Interaction between the RNA binding domains of Ser-Arg splicing factor 1 and U1-70K snRNP protein determines early spliceosome assembly

Proceedings of the National Academy of Sciences of the United States of America

Volumn 108, Issue 20, 2011, Pages 8233-8238

  Cho, Suhyung ^a   Hoang, Amy ^a   Sinha, Rahul ^b   Zhong, Xiang Yang ^a   Fu, Xiang Dong ^a   Krainer, Adrian R ^b   Ghosh, Gourisankar ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b Howard Hughes Medical Institute Cold Spring Harbor Laboratory Cold Spring Harbor   (United States)

Author keywords

Exonic splicing enhancer; Protein phosphorylation; RNA splicing; Spliceosome complex

Indexed keywords

GLUTATHIONE TRANSFERASE; MESSENGER RNA PRECURSOR; RNA; SERINE ARGININE RICH PROTEIN; SERINE ARGININE RICH SPLICING FACTOR 1; SMALL NUCLEAR RIBONUCLEOPROTEIN; TRANSFER RNA; UNCLASSIFIED DRUG;

ARTICLE; BINDING SITE; DEPHOSPHORYLATION; EXON; EXONIC SPLICING ENHANCER; MAMMAL CELL; MOLECULAR INTERACTION; PRIORITY JOURNAL; PROTEIN DOMAIN; PROTEIN PHOSPHORYLATION; PROTEIN PURIFICATION; REGULATORY SEQUENCE; RNA BINDING; RNA SPLICING; SPLICEOSOME;

BINDING SITES; HELA CELLS; HUMANS; NUCLEAR PROTEINS; PHOSPHORYLATION; PROTEIN BINDING; RIBONUCLEOPROTEIN, U1 SMALL NUCLEAR; RNA PRECURSORS; RNA SPLICING; RNA-BINDING PROTEINS; SPLICEOSOMES;

MAMMALIA;

EID: 79957784833     PISSN: 00278424     EISSN: 10916490     Source Type: Journal    
DOI: 10.1073/pnas.1017700108     Document Type: Article

References (32)

1. Black DL (2003) Mechanisms of alternative pre-messenger RNA splicing. Annu Rev Biochem 72:291-336.
2. Liu HX, Zhang M, Krainer AR (1998) Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins. Genes Dev 12:1998-2012. (Pubitemid 28323874)
3. Blencowe BJ (2000) Exonic splicing enhancers: mechanism of action, diversity and role in human genetic diseases. Trends Biochem Sci 25:106-110.
4. Staknis D, Reed R (1994) SR proteins promote the first specific recognition of Pre-mRNA and are present together with the U1 small nuclear ribonucleoprotein particle in a general splicing enhancer complex. Mol Cell Biol 14:7670-7682. (Pubitemid 24326433)
5. Cao W, Jamison SF, Garcia-Blanco MA (1997) Both phosphorylation and dephosphorylation of ASF/SF2 are required for pre-mRNA splicing in vitro. RNA 3:1456-1467. (Pubitemid 28006720)
6. Boukis LA, Liu N, Furuyama S, Bruzik JP (2004) Ser/Arg-rich protein-mediated communication between U1 and U2 small nuclear ribonucleoprotein particles. J Biol Chem 279:29647-29653. (Pubitemid 38915848)
7. Xiao SH, Manley JL (1997) Phosphorylation of the ASF/SF2 RS domain affects both protein-protein and protein-RNA interactions and is necessary for splicing. Gene Dev 11:334-344.
8. Chen CD, Kobayashi R, Helfman DM (1999) Binding of hnRNP H to an exonic splicing silencer is involved in the regulation of alternative splicing of the rat beta-tropomyosin gene. Gene Dev 13:593-606. (Pubitemid 29131883)
9. Rooke N, Markovtsov V, Cagavi E, Black DL (2003) Roles for SR proteins and hnRNP A1 in the regulation of c-src exon N1. J Mol Cell Biol 23:1874-1884. (Pubitemid 36278802)
10. Long JC, Caceres JF (2009) The SR protein family of splicing factors: master regulators of gene expression. Biochem J 417:15-27.
11. Zhong XY, Wang P, Han J, Rosenfeld MG, Fu XD (2009) SR proteins in vertical integration of gene expression from transcription to RNA processing to translation. Mol Cell 35:1-10.
12. Aubol BE, et al. (2003) Processive phosphorylation of alternative splicing factor/splicing factor 2. Proc Natl Acad Sci USA 100:12601-12606. (Pubitemid 37339945)
13. Velazquez-Dones A, et al. (2005) Mass spectrometric and kinetic analysis of ASF/SF2 phosphorylation by SRPK1 and Clk/Sty. J Biol Chem 280:41761-41768. (Pubitemid 41832239)
14. Ngo JC, et al. (2005) Interplay between SRPK and Clk/Sty kinases in phosphorylation of the splicing factor ASF/SF2 is regulated by a docking motif in ASF/SF2. Mol Cell 20 (1):77-89. (Pubitemid 41396564)
15. Misteli T, et al. (1998) Serine phosphorylation of SR proteins is required for their recruitment to sites of transcription in vivo. J Cell Biol 143:297-307. (Pubitemid 28487851)
16. Kohtz JD, et al. (1994) Protein-protein interactions and 5′-splice-site recognition in mammalian mRNA precursors. Nature 368:119-124.
17. Cao W, Garcia-Blanco MA (1998) A serine/arginine-rich domain in the human U1 70k protein is necessary and sufficient for ASF/SF2 binding. J Biol Chem 273 (32):20629-20635. (Pubitemid 28377634)
18. Yeakley JM, et al. (1999) Phosphorylation regulates in vivo interaction and molecular targeting of serine/arginine-rich pre-mRNA splicing factors. J Cell Biol 145:447-455. (Pubitemid 29215710)
19. Shen H, Kan JL, Green MR (2004) Arginine-serine-rich domains bound at splicing enhancers contact the branchpoint to promote prespliceosome assembly. Mol Cell 13:367-376. (Pubitemid 38229809)
20. Shen H, Green MR (2004) A pathway of sequential arginine-serine-rich domain-splicing signal interactions during mammalian spliceosome assembly. Mol Cell 16:363-373. (Pubitemid 39504791)
21. Ghigna C, et al. (2005) Cell motility is controlled by SF2/ASF through alternative splicing of the Ron protooncogene. Mol Cell 20:881-890. (Pubitemid 41814877)
22. Cartegni L, Krainer AR (2002) Disruption of an SF2/ASF-dependent exonic splicing enhancer in SMN2 causes spinal muscular atrophy in the absence of SMN1. Nat Genet 30:377-384.
23. Liu HX, Cartegni L, Zhang MQ, Krainer AR (2001) A mechanism for exon skipping caused by nonsense or missense mutations in BRCA1 and other genes. Nat Genet 27:55-58. (Pubitemid 32044518)
24. Cazalla D, et al. (2002) Nuclear export and retention signals in the RS domain of SR proteins. Mol Cell Biol 22:6871-6882.
25. Caceres JF, Krainer AR (1993) Functional analysis of pre-mRNA splicing factor SF2/ASF structural domains. EMBO J 12:4715-4726. (Pubitemid 23330277)
26. Shi Y, Reddy B, Manley JL (2006) PP1/PP2A phosphatases are required for the second step of Pre-mRNA splicing and target specific snRNP proteins. Mol Cell 23:819-829. (Pubitemid 44344517)
27. Xiao SH, Manley JL (1998) Phosphorylation-dephosphorylation differentially affects activities of splicing factor ASF/SF2. EMBO J 17:6359-6367.
28. Shin C, Feng Y, Manley JL (2004) Dephosphorylated SRp38 acts as a splicing repressor in response to heat shock. Nature 427:553-558. (Pubitemid 38209114)
29. Lin S, Xiao R, Sun P, Xu X, Fu XD (2005) Dephosphorylation-dependent sorting of SR splicing factors during mRNP maturation. Mol Cell 20:413-425. (Pubitemid 41572298)
30. Wahl MC, Will CL, Luhrmann R (2009) The spliceosome: Design principles of a dynamic RNP machine. Cell 136:701-718.
31. Zhu J, Krainer AR (2000) Pre-mRNA splicing in the absence of an SR protein RS domain. Gene Dev 14:3166-3178. (Pubitemid 32015134)
32. Das R, Reed R (1999) Resolution of the mammalian E complex and the ATP-dependent spliceosomal complexes on native agarose mini-gels. RNA 5:1504-1508. (Pubitemid 29530495)