Acute bacterial meningitis cases diagnosed by culture and PCR in a children's hospital throughout a 9-year period (2000-2008) in Athens, Greece

Molecular Diagnosis and Therapy

Volumn 15, Issue 2, 2011, Pages 109-113

  Papavasileiou, Konstantina ^a   Papavasileiou, Eleni ^a   Tzanakaki, Georgina ^b   Voyatzi, Aliki ^a   Kremastinou, Jenny ^b   Chatzipanagiotou, Stylianos ^c  

^a PENTELI CHILDREN S HOSPITAL   (Greece)

^b NATIONAL SCHOOL OF PUBLIC HEALTH   (Greece)

^c UNIVERSITY OF ATHENS MEDICAL SCHOOL   (Greece)

Author keywords

Bacterial meningitis; children; Diagnostic tests; Diagnostics; Haemophilus influenzae; Haemophilus infections; Meningitis; Meningococcal infections; Neisseria meningitidis; PCR; Streptococcal infections; Streptococcus pneumoniae

Indexed keywords

C REACTIVE PROTEIN; GLUCOSE; PROTEIN;

ARTICLE; BACTERIAL MENINGITIS; BACTERIAL STRAIN; BACTERIAL VIRULENCE; BACTERIUM CULTURE; BACTERIUM ISOLATION; BLOOD SAMPLING; CEREBROSPINAL FLUID; CHILD HOSPITALIZATION; FEMALE; GLUCOSE BLOOD LEVEL; GREECE; HAEMOPHILUS INFLUENZAE; HUMAN; HUMAN CELL; INCIDENCE; LEUKOCYTE COUNT; MAJOR CLINICAL STUDY; MALE; NEISSERIA MENINGITIDIS; NONHUMAN; PEDIATRIC HOSPITAL; POLYMERASE CHAIN REACTION; PREDICTIVE VALUE; PRIORITY JOURNAL; PROTEIN BLOOD LEVEL; SENSITIVITY AND SPECIFICITY; STREPTOCOCCUS PNEUMONIAE;

BACTERIA (MICROORGANISMS); HAEMOPHILUS; HAEMOPHILUS INFLUENZAE; NEISSERIA MENINGITIDIS; NEISSERIA MENINGITIDIS SEROGROUP B; STREPTOCOCCUS; STREPTOCOCCUS GROUP B; STREPTOCOCCUS PNEUMONIAE;

EID: 79957860661     PISSN: 11771062     EISSN: 11792000     Source Type: Journal    
DOI: 10.1007/BF03256400     Document Type: Article

References (16)

1. World Health Organization. The world health report 2000 - health systems: improving performance [online]. Available from URL: http://www.who.int/whr/ 2000/en/index.html [Accessed 2011 Mar 8]
2. Jódar L, Feavers IM, Salisbury D, et al. Development of vaccines against meningococcal disease. Lancet 2002; 359 (9316): 1499-508 (Pubitemid 34457772)
3. Finn A. Bacterial polysaccharide-protein conjugate vaccines. Br Med Bull 2004; 70: 1-14 (Pubitemid 40069369)
4. Harrison LH. Vaccine prevention of meningococcal disease: making slow progress. Clin Infect Dis 2006; 43 (11): 1395-7 (Pubitemid 44845638)
5. Feigin RD, Pearlman E. Bacterial meningitis beyond the neonatal period. In: Feigin RD, Demler GJ, Cherry JD, et al., editors. Textbook of pediatric infectious diseases. 5th ed. Philadelphia (PA): Saunders, 2004: 443-74
6. Sáez-Llorens X, McCracken Jr GH. Bacterial meningitis in children. Lancet 2003; 361 (9375): 2139-48 (Pubitemid 36782263)
7. Peltola H. Worldwide Haemophilus influenzae type b disease at the beginning of the 21st century: global analysis of the disease burden 25 years after the use of the polysaccharide vaccine and a decade after the advent of conjugates. Clin Microbiol Rev 2000: 302-17
8. Centers for Disease Control and Prevention. Progress toward elimination of Haemophilus influenza type b invasive disease among infants and children: United States, 1998-2000. Morb Mortal Wkly Rep 2002; 51: 234-9
9. Clinical and Laboratory Standards Institute [CLSI]. Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically; approved standard - eighth edition [CLSI document M07-A8]. Wayne (PA): CLSI, 2009 Jan
10. Tzanakaki G, Tsopanomichalou M, Kesanopoulos K, et al. Simultaneous single-tube PCR assay for the detection of N. meningitidis, H. influenzae type b and streptococcus pneumoniae. Clin Microb Infect 2005; 11: 386-90
11. Drakopoulou Z, Kesanopoulos K, Sioumala M, et al. Simultaneous single-tube PCR-based assay for the direct identification of the five most common meningococcal serogroups from clinical samples. FEMS Immunol Med Microbiol 2008; 53 (2): 178-82 (Pubitemid 351832183)
12. Sioumala M, Tzanakaki G, Levidiotou S, et al. Development of two stepdown multiplex PCR for the simultaneous detection on nine main serotypes of Streptococcus pneumoniae directly in clinical samples. Acta Microbiol Hellenica 2007; 52 (3): 173-9 (Pubitemid 47605750)
13. National Meningitis Reference Laboratory, Greek National School of Public Health. National Meningitis Reference Laboratory annual report for the year 2009 [in Greek; online]. Available from URL: http://www.nsph.gr/files/ Apologismos%20EKAM%202009.pdf [Accessed 2010 Oct]
14. Schuurman T, de Boer RF, Kooistra-Smid AM, et al. Prospective study of use of PCR amplification and sequencing of 16S ribosomal DNA from cerebrospinal fluid for diagnosis of bacterial meningitis in a clinical setting. J Clin Microbiol 2004; 42 (2): 734-40 (Pubitemid 38222655)
15. Matos Jde A, Madureira DJ, Rebelo MC, et al. Diagnosis of Streptococcus pneumoniae meningitis by polymerase chain reaction amplification of the gene for pneumolysin. Mem Inst Oswaldo Cruz 2006; 101 (5): 559-63 (Pubitemid 44285801)
16. Rebelo MC, Boente RF, Matos Jde A, et al. Assessment of a two-step nucleic acid amplification assay for detection of Neisseria meningitides followed by capsular genogrouping. Mem Inst Oswaldo Cruz 2006; 101 (7): 809-13 (Pubitemid 44800163)