A modified silver staining protocol for visualization of proteins compatible with matrix-assisted laser desorption/ionization and electrospray ionization-mass spectrometry

Electrophoresis

Volumn 21, Issue 17, 2000, Pages 3666-3672

  Yan, Jun X ^a   Wait, Robin ^b   Berkelman, Tom ^c   Harry, Rachel A ^a   Westbrook, Jules A ^a   Wheeler, Colin H ^a   Dunn, Michael J ^a  

^a HAREFIELD HOSPITAL   (United Kingdom)

^b UNIVERSITY OF OXFORD   (United Kingdom)

^c Amersham Biosciences   (United States)

Author keywords

Electrospray ionization time of flight; Mass spectrometry; Matrix assisted laser desorption ionization time of flight; Protein identification; Proteomics; Silver stain; Two dimensional gel electrophoresis

Indexed keywords

DESORPTION; ELECTROPHORESIS; ELECTROSPRAY IONIZATION; MASS SPECTROMETERS; MOLECULAR BIOLOGY; PROTEINS; SILVER; THREE DIMENSIONAL COMPUTER GRAPHICS; VISUALIZATION;

ELECTROSPRAY IONIZATION - TIME OF FLIGHT; MATRIX ASSISTED LASER DESORPTION IONIZATION; MATRIX-ASSISTED LASER DESORPTION/IONIZATION TIME-OF FLIGHT; MATRIXASSISTED LASER DESORPTION-IONIZATION (MALDI); PROTEIN IDENTIFICATION; PROTEOMICS; SILVER STAIN; SILVER STAINING; TIME-OF FLIGHT; TWO-DIMENSIONAL GEL ELECTROPHORESIS;

MASS SPECTROMETRY;

ANIMAL TISSUE; ARTICLE; IONIZATION; MASS SPECTROMETRY; NONHUMAN; PROTEIN ANALYSIS; QUANTITATIVE ASSAY; RAT; SILVER STAINING; TWO DIMENSIONAL ELECTROPHORESIS;

EID: 0033662168     PISSN: 01730835     EISSN: None     Source Type: Journal    
DOI: 10.1002/1522-2683(200011)21:17<3666::AID-ELPS3666>3.0.CO;2-6     Document Type: Article

References (25)