Preparation of PCR-quality mouse genomic dna with hot sodium hydroxide and tris (HotSHOT)

BioTechniques

Volumn 29, Issue 1, 2000, Pages 52-54

  Truett, Gary E ^a,c   Heeger, P ^b   Mynatt, R L ^a   Truett, A A ^a   Walker, J A ^a   Warman, M L ^b  

^a Center   (United States)

^b CASE WESTERN RESERVE UNIVERSITY   (United States)

^c PENNINGTON BIOMEDICAL RESEARCH CENTER   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

SODIUM HYDROXIDE;

ARTICLE; CLINICAL PROTOCOL; DNA DETERMINATION; DNA PURIFICATION; GENE AMPLIFICATION; NONHUMAN; POLYMERASE CHAIN REACTION;

EID: 0033922371     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/00291bm09     Document Type: Article

References (3)

1. Cesarone, C., C. Bolognesi, and L. Santi. 1979. Improved microflurometric DNA determination in biological material using 33258 Hoechst. Anal. Biochem. 102:188-197.
2. Rudbeck, L. and J. Dissing. 1998. Rapid, simple alkaline extraction of human genomic DNA from whole blood, buccal epithelial cells, semen and forensic stains for PCR. BioTechniques 25:588-592.
3. Strauss, W.M. 1998. Preparation of genomic DNA from mammalian tissue, p. 2.2.1-2.2.3. In Ausubel et al. (Eds.), Current Protocols in Molecular Biology. John Wiley & Sons, New York.