Simultaneous detection of nine serotypes of Streptococcus pneumoniae using stepdown multiplex PCR

Acta Microbiologica Hellenica

Volumn 52, Issue 3, 2007, Pages 173-179

  Sioumala, M ^a   Tzanakaki, G ^a   Kesanopoulos, K ^a   Levidiotou Stefanou, S ^b   Kourea Kremastinou, J ^a  

^a NSPH   (Greece)

^b UNIVERSITY OF IOANNINA   (Greece)

Author keywords

Multiplex PCR; Sirotypes; Streptococcus pneumoniae

Indexed keywords

ARTICLE; BLOOD CULTURE; BLOOD SAMPLING; BODY FLUID; BRONCHIAL FLUID; METHODOLOGY; MULTIPLEX POLYMERASE CHAIN REACTION; NONHUMAN; PLEURA FLUID; SEROTYPE; STREPTOCOCCUS PNEUMONIAE;

STREPTOCOCCUS PNEUMONIAE;

EID: 35348942307     PISSN: 04389573     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (16)

1. CDC. Prevention of pneumococcal disease recommendations of the Advisory Committee on Immunization Practices (ACIP). 1997, 46
2. Hausdorff WP, Feikin DR, Klugman KP. Epidemiological differences among pneumococcal serotypes. Lancet Infect Dis 2005, 5:83-93
3. Hausdorff WP, Bryant J, Kloek C, Paradiso PR, Siber GR. The contribution of specific pneumococcal serogroups to different disease manifestations: Implications for conjugate vaccine formulation and use, part II. Clin Infect Dis 2000, 30:122-140
4. Hausdorff WP, Siber G, Paradiso PR. Geographical differences in invasive pneumococcal disease rates and serotype frequency in young children. Lancet 2001, 357:950-952
5. Swartz MN. Bacterial meningitis - a view of the past 90 years. N Engl J Med 2004, 351:1826-1828
6. Henrichsen J. Six newly recognized types of Streptococcus pneumoniae. J Clin Microbiol 1995, 33:2759-2762
7. Finn A, Jenkinson HF. The pneumococcus: Old man's friend' and children's foe. Microbiology 2006, 152:281-283
8. Poehling KA, Talbot TR, Griffin MR, et al. Invasive pneumococcal disease among infants before and after introduction of pneumococcal conjugate vaccine. Jama 2006, 295:1668-1674
9. Lexau CA, Lynfield R, Danila R, et al. Changing epidemiology of invasive pneumococcal disease among older adults in the era of pediatric pneumococcal conjugate vaccine. JAMA 2005, 294:2043-2051
10. Tzanakaki G, Tsopanomichalou M, Kesanopoulos K, Matzourani R, Sioumala M, Tabaki A, Kremastinou J. Simultaneous single-tube PCR assay for the detection of Neisseria meningitidis, Haemophilus influenzae type b and Streptococcus pneumoniae. Clin Microbiol Infect 2005, 11:386-390
11. Zambarti G, Druetta A, Poure C. Rapid diagnosis of Mycobacterium tuberculosis infections by an ELISA-like detection of PCR products. Mol Cell Probes 1985, 9:91-99
12. Zuker M. Mfold web server for nucleic acid folding and hybridization prediction. Nucleic Acids Res 2003, 31:3406-3415
13. Brito DA, Ramirez M, de Lencastre H. Serotyping Streptococcus pneumoniae by multiplex PCR. J Clin Microbiol 2003, 41:2378-2384
14. Lawrence ER, Griffiths DB, Martin SA, George RC, Hall LM. Evaluation of semiautomated multiplex PCR assay for determination of Streptococcus pneumoniae serotypes and serogroups. J Clin Microbiol 2003, 41: 601-607
15. Shrestha NK, Tuohy MJ, Hall GS, Isada CM, Procop GW. Rapid identification of Staphylococcus aureus and the mecA gene from BacT/ALERT blood culture bottles by using the LightCycler system. J Clin Microbiol 2002, 40:2659-2661
16. O'Halloran DM, Cafferkey MT. Multiplex PCR for identification of seven Streptococcus pneumoniae serotypes targeted by a 7-valent conjugate vaccine. J Clin Microbiol 2005, 43(7):3487-3490