Cloning and overexpression of aprE3-17 encoding the major fibrinolytic protease of Bacillus licheniformis CH 3-17

Biotechnology and Bioprocess Engineering

Volumn 16, Issue 2, 2011, Pages 352-359

  Jo, Hyeon Deok ^a   Kwon, Gun Hee ^a   Park, Jae Yong ^b   Cha, Jaeho ^c   Song, Young Sun ^d   Kim, Jeong Hwan ^a  

^a Gyeongsang National University   (South Korea)

^b Catholic University of Daegu   (South Korea)

^c Pusan National University   (South Korea)

^d Inje University   (South Korea)

Author keywords

bacilli; Bacillus licheniformis; fermented soyfood; fibrinolytic enzyme; gene expression

Indexed keywords

BACILLUS LICHENIFORMIS; BACILLUS SUBTILIS; CULTURE MEDIUM; CULTURE SUPERNATANT; DNA SEQUENCING; ESCHERICHIA COLI (E. COLI); FERMENTED SOYFOOD; FIBRINOLYTIC ACTIVITY; FIBRINOLYTIC ENZYMES; OPTIMUM PH; OVER-EXPRESSION; PURIFIED ENZYME; SERINE PROTEASE; SHUTTLE VECTOR; SUBTILIS;

AMINO ACIDS; BACTERIOLOGY; CLONING; ENCODING (SYMBOLS); ENZYMES; ESCHERICHIA COLI; GENE ENCODING; GENE EXPRESSION; POLYMERASE CHAIN REACTION; PURIFICATION;

BACILLI;

FIBROLASE;

APRE3 17 GENE; ARTICLE; BACILLUS LICHENIFORMIS; BACTERIAL GENE; BACTERIAL STRAIN; CONTROLLED STUDY; DNA SEQUENCE; ENZYME ACTIVITY; ENZYME DEGRADATION; ENZYME PURIFICATION; EXPRESSION VECTOR; GENE OVEREXPRESSION; HYDROLYSIS; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; PH; POLYMERASE CHAIN REACTION; SEQUENCE HOMOLOGY;

BACILLI (CLASS); BACILLUS (BACTERIUM); BACILLUS LICHENIFORMIS; BACILLUS SUBTILIS; ESCHERICHIA COLI;

EID: 79957807700     PISSN: 12268372     EISSN: 19763816     Source Type: Journal    
DOI: 10.1007/s12257-010-0328-0     Document Type: Article

References (16)

1. N. -S. Choi S. -K. Ju T. Y. Lee K. -S. Yoon K. -T. Chang P. J. Maeng S. -H. Kim 2005 Miniscale identification and characterization of subtilisins from Bacillus sp. strains J. Microbiol. Biotechnol. 15 537 543 1:CAS:528: DC%2BD2MXnvF2gurc%3D (Pubitemid 41015017)
2. Y. Peng Q. Huang R. H. Zhang Y. Z. Zhang 2003 Purification and characterization of a fibrinolytic enzyme produced by Bacillus amyloliquefaciens DC-4 screened from douchi, a traditional Chinese soybean food Comp. Biochem. Physiol. part B 134 45 52 10.1016/S1096-4959(02)00183-5 (Pubitemid 36078265)
3. H. Sumi H. Hamada H. Tsushima H. Mihara H. Muraki 1987 A novel fibrinolytic enzyme (NK) in the vegetable cheese Natto; a typical and popular soybean food in the Japanese diet Experientia 43 1110 1111 10.1007/BF01956052 1:CAS:528:DyaL1cXitVemsrw%3D
4. Y. Peng X. Yang Y. Zhang 2005 Microbial fibrinolytic enzymes: An overview of source, production, properties, and thrombolytic activity in vivo Appl. Microbial. Biotechnol. 69 126 132 10.1007/s00253-005-0159-7 1:CAS:528: DC%2BD2MXhtlWktrfF (Pubitemid 41795085)
5. C. T. Wang B. P. Ji B. L. R. Nout P. L. Li H. Ji L. F. Chen 2006 Purification and characterization of a fibrinolytic enzyme of Bacillus subtilis DC33, isolated from Chinese traditional douch J. Ind. Microbial. Biotechnol. 33 750 758 10.1007/s10295-006-0111-6 1:CAS:528:DC%2BD28Xnt1aisLg%3D (Pubitemid 44106681)
6. G. -H. Kwon H. -A. Lee J. -Y. Park J. S. Kim J. Lim C. -S. Park D. Y. Kwon J. H. Kim 2009 Development of a RAPD-PCR method for identification of Bacillus species isolated from Chunggukjang Int. J. Food Microbiol. 129 282 287 10.1016/j.ijfoodmicro.2008.12.013 1:CAS:528:DC%2BD1MXhs1amtb8%3D
7. G. M. Kim A. R. Lee K. W. Lee J. -Y. Park J. Chun J. Cha Y. -S. Song J. H. Kim 2009 Characterization of a 27 kDa fibrinolytic enzyme from Bacillus amyloliquefaciens CH51 isolated from cheonggukjang J. Microbiol. Biotechnol. 19 997 1004 10.4014/jmb.0811.600 1:CAS:528:DC%2BD1MXhtlansrnM
8. N. -S. Choi K. -T. Chang P. J. Maeng S. -H. Kim 2004 Cloning, expression, and fibrin (ogen)olytic properties of a subtilisin DJ-4 gene from Bacillus sp. DJ-4 FEMS. Microbiol. Lett. 236 325 331 10.1111/j.1574-6968.2004.tb09665.x 1:CAS:528:DC%2BD2cXls1ajtb8%3D
9. S. J. Jeong G. H. Kwon J. Chun J. S. Kim C. S. Park D. Y. Kwon J. H. Kim 2007 Cloning of fibrinolytic enzyme gene from Bacillus subtilis isolated from Cheonggukjang and its expression in protease-deficient Bacillus subtilis strains J. Microbiol. Biotechnol. 17 1018 1023 1:CAS:528:DC%2BD2sXosFSnsrw%3D (Pubitemid 47162067)
10. X. C. Wu W. Lee L. Tran S. L. Wong 1991 Engineering a Bacillus subtilis expression-secretion system with a strain deficient in six extracellular proteases J. Bacteriol. 173 4952 4958 1:CAS:528:DyaK3MXmtVShsbY%3D
11. M. M. Bradford 1976 Rapid and sensitive methods for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding Anal. Biochem. 72 248 254 10.1016/0003-2697(76)90527-3 1:CAS:528: DyaE28XksVehtrY%3D
12. U. K. Laemmli 1970 Cleavage of structural proteins during the assembly of the head of bacteriophage T4 Nature (London) 227 680 685 10.1038/227680a0 1:CAS:528:DC%2BD3MXlsFags7s%3D
13. C. Wang M. Du D. Zheng F. Kong G. Zu Y. Feng 2009 Purification and characterization of nattokinase from Bacillus subtilis Natto B-12 J. Agric. Food Chem. 57 9722 9729 10.1021/jf901861v 1:CAS:528:DC%2BD1MXhtFyqsrvE
14. R. Agrebi A. Haddar M. Hajji F. Frikha L. Manni K. Jellouli M. Nasri 2009 Fibrinolytic enzymes from a newly isolated marine bacterium Bacillus subtilis A26: Characterization and statistical media optimization Can. J. Microbiol. 55 1049 1061 10.1139/W09-057 1:CAS:528:DC%2BD1MXht1ant7nK
15. N. -S. Choi D. -M. Chung C. H. Ryu K. -S. Yoon P. J. Maeng S. -H. Kim 2006 Identification of three extracellular proteases from Bacillus subtilis KCTC 3014 J. Microbiol. Biotechnol. 16 457 464 1:CAS:528:DC%2BD28XjvFemu78%3D
16. S. -B. Kim D. -W. Lee C. -I. Cheigh E. -A. Choe S. -J. Lee Y. -H. Hong H. -J. Choi Y. -R. Pyun 2006 Purification and characterization of a fibrinolytic subtilisin-like protease of Bacillus subtilis TP-6 from an Indonesian fermented soybean, Tempeh J. Ind. Microbiol. Biotechnol. 33 436 444 10.1007/s10295-006- 0085-4 1:CAS:528:DC%2BD28XjvFSmsr8%3D