Purification and properties of family-10 endo-1,4-β-xylanase from Penicillium citrinum and structural organization of encoding gene

Journal of Bioscience and Bioengineering

Volumn 105, Issue 4, 2008, Pages 367-374

  Wakiyama, Motoki ^a   Tanaka, Hidenori ^a   Yoshihara, Koji ^a   Hayashi, Sachio ^a   Ohta, Kazuyoshi ^a  

^a UNIVERSITY OF MIYAZAKI   (Japan)

Author keywords

endo 1,4 xylanase; extracellular enzyme; glycoside hydrolase family 10; Penicillium citrinum; phylogenetic analysis; xylan

Indexed keywords

AMINO ACIDS; ENZYME ACTIVITY; GENE ENCODING; GENETIC ENGINEERING; PURIFICATION; YEAST;

EXTRACELLULAR ENZYMES; GLYCOSIDE HYDROLASE; PENICILLIUM CITRINUM; PHYLOGENETIC ANALYSIS; XYLANS;

CELL CULTURE;

COMPLEMENTARY DNA; ENDO 1,4 BETA XYLANASE; GENOMIC DNA; GLYCOSIDASE;

ARTICLE; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME PURIFICATION; GENE SEQUENCE; GENE STRUCTURE; GENETIC ORGANIZATION; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; OPEN READING FRAME; PENICILLIUM CITRINUM; PICHIA PASTORIS; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN EXPRESSION; SEQUENCE ALIGNMENT; SOUTHERN BLOTTING;

AMINO ACID SEQUENCE; BETA-GLUCOSIDASE; BETULA; ENDO-1,4-BETA XYLANASES; FUNGAL PROTEINS; GENES, FUNGAL; OPEN READING FRAMES; PENICILLIUM; PICHIA; RECOMBINANT PROTEINS; XYLANS;

FUNGI; PENICILLIUM CITRINUM; PICHIA PASTORIS;

EID: 43549087636     PISSN: 13891723     EISSN: None     Source Type: Journal    
DOI: 10.1263/jbb.105.367     Document Type: Article

References (25)

1. Puls J., and Schuseil J. Chemistry of hemicelluloses: relationship between hemicellulose structure and enzymes required for hydrolysis. In: Coughlan M.P., and Hazlewood G.P. (Eds). Hemicellulose and hemicellulase (1993), Portland Press, London 1-27
2. Coughlan M.P., and Hazlewood G.P. β-1,4-d-Xylan-degrading enzyme systems: biochemistry, molecular biology and applications. Biotechnol. Appl. Biochem. 17 (1993) 259-289
3. Wong K.K.Y., Tan L.U.L., and Saddler J.N. Multiplicity of β-1,4-xylanase in microorganisms: functions and applications. Microbiol. Rev. 52 (1988) 305-317
4. Henrissat B., and Bairoch A. Updating the sequence-based classification of glycosyl hydrolases. Biochem. J. 316 (1996) 695-696
5. Biely P., Vršanská M., Tenkanen M., and Kluepfel D. Endo-β-1,4-xylanase families: differences in catalytic properties. J. Biotechnol. 57 (1997) 151-166
6. Sato Y., Niimura Y., Yura K., and Gō M. Module-intron correlation and intron sliding in family F/10 xylanase genes. Gene 238 (1999) 93-101
7. Chávez R., Bull P., and Eyzaguirre J. The xylanolytic enzyme system from the genus Penicillium. J. Biotechnol. 123 (2006) 413-433
8. Chávez R., Almarza C., Schachter K., Peirano A., Bull P., and Eyzaguirre J. Structure analysis of the endoxylanase A gene from Penicillium purpurogenum. Biol. Res. 34 (2001) 217-226
9. Tanaka H., Nakamura T., Hayashi S., and Ohta K. Purification and properties of an extracellular endo-1,4-β-xylanase from Penicillium citrinum and characterization of the encoding gene. J. Biosci. Bioeng. 100 (2005) 623-630
10. Somogyi M. Notes on sugar determination. J. Biol. Chem. 195 (1952) 19-23
11. Lowry O.H., Rosebrough N.J., Farr A.L., and Randall R.J. Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193 (1951) 265-275
12. Laemmli U.K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227 (1970) 680-685
13. Ohta K., Akimoto H., Matsuda S., Toshimitsu D., and Nakamura T. Molecular cloning and sequence analysis of two endoinulinase genes from Aspergillus niger. Biosci. Biotechnol. Biochem. 62 (1998) 1731-1738
14. Sambrook J., and Russell D.W. Molecular cloning: a laboratory manual. 3rd ed. (2001), Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York
15. Akimoto H., Kiyota N., Kushima T., Nakamura T., and Ohta K. Molecular cloning and sequence analysis of an endoinulinase gene from Penicillium sp. strain TN-88. Biosci. Biotechnol. Biochem. 64 (2000) 2328-2335
16. Moriyama S., Tanaka H., Uwataki M., Muguruma M., and Ohta K. Molecular cloning and characterization of an exoinulinase gene from Aspergillus niger strain 12 and its expression in Pichia pastoris. J. Biosci. Bioeng. 96 (2003) 324-331
17. Serebryanyi V.A., Vavilova E.A., Chulkin A.M., and Vinetskii Y.P. Cloning of Penicillium canescens endo-1,4-β-xylanase gene and construction of multicopy strains. Appl. Biochem. Microbiol. 38 (2002) 420-426
18. Gurr S.J., Unkles S.E., and Kinghorn J.R. The structure and organization of nuclear genes of filamentous fungi. In: Kinghorn J.R. (Ed). Gene structure in eukaryotic microbes (1987), IRL Press, Oxford, United Kingdom 93-139
19. Haas H., Friedlin E., Stöffler G., and Redl B. Cloning and structural organization of a xylanase-encoding gene from Penicillium chrysogenum. Gene 126 (1993) 237-242
20. Schmidt A., Schlacher A., Steiner W., Schwab H., and Kratky C. Structure of the xylanase from Penicillium simplicissimum. Protein Sci. 7 (1998) 2081-2088
21. Kimura T., Suzuki H., Furuhashi H., Aburatani T., Morimoto K., Sakka K., and Ohmiya K. Molecular cloning, characterization, and expression analysis of the xynF3 gene from Aspergillus oryzae. Biosci. Biotechnol. Biochem. 66 (2002) 285-292
22. MacCabe A.P., Fernández-Espinar M.T., de Graaff L.H., Visser J., and Ramón D. Identification, isolation and sequence of the Aspergillus nidulans xlnC gene encoding the 34-kDa xylanase. Gene 175 (1996) 29-33
23. Bendtsen J.D., Nielsen H., von Heijne G., and Brunak S. Improved prediction of signal peptides: SignalP 3.0. J. Mol. Biol. 340 (2004) 783-795
24. Ito K., Ikemasu T., and Ishikawa T. Cloning and sequencing of the xynA gene encoding xylanase A of Aspergillus kawachii. Biosci. Biotechnol. Biochem. 56 (1992) 906-912
25. Chantasingh D., Pootanakit K., Champreda V., Kanokratana P., and Eurwilaichitr L. Cloning, expression, and characterization of a xylanase 10 from Aspergillus terreus (BCC129) in Pichia pastoris. Protein Expr. Purif. 46 (2006) 143-149