High level expression and characterization of a novel thermostable, organic solvent tolerant, 1,3-regioselective lipase from Geobacillus sp. strain ARM

Bioresource Technology

Volumn 102, Issue 13, 2011, Pages 6972-6981

  Ebrahimpour, Afshin ^a   Rahman, Raja Noor Zaliha Raja Abd ^a   Basri, Mahiran ^a   Salleh, Abu Bakar ^a  

^a UNIVERSITY PUTRA MALAYSIA   (Malaysia)

Author keywords

1,3 Regioselective; Expression; Lipase; Organic solvent tolerant; Thermostable

Indexed keywords

1 ,3-SPECIFIC LIPASE; 1,3-REGIOSELECTIVE; DISULFIDE BONDS; EXPRESSION; EXPRESSION VECTORS; HIGH LEVEL EXPRESSION; IMMOBILIZED METAL AFFINITY CHROMATOGRAPHY; LIPASE ACTIVITY; LIPASE GENE; LIPOLYTIC ACTIVITY; METALLOENZYMES; ONE-STEP PURIFICATION; ORGANIC SOLVENT TOLERANT; RECOVERY AND PURIFICATIONS; RESIDUAL ACTIVITY; SOLVENT TOLERANT; THERMOSTABLE; WILD TYPES;

AFFINITY CHROMATOGRAPHY; AMINO ACIDS; COVALENT BONDS; ENZYMES; ESCHERICHIA COLI; METAL RECOVERY; ORGANIC COMPOUNDS; ORGANIC SOLVENTS; PURIFICATION; REGIOSELECTIVITY; SOLVENTS;

GENE EXPRESSION;

1,3 REGIOSELECTIVE LIPASE; BACTERIAL DNA; BACTERIAL ENZYME; CHELATING AGENT; METAL ION; ORGANIC SOLVENT; UNCLASSIFIED DRUG;

BACTERIUM; CHROMATOGRAPHY; ENZYME ACTIVITY; GENE EXPRESSION; IMMOBILIZATION; MOLECULAR ANALYSIS; PH; PURIFICATION; RECOMBINATION; SOLVENT; TEMPERATURE EFFECT; TOLERANCE;

ARTICLE; BACTERIAL GENE; BACTERIAL STRAIN; ENZYME ACTIVITY; GEOBACILLUS; IMMOBILIZED METAL AFFINITY CHROMATOGRAPHY; LIPOLYSIS; MOLECULAR WEIGHT; NONHUMAN; PH; PRIORITY JOURNAL; TEMPERATURE; THERMOSTABILITY;

ADAPTATION, PHYSIOLOGICAL; AMINO ACID SEQUENCE; BASE SEQUENCE; CLONING, MOLECULAR; ENZYME INHIBITORS; ENZYME STABILITY; GENE EXPRESSION REGULATION, BACTERIAL; GENES, BACTERIAL; GEOBACILLUS; HYDROGEN-ION CONCENTRATION; IONS; LIPASE; METALS; MOLECULAR SEQUENCE DATA; MOLECULAR WEIGHT; ORGANIC CHEMICALS; RECOMBINANT PROTEINS; SEQUENCE ALIGNMENT; SEQUENCE ANALYSIS, PROTEIN; SOLVENTS; STEREOISOMERISM; SUBSTRATE SPECIFICITY; TEMPERATURE;

ESCHERICHIA COLI; GEOBACILLUS;

EID: 79956356331     PISSN: 09608524     EISSN: 18732976     Source Type: Journal    
DOI: 10.1016/j.biortech.2011.03.083     Document Type: Article

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