Over-expression of peptide deformylase in chloroplasts confers actinonin resistance, but is not a suitable selective marker system for plastid transformation

Transgenic Research

Volumn 20, Issue 3, 2011, Pages 613-624

  Millán, Alicia Fernández San ^a   Obregón, Patricia ^a   Veramendi, Jon ^a  

^a PUBLIC UNIVERSITY OF NAVARRE   (Spain)

Author keywords

Deformylase; Plastid transformation; Selective marker; Tobacco

Indexed keywords

ACTINONIN; AMIDASE; HYDROXAMIC ACID; PEPTIDE DEFORMYLASE;

ARABIDOPSIS; ARTICLE; CHLOROPLAST; DRUG EFFECT; DRUG RESISTANCE; ENZYMOLOGY; EVALUATION; GENETIC MARKER; GENETIC TRANSFORMATION; GENETICS; METABOLISM; TOBACCO; TRANSGENIC PLANT; UPREGULATION;

AMIDOHYDROLASES; ARABIDOPSIS; CHLOROPLASTS; DRUG RESISTANCE; GENETIC MARKERS; HYDROXAMIC ACIDS; PLANTS, GENETICALLY MODIFIED; TOBACCO; TRANSFORMATION, GENETIC; UP-REGULATION;

ARABIDOPSIS; ARABIDOPSIS THALIANA; NICOTIANA TABACUM;

EID: 79955817164     PISSN: 09628819     EISSN: None     Source Type: Journal    
DOI: 10.1007/s11248-010-9447-9     Document Type: Article

References (33)

1. Barone P, Zhang XH, Widholm JM (2009) Tobacco plastid transformation using the feedback-insensitive anthranilate synthase [alpha]-subunit of tobacco (ASA2) as a new selectable marker. J Exp Bot 60: 3195-3202.
2. Carrer H, Hockenberry TN, Svab Z, Maliga P (1993) Kanamycin resistance as a selectable marker for plastid transformation in tobacco. Mol Gen Genet 241: 49-56.
3. Chen DZ, Patel DV, Hackbarth CJ, Wang W, Dreyer G, Young DC, Margolis PS, Wu C, Ni ZJ, Trias J, White RJ, Yuan Z (2000) Actinonin, a naturally occurring antibacterial agent, is a potent deformylase inhibitor. Biochemistry 39: 1256-1262.
4. Daniell H, Khan MS, Allison L (2002) Milestones in chloroplast genetic engineering: an environmentally friendly era in biotechnology. Trends Plant Sci 7: 84-91.
5. Dirk LM, Williams MA, Houtz RL (2001) Eukaryotic peptide deformylases. Nuclear-encoded and chloroplast-targeted enzymes in Arabidopsis. Plant Physiol 127: 97-107.
6. Dirk LM, Schmidt JJ, Cai Y, Barnes JC, Hanger KM, Nayak NR, Williams MA, Grossman RB, Houtz RL, Rodgers DW (2008) Insights into the substrate specificity of plant peptide deformylase, an essential enzyme with potential for the development of novel biotechnology applications in agriculture. Biochem J 413: 417-427.
7. Farran I, Rio-Manterola F, Iniguez M, Garate S, Prieto J, Mingo-Castel AM (2008) High-density seedling expression system for the production of bioactive human cardiotrophin-1, a potential therapeutic cytokine, in transgenic tobacco chloroplasts. Plant Biotech J 6: 516-527.
8. Fernandez-San Millan A, Farran I, Molina A, Mingo-Castel AM, Veramendi J (2007) Expression of recombinant proteins lacking methionine as N-terminal amino acid in plastids: human serum albumin as a case study. J Biotechnol 127: 593-604.
9. Fernandez-San Millan A, Ortigosa SM, Hervas-Stubbs S, Corral-Martinez P, Segui-Simarro JM, Gaetan J, Coursaget P, Veramendi J (2008) Human papillomavirus L1 protein expressed in tobacco chloroplasts self-assembles into virus-like particles that are highly immunogenic. Plant Biotech J 6: 427-441.
10. Fernández-San Millán A, Mingo-Castel AM, Miller M, Daniell H (2003) A chloroplast transgenic approach to hyper-express and purify human serum albumin, a protein susceptible to proteolytic degradation. Plant Biotech J 1: 71-79.
11. Fromm H, Edelman M, Aviv D, Galun E (1987) The molecular basis for rRNA-dependent spectinomycin resistance in nicotiana chloroplasts. EMBO J 6: 3233-3237.
12. Giglione C, Meinnel T (2001) Organellar peptide deformylases: universality of the N-terminal methionine cleavage mechanism. Trends Plant Sci 6: 566-572.
13. Giglione C, Serero A, Pierre M, Boisson B, Meinnel T (2000) Identification of eukaryotic peptide deformylases reveals universality of N-terminal protein processing mechanisms. EMBO J 19: 5916-5929.
14. Giglione C, Vallon O, Meinnel T (2003) Control of protein life-span by N-terminal methionine excision. EMBO J 22: 13-23.
15. Haas M, Beyer D, Gahlmann R, Freiberg C (2001) YkrB is the main peptide deformylase in Bacillus subtilis, a eubacterium containing two functional peptide deformylases. Microbiology 147: 1783-1791.
16. Hou C-X, Dirk LMA, Williams MA (2004) Inhibition of peptide deformylase in nicotiana tabacum leads to decreased D1 protein accumulation, ultimately resulting in a reduction of photosystem II complexes. Am J Bot 91: 1304-1311.
17. Hou C-X, Dirk LMA, Goodman JP, Williams MA (2006) Metabolism of the peptide deformylase inhibitor actinonin in tobacco. Weed Sci 54: 246-254.
18. Hou CX, Dirk LM, Pattanaik S, Das NC, Maiti IB, Houtz RL, Williams MA (2007) Plant peptide deformylase: a novel selectable marker and herbicide target based on essential cotranslational chloroplast protein processing. Plant Biotechnol J 5: 275-281.
19. Huang FC, Klaus SM, Herz S, Zou Z, Koop HU, Golds TJ (2002) Efficient plastid transformation in tobacco using the aphA-6 gene and kanamycin selection. Mol Genet Genomics 268: 19-27.
20. Kumar S, Dhingra A, Daniell H (2004) Stable transformation of the cotton plastid genome and maternal inheritance of transgenes. Plant Mol Biol 56: 203-216.
21. Lee SB, Kwon HB, Kwon SJ, Park SC, Jeong MJ, Han SE, Byun MO, Daniell H (2003) Accumulation of trehalose within transgenic chloroplasts confers drought tolerance. Mol Breed 11: 1-13.
22. Li W, Ruf S and Bock R (2010) Chloramphenicol acetyltransferase as selectable marker for plastid transformation. Plant Mol Biol doi: 10. 1007/s11103-11010-19678-11104.
23. Lutz KA, Knapp JE, Maliga P (2001) Expression of bar in the plastid genome confers herbicide resistance. Plant Physiol 125: 1585-1590.
24. Maliga P (2004) Plastid transformation in higher plants. Annu Rev Plant Biol 55: 289-313.
25. Mazel D, Pochet S, Marliere P (1994) Genetic characterization of polypeptide deformylase, a distinctive enzyme of eubacterial translation. EMBO J 13: 914-923.
26. Meinnel T, Blanquet S (1994) Characterization of the thermus thermophilus locus encoding peptide deformylase and methionyl-tRNA(fMet) formyltransferase. J Bacteriol 176: 7387-7390.
27. Miki B, McHugh S (2004) Selectable marker genes in transgenic plants: applications, alternatives and biosafety. J Biotechnol 107: 193-232.
28. Molina A, Hervas-Stubbs S, Daniell H, Mingo-Castel AM, Veramendi J (2004) High-yield expression of a viral peptide animal vaccine in transgenic tobacco chloroplasts. Plant Biotech J 2: 141-153.
29. Moon S, Giglione C, Lee DY, An S, Jeong DH, Meinnel T, An G (2008) Rice peptide deformylase PDF1B is crucial for development of chloroplasts. Plant Cell Physiol 49: 1536-1546.
30. Murray SL, Thompson WF (1980) Rapid isolation of high molecular weight plant DNA. Nucleic Acids Res 8: 4321-4326.
31. Serero A, Giglione C, Meinnel T (2001) Distinctive features of the two classes of eukaryotic peptide deformylases. J Mol Biol 314: 695-708.
32. Svab Z, Maliga P (1993) High-frequency plastid transformation in tobacco by selection for a chimeric aadA gene. Proc Natl Acad Sci U S A 90: 913-917.
33. Ye GN, Colburn SM, Xu CW, Hajdukiewicz PT, Staub JM (2003) Persistence of unselected transgenic DNA during a plastid transformation and segregation approach to herbicide resistance. Plant Physiol 133: 402-410.