Role of ComFA in controlling the DNA uptake rate during transformation of competent Bacillus subtilis

Journal of Bioscience and Bioengineering

Volumn 111, Issue 6, 2011, Pages 618-623

  Takeno, Masaomi ^a   Taguchi, Hisataka ^a   Akamatsu, Takashi ^a  

^a SOJO UNIVERSITY   (Japan)

Author keywords

Bacillus subtilis; Brownian ratchet; ComFA; Competent transformation; DNA uptake

Indexed keywords

ATP-ASE ACTIVITY; BACILLUS SUBTILIS; BROWNIAN RATCHET; COMFA; COMPETENT TRANSFORMATION; COTRANSFORMATION; DELETION MUTANTS; LYSATES; RELATIVE EFFICIENCY; TRANSFORMANTS; UPTAKE RATE; WILD TYPES;

BACILLI; BACTERIOLOGY; POLYMERASE CHAIN REACTION;

DNA;

ADENOSINE TRIPHOSPHATASE; BACTERIAL DNA;

ARTICLE; BACILLUS SUBTILIS; BACTERIUM MUTANT; BACTERIUM TRANSFORMATION; COMPARATIVE MOLECULAR FIELD ANALYSIS; CONTROLLED STUDY; NONHUMAN; POLYMERASE CHAIN REACTION;

BACILLUS SUBTILIS; DNA, BACTERIAL; GENOTYPE; PLASMIDS; POINT MUTATION; PROTOPLASTS; SEQUENCE ANALYSIS, DNA; SEQUENCE DELETION; TRANSFORMATION, BACTERIAL;

BACILLUS SUBTILIS;

EID: 79957614139     PISSN: 13891723     EISSN: 13474421     Source Type: Journal    
DOI: 10.1016/j.jbiosc.2011.02.006     Document Type: Article

References (39)

1. Akamatsu T., Taguchi H. Incorporation of the whole chromosomal DNA in protoplast lysates into competent cells of Bacillus subtilis. Biosci. Biotechnol. Biochem. 2001, 65:823-829.
2. Priest F.G. Systematics and ecology of Bacillus subtilis, p. 3-16. Bacillus subtilis and other gram-positive bacteria 1993, American Society for Microbiology, Washington, D.C. A.L. Sonnenshein, J.A. Hoch, R. Losick (Eds.).
3. Wilson G.A., Young F.E. Intergenotic transformation of the Bacillus subtilis genospecies. J. Bacteriol. 1972, 111:705-716.
4. Dubnau D. Binding and transport of transforming DNA by Bacillus subtilis: the role of type-IV pilin-like proteins-a review. Gene 1997, 192:191-198.
5. Hahn J., Albano M., Dubnau D. Isolation and characterization of competence mutants in Bacillus subtilis. J. Bacteriol. 1987, 169:3104-3109.
6. Hahn J., Inamine G., Kozlov Y., Dubnau D. Characterization of comE, a late competence operon of Bacillus subtilis required for the binding and uptake of transforming DNA. Mol. Microbiol. 1993, 10:99-111.
7. Hahn J., Kong L., Dubnau D. The regulation of competence transcription factor synthesis constitutes a critical control point in the regulation of competence in Bacillus subtilis. J. Bacteriol. 1994, 176:5753-5761.
8. Van Sinderen D., ten Berg A., Hayema B.J., Hamoen L., Venema G. Molecular cloning and sequence of comK, a gene required for genetic competence in Bacillus subtilis. Mol. Microbiol. 1994, 11:695-703.
9. Van Sinderen D., Venema G. comK acts as an autoregulatory control switch in the signal transduction route to competence in Bacillus subtilis. J. Bacteriol. 1994, 176:5762-5770.
10. Van Sinderen D., Luttinger A., Kong L., Dubnau D., Venema G., Hamoen L. comK encodes the competence transcription factor (CTF), the key regulatory protein for competence development in Bacillus subtilis. Mol. Microbiol. 1995, 15:455-462.
11. Burton B., Dubnau D. Membrane-associated DNA transport machines. Cold Spring Harb. Perspect. Biol. 2010, 2:a000406.
12. Londoño-Vallejo J.A., Dubnau D. comF, a Bacillus subtilis late competence locus, encodes a protein similar to ATP-dependent RNA/DNA helicases. Mol. Microbiol. 1993, 9:119-131.
13. Londoño-Vallejo J.A., Dubnau D. Mutation of the putative nucleotide binding site of the Bacillus subtilis membrane protein ComFA abolishes the uptake of DNA during transformation. J. Bacteriol. 1994, 176:4642-4645.
14. Draskovic I., Dubnau D. Biogenesis of a putative channel protein, ComEC, required for DNA uptake: membrane topology, oligomerization and formation of disulphide bonds. Mol. Microbiol. 2005, 55:881-896.
15. Chen I., Dubnau D. DNA uptake during bacterial transformation. Nat. Rev. Microbiol. 2004, 2:241-249.
16. Kelly M.S., Pritchard R.H. Unstable linkage between genetic markers in transformation. J. Bacteriol. 1965, 89:1314-1321.
17. Bettinger G.E., Young F.E. Transformation of Bacillus subtilis using gently lysed L-forms: a new mapping technique. Biochem. Biophys. Res. Commun. 1973, 55:1105-1111.
18. Bettinger G.E., Young F.E. Transformation of Bacillus subtilis: transforming ability of deoxyribonucleic acid in lysates of L-forms or protoplasts. J. Bacteriol. 1975, 122:987-993.
19. Akamatsu T., Sekiguchi J. Characterization of chromosome and plasmid transformation in Bacillus subtilis using gently lysed protoplasts. Arch. Microbiol. 1987, 146:353-357.
20. Saito Y., Taguchi H., Akamatsu T. Fate of transforming bacterial genome following incorporation into competent cells of Bacillus subtilis: a continuous length of incorporated DNA. J. Biosci. Bioeng. 2006, 101:257-262.
21. Hahn J., Albano M., Dubnau D. Isolation and Characterization of Tn917lac-Generated Competence Mutants of Bacillus subtilis. J. Bacteriol. 1987, 169:3104-3109.
22. Yang L., Ukil L., Osmani A., Nahm F., Davies J., De Souza C.P., Dou X., Perez-Balaguer A., Osmani S.A. Rapid production of gene replacement constructs and generation of a green fluorescent protein-tagged centromeric marker in Aspergillus nidulans. Eukaryot. Cell 2004, 3:1359-1362.
23. Akamatsu T., Moriyama H., Kaneda T. Genetic mapping of bfmA mutation causing fatty acid deficiency in Bacillus subtilis. Can. J. Microbiol. 1993, 39:629-633.
24. Sekiguchi J., Ohsu H., Kuroda A., Moriyama H., Akamatsu T. Nucleotide sequences of the Bacillus subtilis flaD locus and a B. licheniformis homologue affecting the autolysin level and flagellation, J. Gen. Microbiol. 1990, 136:1223-1230.
25. Lacks S.A., Lopez P., Greenberg B., Espinosa M. Identification and analysis of genes for tetracycline resistance and replication functions in the broad-host-range plasmid pLS1. J. Mol. Biol. 1986, 192:753-765.
26. Akamatsu T., Taguchi H. Interspecific transformation of Bacillus subtilis competent cells by chromosomal DNA in lysates of protoplasts of Bacillus amyloliquefaciens. Biosci. Biotechnol. Biochem. 2000, 64:275-279.
27. Spizizen J. Transformation of biochemically deficient strains of Bacillus subtilis by deoxyribonucleate. Proc. Natl. Acad. Sci. USA 1958, 44:1072-1078.
28. Saito Y., Taguchi H., Akamatsu T. DNA taken into Bacillus subtilis competent cells by LP transformation is not ssDNA but dsDNA. J. Biosci. Bioeng. 2006, 101:334-339.
29. Akamatsu T., Taguchi H., Okada H. A simple and rapid extraction of high molecular weight chromosomal DNA from Bacillus subtilis protoplasts for cosmid cloning and interspecific transformation. Biosci. Biotechnol. Biochem. 2000, 64:1082-1083.
30. Birnboim H.C., Doly J. A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Res. 1979, 7:1513-1523.
31. Maniatis T., Fritsch E.F., Sambrook J. Molecular cloning, a laboratory manual 1982, Cold Spring Harbor Laboratory, Cold Spring Harbor, NY.
32. Kemper J. Gene order and co-transduction in the leu-ara-fol-pyrA region of the Salmonella typhimurium linkage map. J. Bacteriol. 1974, 117:94-99.
33. Rivolta C., Pagni M. Genetic and physical maps of the Bacillus subtilis chromosome. Genetics 1999, 151:1239-1244.
34. Mandecki W., Krajewska-Grynkiewicz K., Klopotowski T. A quantitative model for nonrandom generalized transduction, applied to the phage P22-Salmonella typhimurium system. Genetics 1986, 114:633-657.
35. Charles S.P., Garrett M.O., George F.O. Cellular motions and thermal fluctuations: the Brownian ratchet. Biophys. J. 1993, 65:316-324.
36. Sonia L.B., Sandy Y.M.N., Ken F.J. Prokaryotic motility structures. Microbiology 2003, 149:295-304.
37. Lindén M., Tuohimaa T., Jonsson A.B., Wallin M. Force generation in small ensembles of Brownian motors. Phys. Rev. E Stat. Nonlin. Soft. Matter. Phys. 2006, 74:021908.
38. Richard J.N., Nicholas G., Philip C. Lactose permease H1-lactose symporter: mechanical switch or Brownian ratchet?. Biophys. J. 2007, 92:3474-3491.
39. Kramer N., Hahn J., Dubnau D. Multiple interactions among the competence proteins of Bacillus subtilis. Mol. Microbiol. 2007, 65:454-464.