Measurements of Endosomal pH in Live Cells by Dual-Excitation Fluorescence Imaging

Cell Biology, Four-Volume Set

Volumn 3, Issue , 2006, Pages 163-169

  Demaurex, Nicolas ^a   Grinstein, Sergio ^b  

^a UNIVERSITY OF GENEVA MEDICAL SCHOOL   (Switzerland)

^b HOSPITAL FOR SICK CHILDREN   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

EID: 79956090209     PISSN: None     EISSN: None     Source Type: Book    
DOI: 10.1016/B978-012164730-8/50144-1     Document Type: Chapter

References (7)

1. Demaurex N. pH Homeostasis of cellular organelles. News Physiol Sci. 2002, 17:1-5.
2. Demaurex N., Furuya W., D'Souza S., Bonifacino J.S., Grinstein S. Mechanism of acidification of the trans-Golgi network (TGN): In situ measurements of pH using retrieval of TGN38 and furin from the cell surface. J. Biol. Chem. 1998, 273(4):2044-2051.
3. Gagescu R., Demaurex N., Parton R.G., Hunziker W., Huber L.A., Gruenberg J. The recycling endosome of Madin-Darby canine kidney cells is a mildly acidic compartment rich in raft components. Mol. Biol. Cell. 2000, 11(8):2775-2791.
4. Grinstein S., Furuya W. Assessment of Na+-H+ exchange activity in phagosomal membranes of human neutrophils. Am. J. Physiol. 1988, 254(2 Pt 1):C272-C285.
5. Piguet V., Gu F., Foti M., Demaurex N., Gruenberg J., Carpentier J.L., Trono D. Nef-induced CD4 degradation: A diacidic-based motif in Nef functions as a lysosomal targeting signal through the binding of beta-COP in endosomes. Cell 1999, 97(1):63-73.
6. Piguet V., Wan L., Borel C., Mangasarian A., Demaurex N., Thomas G., Trono D. HIV-1 Nef protein binds to the cellular protein PACS-1 to downregulate class I major histocompatibility complexes. Nature Cell Biol. 2000, 2(3):163-167.
7. Thomas J.A., Buchsbaum R.N., Zimniak A., Racker E. Intracellular pH measurements in Ehrlich ascites tumor cells utilizing spectroscopic probes generated in situ. Biochemistry 1979, 18(11):2210-2218.