A simple and rapid extraction of high molecular weight chromosomal dna from bacillus subtilis protoplasts for cosmid cloning and interspecific transformation

Bioscience, Biotechnology and Biochemistry

Volumn 64, Issue 5, 2000, Pages 1082-1083

  Akamatsu, Takashi ^a   Taguchi, Hisataka ^a   Okada, Hirosuke ^a  

^a SOJO UNIVERSITY   (Japan)

Author keywords

Bacillus subtilis; DNA; Extraction; High molecular weight; transformation

Indexed keywords

BACILLUS SUBTILIS; BOVINAE;

BACTERIAL DNA; BOVINE SERUM ALBUMIN;

ANIMAL; ARTICLE; BACILLUS SUBTILIS; BACTERIUM TRANSFORMATION; CATTLE; COSMID; GENETICS; ISOLATION AND PURIFICATION; MOLECULAR CLONING; PROTOPLAST; PULSED FIELD GEL ELECTROPHORESIS;

ANIMALS; BACILLUS SUBTILIS; CATTLE; CLONING, MOLECULAR; COSMIDS; DNA, BACTERIAL; ELECTROPHORESIS, GEL, PULSED-FIELD; PROTOPLASTS; SERUM ALBUMIN, BOVINE; TRANSFORMATION, BACTERIAL;

EID: 0034180642     PISSN: 09168451     EISSN: 13476947     Source Type: Journal    
DOI: 10.1271/bbb.64.1082     Document Type: Article

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