Long-term ex vivo monitoring of in vivo microRNA activity in liver using a secreted luciferase sensor

Science China Life Sciences

Volumn 54, Issue 5, 2011, Pages 418-425

  Wang, Gang ^a   Dong, Xiao Yan ^a,b,c   Hu, Jian Yang ^a,d   Tian, Wen Hong ^a   Yuchi, Jie ^a   Wang, Yue ^a   Wu, Xiao Bing ^a  

^a NATIONAL INSTITUTE FOR VIRAL DISEASE CONTROL AND PREVENTION   (China)

^b FUDAN UNIVERSITY   (China)

^c Beijing Fiveplus Molecular Medicine Institute   (China)

^d AFFILIATED HOSPITAL OF NINGXIA MEDICAL UNIVERSITY   (China)

Author keywords

ex vivo monitoring; microRNA sensor; secreted Gaussia luciferase

Indexed keywords

BIOLOGICAL MARKER; LUCIFERASE; MICRORNA; MIRN122 MICRORNA, HUMAN; MIRN143 MICRORNA, HUMAN; MIRN34 MICRORNA, HUMAN;

3' UNTRANSLATED REGION; ANIMAL; ARTICLE; BAGG ALBINO MOUSE; CRUSTACEA; GENE SILENCING; GENETICS; HELA CELL; HUMAN; LIVER; MALE; METABOLISM; MOUSE; PROMOTER REGION;

3' UNTRANSLATED REGIONS; ANIMALS; BIOLOGICAL MARKERS; CRUSTACEA; GENE SILENCING; HELA CELLS; HUMANS; LIVER; LUCIFERASES; MALE; MICE; MICE, INBRED BALB C; MICRORNAS; PROMOTER REGIONS, GENETIC;

GAUSSIA PRINCEPS (T. SCOTT, 1894);

EID: 79956147543     PISSN: 16747305     EISSN: None     Source Type: Journal    
DOI: 10.1007/s11427-011-4171-0     Document Type: Article

References (25)

1. Ambros V. The functions of animal microRNAs. Nature, 2004, 431: 350-355.
2. Wang Y, Kato N, Jazag A, et al. Hepatitis C virus core protein is a potent inhibitor of RNA silencing-based antiviral response. Gastroenterology, 2006, 130: 883-892.
3. Carthew R W, Sontheimer E J. Origins and mechanisms of miRNAs and siRNAs. Cell, 2009, 136: 642-655.
4. Kim V N. MicroRNA biogenesis: coordinated cropping and dicing. Nat Rev Mol Cell Biol, 2005, 6: 376-385.
5. Válóczi A, Hornyik C, Varga N, et al. Sensitive and specific detection of microRNAs by Northern blot analysis using LNA-modified oligonucleotide probes. Nucleic Acids Res, 2004, 32: e175.
6. Chen J, Lozach J, Garcia E W, et al. Highly sensitive and specific microRNA expression profiling using BeadArray technology. Nucleic Acids Res, 2008, 36: e87.
7. Mestdagh P, Feys T, Bernard N, et al. High-throughput stem-loop RT-qPCR miRNA expression profiling using minute amounts of input RNA. Nucleic Acids Res, 2008, 36: e143.
8. Mansfield J H, Harfe B D, Nissen R, et al. MicroRNA-responsive 'sensor' transgenes uncover Hox-like and other developmentally regulated patterns of vertebrate microRNA expression. Nat Genet, 2004, 36: 1079-1083.
9. Brown B D, Gentner B, Cantore A, et al. Endogenous microRNA can be broadly exploited to regulate transgene expression according to tissue, lineage and differentiation state. Nat Biotechnol, 2007, 25: 1457-1467.
10. Lee J Y, Kim S, Hwang do W, et al. Development of a dualluciferase reporter system for in vivo visualization of MicroRNA biogenesis and posttranscriptional regulation. J Nucl Med, 2008, 49: 285-294.
11. Kim H J, Chung J K, Hwang D W, et al. In vivo imaging of miR-221 biogenesis in papillary thyroid carcinoma. Mol Imaging Biol, 2009, 11: 71-78.
12. Barth S, Pfuhl T, Mamiani A, et al. Epstein-Barr virus-encoded microRNA miR-BART2 down-regulates the viral DNA polymerase BALF5. Nucleic Acids Res, 2008, 36: 666-675.
13. Wurdinger T, Badr C, Pike L, et al. A secreted luciferase for ex vivo monitoring of in vivo processes. Nat Methods, 2008, 5: 171-173.
14. Chung E, Yamashita H, Au P, et al. Secreted Gaussia luciferase as a biomarker for monitoring tumor progression and treatment response of systemic metastases. PLoS One, 2009, 4: e8316.
15. Tannous B A. Gaussia luciferase reporter assay for monitoring biological processes in culture and in vivo. Nat Protoc, 2009, 4: 582-591.
16. Liu F, Song Y, Liu D. Hydrodynamics-based transfection in animals by systemic administration of plasmid DNA. Gene Ther, 1999, 6: 1258-1266.
17. Jopling C L, Yi M, Lancaster A M, et al. Modulation of hepatitis C virus RNA abundance by a liver-specific MicroRNA. Science, 2005, 309: 1577-1581.
18. Lagos-Quintana M, Rauhut R, Yalcin A, et al. Identification of tissue-specific microRNAs from mouse. Curr Biol, 2002, 12: 735-739.
19. Brown B D, Cantore A, Annoni A, et al. A microRNA-regulated lentiviral vector mediates stable correction of hemophilia B mice. Blood, 2007, 110: 4144-4152.
20. Zhang B, Pan X. RDX induces aberrant expression of microRNAs in mouse brain and liver. Environ Health Perspect, 2009, 117: 231-240.
21. Al-Dosari M, Zhang G, Knapp J E, et al. Evaluation of viral and mammalian promoters for driving transgene expression in mouse liver. Biochem Biophys Res Commun, 2006, 339: 673-678.
22. Nguyen A T, Dow A C, Kupiec-Weglinski J, et al. Evaluation of gene promoters for liver expression by hydrodynamic gene transfer. Surg Res, 2008, 148: 60-66.
23. Suzuki T, Sakurai F, Nakamura S, et al. miR-122a-regulated expression of a suicide gene prevents hepatotoxicity without altering antitumor effects in suicide gene therapy. Mol Ther, 2008, 16: 1719-1726.
24. Wolff L J, Wolff J A, Sebestyén M G. Effect of tissue-specific promoters and microRNA recognition elements on stability of transgene expression after hydrodynamic naked plasmid DNA delivery. Hum Gene Ther, 2009, 20: 374-388.
25. Cawood R, Chen H H, Carroll F, et al. Use of tissue-specific microRNA to control pathology of wild-type adenovirus without attenuation of its ability to kill cancer cells. PLoS Pathog, 2009, 5: e1000440.