1436.