Benefits of heat treatment to the protease packed neutrophil for proteome analysis: Halting protein degradation

Proteomics

Volumn 11, Issue 12, 2011, Pages 2560-2564

  Kennedy, Susan A ^a   Scaife, Caitríona ^a   Dunn, Michael J ^a   Wood, Alfred E ^b   Watson, R William G ^a  

^a UNIVERSITY COLLEGE DUBLIN   (Ireland)

^b MATER MISERICORDIAE UNIVERSITY HOSPITAL   (Ireland)

Author keywords

2 DE; Heat treatment; Neutrophil; Proteolysis; Technology

Indexed keywords

BUFFER; PROTEINASE; PROTEOME; TRITON X 114;

AMINO ACID SEQUENCE; ARTICLE; CELL ISOLATION; HEAT TREATMENT; HUMAN; HUMAN CELL; IMMUNOBLOTTING; LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; MOLECULAR WEIGHT; NEUTROPHIL; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN DEGRADATION; PROTEOMICS; TWO DIMENSIONAL ELECTROPHORESIS;

CHROMATOGRAPHY, LIQUID; ELECTROPHORESIS, GEL, TWO-DIMENSIONAL; HOT TEMPERATURE; HUMANS; HYDROLASES; HYDROLYSIS; MASS SPECTROMETRY; NEUTROPHILS; PEPTIDE HYDROLASES; PROTEOME;

EID: 79958120578     PISSN: 16159853     EISSN: 16159861     Source Type: Journal    
DOI: 10.1002/pmic.201000625     Document Type: Article

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