Non-invasive genotyping of transgenic mice: Comparison of different commercial kits and required amounts

Altex

Volumn 27, Issue 3, 2010, Pages 185-190

  Hamann, Melanie ^a   Lange, Nikola ^a   Kuschka, Jagoda ^a   Richter, Angelika ^a  

^a FREIE UNIVERSITÄT BERLIN   (Germany)

Author keywords

Faecal samples; Genetically modified animals; Genotyping; Stool samples; Tail biopsy

Indexed keywords

DNA;

ANIMAL EXPERIMENT; ANIMAL TISSUE; ANIMAL WELFARE; ARTICLE; CONTROLLED STUDY; DNA EXTRACTION; DNA ISOLATION; DNA MODIFICATION; FECES; GENOTYPE; LABORATORY APPARATUS, EQUIPMENT AND SUPPLIES; MOUSE; NONHUMAN; TRANSGENIC MOUSE; ANALYTICAL EQUIPMENT; ANIMAL; CHEMISTRY;

ANIMALS; DNA; FECES; GENOTYPE; MICE; MICE, TRANSGENIC; REAGENT KITS, DIAGNOSTIC;

EID: 79955962790     PISSN: 09467785     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (19)

1. Arras, M., Rettich, A., Seifert, B. et al. (2007). Should laboratory mice be anaesthetized for tail biopsy? Lab. Anim. 41, 30-45. (Pubitemid 46174425)
2. Breakefield, X. O., Blood, A. J., Li. Y. et al. (2008). The pathophysiological basis of dystonia. Nat. Rev. Neurosci. 9, 222-234.
3. Broome, R. L., Feng, L., Zhou, Q. et al. (1999). Non-invasive mouse genotyping using stool analysis. FEBS Letters 462, 159-160.
4. Buehr, M., Hjorth, J. P., Hansen, A. K. et al. (2003). Genetically modified laboratory animals - what welfare problems do they face? J. Appl. Anim. Welf. Sci. 6(4), 319-338. (Pubitemid 39172265)
5. Chang, W. W. L. and Leblond, C. P. (1971). Renewal of the epithelium in the descending colon of the mouse. I. Presence of three cell populations: vacuolated-columnar, mucous and argentaffin. Am. J. Anat. 131, 73-99.
6. Irwin, M. H., Moffatt, J. and Pinkert, C. A. (1996). Identification of transgenic mice by PCR analysis of saliva. Nature Biotechnol. 14, 1146-1148. (Pubitemid 26299117)
7. Jennings, M., Batchelor, G. R., Brain, P. F. et al. (1998). Refining rodent husbandry: the mouse. Lab. Anim. 32, 233-259. (Pubitemid 28379164)
8. Kalippke, K., Werwitzke, S., Von Hornung, M. et al. (2009). DNA analysis from stool samples: a fast and reliable method avoiding invasive sampling methods in mouse models of bleeding disorders. Lab. Anim. 43, 390-393.
9. Murgatroyd, C., Bilko, D. and Spengler, D. (2006). Isolation of high-quality DNA for genotyping from faeces of rodents. Anal. Biochem. 348, 160-162. (Pubitemid 41796472)
10. Nicklas, W., Baneux, P., Boot, R. et al. (2002). Recommendations for the health monitoring of rodent and rabbit colonies in breeding and experimental units. Lab. Anim. 36, 20-42. (Pubitemid 34073119)
11. Pinkert, C. A. (2003). Transgenic animal technology: alternatives in genotyping and phenotyping. Comp. Med. 53 (2), 126-139.
12. Robinson, V., Morton, D. B., Anderson, D. et al. (2003). Refinement and reduction in production of genetically modified mice. Lab. Anim. 37, Suppl. 1, S1: 27-33.
13. Sauer, U. G., Kolar, R. and Rusche B. (2005). The use of transgenic animals in biomedical research in Germany. Part 1: Status Report 2001-2003. ALTEX 22 (4), 233-257.
14. Sauer, U. G., Kolar, R. and Rusche B. (2006). The use of transgenic animals in biomedical research in Germany. Part 2: Ethical evaluation of the use of transgenic animals in biomedical research and perspectives for the changeover in research to research animal-free methods. ALTEX 23 (1), 3-16.
15. Schneider, M. R. and Wolf, E. (2005). Genotyping of transgenic mice: Old principles and recent developments. Anal. Biochem. 344, 1-7. (Pubitemid 41569371)
16. Schubbert, R., Lettman, C. and Doerfler, W. (1994). Ingested foreign (phage M13) DNA survives transiently in the gastrointestinal tract and enters the bloodstream of mice. Mol. Gen. Genet. 242, 495-504. (Pubitemid 24086889)
17. Shashidharan, P., Sandu, D., Potla, U. et al. (2005). Transgenic mouse model of early-onset DYT1 dystonia. Hum. Mol. Genet. 14 (1), 125-133. (Pubitemid 40123468)
18. Tanabe, L. M., Kim, C. E., Alagem, N. et al. (2009). Primary dystonia: molecules and mechanisms. Nat. Rev. Neurol. 5, 598-609.
19. Truett, G. E., Heeger, P., Mynatt, R. L. et al. (2000). Preparation of PCR-quality mouse genomic DNA with hot sodium hydroxide and tris (HotSHOT). Biotechniques 29, 52-54. (Pubitemid 30439416)