A Simple Assay for Mammalian Spermine Oxidase: A Polyamine Catabolic Enzyme Implicated in Drug Response and Disease

Methods in Molecular Biology

Volumn 720, Issue , 2011, Pages 173-181

  Goodwin, Andrew C ^a   Murray Stewart, Tracy R ^a   Casero, Robert A ^b  

^a JOHNS HOPKINS UNIVERSITY   (United States)

^b JOHNS HOPKINS UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

Chemiluminescence; Hydrogen peroxide; Polyamine catabolism; Spermine oxidase

Indexed keywords

CELL EXTRACT; HYDROGEN PEROXIDE; OXIDOREDUCTASE; POLYAMINE; POLYAMINE OXIDASE;

ANIMAL; ARTICLE; CELL ADHESION; CELL SEPARATION; CHEMISTRY; ENZYME ASSAY; GENERAL ASPECTS OF DISEASE; MAMMAL; METABOLISM; METHODOLOGY; STANDARD;

ANIMALS; CELL ADHESION; CELL EXTRACTS; CELL SEPARATION; DISEASE; ENZYME ASSAYS; HYDROGEN PEROXIDE; MAMMALS; OXIDOREDUCTASES ACTING ON CH-NH GROUP DONORS; POLYAMINES; REFERENCE STANDARDS;

EID: 79956227428     PISSN: 10643745     EISSN: 19406029     Source Type: Book Series    
DOI: 10.1007/978-1-61779-034-8_10     Document Type: Chapter

References (17)

1. Casero RA, Pegg AE (2009) Polyamine catabolism and disease. Biochem J 421:323–338
2. Casero RA Jr, Pegg AE (1993) Spermidine/ spermine N1-acetyltransferase – the turning point in polyamine metabolism. FASEB J 7:653–661
3. Vujcic S, Liang P, Diegelman P, Kramer DL, Porter CW (2003) Genomic identification and biochemical characterization of the mammalian polyamine oxidase involved in polyamine back-conversion. Biochem J 370:19–28
4. Wu T, Yankovskaya V, McIntire WS (2003) Cloning, sequencing, and heterologous expression of the murine peroxisomal fla-voprotein, n1-acetylated polyamine oxidase. J Biol Chem 278:20514–20525
5. Wang Y, Devereux W, Woster P, Stewart T, Hacker A, Casero R Jr (2001) Cloning and characterization of a human polyamine oxidase that is inducible by polyamine analogue exposure. Cancer Res 61:5370–5373
6. Vujcic S, Diegelman P, Bacchi CJ, Kramer DL, Porter CW (2002) Identification and characterization of a novel flavin-containing spermine oxidase of mammalian cell origin. Biochem J 367:665–675
7. Chaturvedi R, Cheng Y, Asim M, Bussiere FI, Xu H, Gobert AP, Hacker A, Casero RA Jr, Wilson KT (2004) Induction of polyamine oxidase 1 by Helicobacter pylori causes macrophage apoptosis by hydrogen peroxide release and mitochondrial membrane depolar-ization. J Biol Chem 279:40161–40173
8. Xu H, Chaturvedi R, Cheng Y, Bussiere FI, Asim M, Yao MD, Potosky D, Meltzer SJ, Rhee JG, Kim SS, Moss SF, Hacker A, Wang Y, Casero RA Jr, Wilson KT (2004) Spermine oxidation induced by Helicobacter pylori results in apoptosis and DNA damage: implications for gastric carcinogenesis. Cancer Res 64:8521–8525
9. Goodwin AC, Jadallah S, Toubaji A, Lecksell K, Hicks JL, Kowalski J, Bova GS, De Marzo AM, Netto GJ, Casero RA Jr (2008) Increased spermine oxidase expression in human prostate cancer and prostatic intraepithelial neoplasia tissues. Prostate 68:766–772
10. Ha HC, Woster PM, Yager JD, Casero RA Jr (1997) The role of polyamine catabolism in polyamine analogue-induced programmed cell death. Proc Natl Acad Sci U S A 94:11557–11562
11. Chen Y, Kramer DL, Diegelman P, Vujcic S, Porter CW (2001) Apoptotic signaling in polyamine analogue-treated SK-MEL-28 human melanoma cells. Cancer Res 61:6437–6444
12. Devereux W, Wang Y, Stewart TM, Hacker A, Smith R, Frydman B, Valasinas AL, Reddy VK, Marton LJ, Ward TD, Woster PM, Casero RA (2003) Induction of the PAOh1/SMO polyamine oxidase by polyamine analogues in human lung carcinoma cells. Cancer Chemother Pharmacol 52:383–390
13. 2 in polyamine analogue-treated human breast cancer cell lines. J Biol Chem 280:39843–39851
14. Pledgie-Tracy A, Billam M, Hacker A, Sobolewski MD, Woster PM, Zhang Z, Casero RA, Davidson NE (2010) The role of the polyamine catabolic enzymes SSAT and SMO in the synergistic effects of standard chemotherapeutic agents with a polyamine analogue in human breast cancer cell lines. Cancer Chemother Pharmacol 65(6):1067–1081
15. Fernandez C, Sharrard RM, Talbot M, Reed BD, Monks N (1995) Evaluation of the significance of polyamines and their oxidases in the aetiology of human cervical carcinoma. Br J Cancer 72:1194–1199
16. Wang Y, Murray-Stewart T, Devereux W, Hacker A, Frydman B, Woster P, Casero R Jr (2003) Properties of purified recombinant human polyamine oxidase, PAOh1/SMO. Biochem Biophys Res Commun 304:605–611
17. Murray-Stewart T, Wang Y, Goodwin A, Hacker A, Meeker A, Casero RA Jr (2008) Nuclear localization of human spermine oxidase isoforms – possible implications in drug response and disease etiology. FEBS J 275:2795–2806