Isolation of high-quality total rna from rumen anaerobic bacteria and fungi, and subsequent detection of glycoside hydrolases

Canadian Journal of Microbiology

Volumn 57, Issue 7, 2011, Pages 590-598

  Wang, Pan ^a,b   Qi, Meng ^a   Barboza, Perry ^c   Leigh, Mary Beth ^c   Ungerfeld, Emilio ^a   Selinger, L Brent ^b   Mcallister, Tim A ^a   Forster, Robert J ^a  

^a LETHBRIDGE RESEARCH CENTRE   (Canada)

^b UNIVERSITY OF LETHBRIDGE   (Canada)

^c UNIVERSITY OF ALASKA FAIRBANKS   (United States)

Author keywords

Gene expression; Isolation; Metatranscriptomics; RNA quality; Rumen; Total RNA

Indexed keywords

ISOLATION; METATRANSCRIPTOMICS; RNA QUALITY; RUMEN; TOTAL RNA;

BACTERIA; DYES; ECOSYSTEMS; FUNGI; GENES; LIQUID NITROGEN; PHENOLS; RNA; SUGARS; TRANSCRIPTION;

POLYMERASE CHAIN REACTION;

BACTERIAL RNA; FUNGAL RNA; GLYCOSIDASE; MESSENGER RNA;

ANOXIC CONDITIONS; ARRAY; BACTERIUM; CHLOROFORM; EUKARYOTE; FERMENTATION; FUNGUS; GENE EXPRESSION; GENETIC ANALYSIS; HYDROLYSIS; LIMITING FACTOR; NITROGEN; PHENOL; POLYMERASE CHAIN REACTION; STABILIZATION;

ANAEROBIC BACTERIUM; ANIMAL TISSUE; ARTICLE; FUNGUS; NONHUMAN; PRIORITY JOURNAL; RNA ISOLATION; RUMINANT STOMACH;

ANIMALS; BACTERIA, ANAEROBIC; ECOSYSTEM; FUNGI; GLYCOSIDE HYDROLASES; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, BACTERIAL; RNA, FUNGAL; RUMEN;

EUKARYOTA; FUNGI;

EID: 79957856616     PISSN: 00084166     EISSN: 14803275     Source Type: Journal    
DOI: 10.1139/w11-048     Document Type: Article

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