Enhancement of xylitol production by attenuation of intracellular xylitol dehydrogenase activity in Candida tropicalis

Biotechnology Letters

Volumn 33, Issue 6, 2011, Pages 1209-1213

  Ko, Byoung Sam ^a   Kim, Dong Min ^a   Yoon, Byoung Hoon ^b   Bai, Suk ^a   Lee, Hyeon Yong ^c   Kim, Jung Hoe ^b   Kim, Il Chul ^a  

^a Chonnam National University   (South Korea)

^b Korea Advanced Institute of Science and Technology (KAIST)   (South Korea)

^c Kangwon National University   (South Korea)

Author keywords

Candida tropicalis; Enzyme engineering; Fermentation; Xylitol; Xylitol dehydrogenase

Indexed keywords

CANDIDA TROPICALIS; COSUBSTRATES; DEHYDROGENASE ACTIVITY; ENZYME ENGINEERING; HIGH PRODUCTIVITY; HIGH YIELD; MUTANT STRAIN; WILD TYPES; XYLITOL; XYLITOL DEHYDROGENASE;

CANDIDA; GENES; GLYCEROL; PRODUCTIVITY; SUBSTRATES; YEAST;

SUGAR SUBSTITUTES;

FUNGAL DNA; FUNGAL PROTEIN; MUTANT PROTEIN; RECOMBINANT PROTEIN; XYLITOL; XYLOSE; XYLULOSE REDUCTASE;

ARTICLE; BIOSYNTHESIS; BIOTECHNOLOGY; CANDIDA TROPICALIS; FERMENTATION; FUNGAL GENE; GENETICS; KINETICS; METABOLISM; MUTATION; NUCLEOTIDE SEQUENCE; PROTEIN ENGINEERING; SITE DIRECTED MUTAGENESIS;

BASE SEQUENCE; BIOTECHNOLOGY; CANDIDA TROPICALIS; D-XYLULOSE REDUCTASE; DNA, FUNGAL; FERMENTATION; FUNGAL PROTEINS; GENES, FUNGAL; KINETICS; MUTAGENESIS, SITE-DIRECTED; MUTANT PROTEINS; MUTATION; PROTEIN ENGINEERING; RECOMBINANT PROTEINS; XYLITOL; XYLOSE;

CANDIDA TROPICALIS;

EID: 79955942312     PISSN: 01415492     EISSN: 15736776     Source Type: Journal    
DOI: 10.1007/s10529-011-0558-z     Document Type: Article

References (11)

1. Alexander MA, Chapman TW, Jeffries TW (1988) Xylose metabolism by Candida shehatae in continuous culture. Appl Microbiol Biotechnol 29: 478-486.
2. Barbosa MFS, de Medeiros MB, de Mandilha IM, Schneider H, Lee H (1988) Screening of yeasts for production of xylitol from d-xylose and some factors which alter xylitol yield in Candida guilliermondii. J Ind Microbiol 3: 241-251.
3. Ito H, Fukuda Y, Murata K, Kimura J (1983) Transformation of intact yeast cells treated with alkali cations. J Bacteriol 153: 163-168.
4. Kim SY, Kim JH, Oh DK (1997) Improvement of xylitol production by controlling oxygen supply in Candida parapsilosis. J Ferment Bioeng 83: 267-270.
5. Kim SY, Kim JH, Oh DK (1998) Effect of redox potential on xylitol production by Candida tropicalis. Food Sci Biotechnol 7: 282-285.
6. +-dependent xylitol dehydrogenase from Candida tropicalis ATCC 20913. Biotechnol Prog 22: 1708-1714.
7. Ko BS, Kim J, Kim JH (2006b) Production of xylitol from d-xylose by a xylitol dehydrogenase gene-disrupted mutant of Candida tropicalis. Appl Environ Microbiol 72: 4207-4213.
8. Ko BS, Rhee CH, Kim JH (2006c) Enhancement of xylitol productivity and yield using a xylitol dehydrogenase gene-disrupted mutant of Candida tropicalis under fully aerobic conditions. Biotechnol Lett 28: 1159-1162.
9. Mäkinen KK (1992) Dietary prevention of dental caries by xylitol-clinical effectiveness and safety. J Appl Nutr 44: 16-28.
10. Penning TM, Jez JM (2001) Enzyme redesign. Chem Rev 101: 3027-3046.
11. Watanabe S, Kodaki T, Makino K (2005) Complete reversal of coenzyme specificity of xylitol dehydrogenase and increase of thermostability by the introduction of structural zinc. J Biol Chem 280: 10340-10349.