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Volumn 164, Issue 4, 2011, Pages 454-463

Investigating the structural and functional effects of mutating Asn glycosylation sites of Horseradish peroxidase to Asp

Author keywords

Glycosylation site; H2O2 inactivation; Protein stability; Recombinant horseradish peroxidase; Site directed mutagenesis

Indexed keywords

ACRYLAMIDES; ACTIVE SITE; ASPARAGINE RESIDUE; ASPARTIC ACIDS; BIOCATALYSIS; CATALYTIC ACTIVITY; CONFORMATIONAL CHANGE; DOUBLE MUTATION; H2O2 INACTIVATION; HORSERADISH PEROXIDASE; HYDROPHOBIC PATCH; MUTANT PROTEINS; NON-ADDITIVE; PROTEIN STABILITY; RECOMBINANT HORSERADISH PEROXIDASE; SITE DIRECTED MUTAGENESIS; THERMAL INACTIVATION;

EID: 79955807332     PISSN: 02732289     EISSN: None     Source Type: Journal    
DOI: 10.1007/s12010-010-9147-1     Document Type: Article
Times cited : (19)

References (47)
  • 1
    • 23044476987 scopus 로고    scopus 로고
    • Genetically engineered horseradish peroxidase for facilitated purification from baculovirus cultures by cation-exchange chromatography
    • DOI 10.1016/j.jbiotec.2005.05.015, PII S0168165605002403
    • G Levin F Mendive HM Targovnik C Osvaldo VM María 2005 Journal of Biotechnology 118 363 369 10.1016/j.jbiotec.2005.05.015 1:CAS:528: DC%2BD2MXmvVCqsLk%3D (Pubitemid 41058652)
    • (2005) Journal of Biotechnology , vol.118 , Issue.4 , pp. 363-369
    • Levin, G.1    Mendive, F.2    Targovnik, H.M.3    Cascone, O.4    Miranda, M.V.5
  • 2
    • 0031658804 scopus 로고    scopus 로고
    • Heterogeneity of glycans at each N-glycosylation site of horseradish peroxidase
    • DOI 10.1016/S0008-6215(98)00209-2, PII S0008621598002092
    • JS Gray B Yun Yang R Montgomery 1998 Carbohydrate Research 311 61 69 10.1016/S0008-6215(98)00209-2 1:CAS:528:DyaK1cXmvFyjsrw%3D (Pubitemid 28461911)
    • (1998) Carbohydrate Research , vol.311 , Issue.1-2 , pp. 61-69
    • Gray, J.S.S.1    Yang, B.Y.2    Montgomery, R.3
  • 3
    • 0018488111 scopus 로고
    • 10.1111/j.1432-1033.1979.tb13061.x 1:CAS:528:DyaE1MXlsFemsrc%3D
    • KG Welinder 1979 European Journal of Biochemistry 96 483 502 10.1111/j.1432-1033.1979.tb13061.x 1:CAS:528:DyaE1MXlsFemsrc%3D
    • (1979) European Journal of Biochemistry , vol.96 , pp. 483-502
    • Welinder, K.G.1
  • 5
    • 0002154654 scopus 로고
    • J. Everse K.E. Everse M.B. Grisham (eds). CRC Boca Raton
    • Dunford, H. B. (1991). In J. Everse, K. E. Everse, & M. B. Grisham (Eds.), Peroxidases in chemistry and biology, vol. 2 (pp. 1-24). Boca Raton: CRC.
    • (1991) Peroxidases in Chemistry and Biology, Vol. 2 , pp. 1-24
    • Dunford, H.B.1
  • 7
    • 0033121058 scopus 로고    scopus 로고
    • Recent biotechnological developments in the use of peroxidases
    • DOI 10.1016/S0167-7799(98)01288-8, PII S0167779998012888
    • S Colonna N Gaggero C Richelmi P Pasta 1999 Trends in Biotechnology 17 163 168 10.1016/S0167-7799(98)01288-8 1:CAS:528:DyaK1MXlt1KjtLk%3D (Pubitemid 29273947)
    • (1999) Trends in Biotechnology , vol.17 , Issue.4 , pp. 163-168
    • Colonna, S.1    Gaggero, N.2    Richelmi, C.3    Pasta, P.4
  • 10
    • 0034842869 scopus 로고    scopus 로고
    • Functional expression and stabilization of horseradish peroxidase by directed evolution in Saccharomyces cerevisiae
    • DOI 10.1002/bit.1149
    • B Morawski S Quan FH Arnold 2001 Biotechnology and Bioengineering 76 99 107 10.1002/bit.1149 1:CAS:528:DC%2BD3MXms1agtbs%3D (Pubitemid 32852484)
    • (2001) Biotechnology and Bioengineering , vol.76 , Issue.2 , pp. 99-107
    • Langsch, A.1    Bader, A.2
  • 11
    • 0032536139 scopus 로고    scopus 로고
    • 10.1016/S0014-5793(97)01573-1
    • JW Tams KG Welinder 1999 FEBS Letters 421 234 236 10.1016/S0014-5793(97) 01573-1
    • (1999) FEBS Letters , vol.421 , pp. 234-236
    • Tams, J.W.1    Welinder, K.G.2
  • 12
    • 34447094807 scopus 로고    scopus 로고
    • Effects of single mutations on the stability of horseradish peroxidase to hydrogen peroxide
    • DOI 10.1016/j.biochi.2007.03.013, PII S0300908407000776
    • BJ Ryan C O'Fagain 2007 Biochimie 89 1029 1032 10.1016/j.biochi.2007.03. 013 1:CAS:528:DC%2BD2sXnslWms78%3D (Pubitemid 47031432)
    • (2007) Biochimie , vol.89 , Issue.8 , pp. 1029-1032
    • Ryan, B.J.1    O'Fagain, C.2
  • 13
    • 33745932472 scopus 로고    scopus 로고
    • Horseradish and soybean peroxidases: Comparable tools for alternative niches?
    • DOI 10.1016/j.tibtech.2006.06.007, PII S0167779906001557
    • BJ Ryan N Carolan C O'Fágáin 2006 Trends in Biotechnology 24 355 363 10.1016/j.tibtech.2006.06.007 1:CAS:528:DC%2BD28XntVymtLs%3D (Pubitemid 44056266)
    • (2006) Trends in Biotechnology , vol.24 , Issue.8 , pp. 355-363
    • Ryan, B.J.1    Carolan, N.2    O'Fagain, C.3
  • 14
    • 47749085447 scopus 로고    scopus 로고
    • 10.1016/j.biochi.2008.05.008 1:CAS:528:DC%2BD1cXptVajtbo%3D
    • BJ Ryan C O'Fagain 2008 Biochimie 90 9 1414 1421 10.1016/j.biochi.2008. 05.008 1:CAS:528:DC%2BD1cXptVajtbo%3D
    • (2008) Biochimie , vol.90 , Issue.9 , pp. 1414-1421
    • Ryan, B.J.1    O'Fagain, C.2
  • 15
    • 0343471377 scopus 로고    scopus 로고
    • 1:CAS:528:DyaK2sXmslWnsb8%3D
    • CL Fisher GK Pei 1997 Biotechniques 23 570 574 1:CAS:528: DyaK2sXmslWnsb8%3D
    • (1997) Biotechniques , vol.23 , pp. 570-574
    • Fisher, C.L.1    Pei, G.K.2
  • 16
    • 0028964420 scopus 로고
    • 10.1016/0959-440X(95)80013-Q 1:CAS:528:DyaK2MXkt1Gqtr8%3D
    • RB Freedman 1995 Current Opinion in Structural Biology 5 85 91 10.1016/0959-440X(95)80013-Q 1:CAS:528:DyaK2MXkt1Gqtr8%3D
    • (1995) Current Opinion in Structural Biology , vol.5 , pp. 85-91
    • Freedman, R.B.1
  • 17
    • 0027997012 scopus 로고
    • 10.1006/jmbi.1994.1615 1:STN:280:DyaK2M%2FhvVagsA%3D%3D
    • C Wulfing A Plucthun 1994 Journal of Molecular Biology 242 655 669 10.1006/jmbi.1994.1615 1:STN:280:DyaK2M%2FhvVagsA%3D%3D
    • (1994) Journal of Molecular Biology , vol.242 , pp. 655-669
    • Wulfing, C.1    Plucthun, A.2
  • 18
    • 0030269967 scopus 로고    scopus 로고
    • Development of a simple method for the recovery of recombinant proteins from the Escherichia coli periplasm
    • DOI 10.1016/S0141-0229(96)00003-8
    • C French E Keshavarz-Moore JM Ward 1996 Enzyme and Microbial Technology 19 332 338 10.1016/S0141-0229(96)00003-8 1:CAS:528:DyaK28XlsFKktrs%3D (Pubitemid 26319491)
    • (1996) Enzyme and Microbial Technology , vol.19 , Issue.5 , pp. 332-338
    • French, C.1    Keshavarz-Moore, E.2    Ward, J.M.3
  • 20
    • 0014949207 scopus 로고
    • 10.1038/227680a0 1:CAS:528:DC%2BD3MXlsFags7s%3D
    • UK Laemmli 1970 Nature 227 680 685 10.1038/227680a0 1:CAS:528: DC%2BD3MXlsFags7s%3D
    • (1970) Nature , vol.227 , pp. 680-685
    • Laemmli, U.K.1
  • 21
    • 0020687017 scopus 로고
    • 10.1016/S0076-6879(83)91020-0 1:CAS:528:DyaL3sXktValt7g%3D
    • CM Wilson 1983 Enzymology 91 236 247 10.1016/S0076-6879(83)91020-0 1:CAS:528:DyaL3sXktValt7g%3D
    • (1983) Enzymology , vol.91 , pp. 236-247
    • Wilson, C.M.1
  • 22
    • 0017184389 scopus 로고
    • 10.1016/0003-2697(76)90527-3 1:CAS:528:DyaE28XksVehtrY%3D
    • MM Bradford 1976 Analytical Biochemistry 72 248 254 10.1016/0003-2697(76) 90527-3 1:CAS:528:DyaE28XksVehtrY%3D
    • (1976) Analytical Biochemistry , vol.72 , pp. 248-254
    • Bradford, M.M.1
  • 24
  • 29
    • 0030596471 scopus 로고    scopus 로고
    • The glycans of horseradish peroxidase
    • DOI 10.1016/0008-6215(96)00073-0
    • BY Yang JSS Gray R Montgomery 1996 Carbohydrate Research 287 203 212 10.1016/0008-6215(96)00073-0 1:CAS:528:DyaK28Xks1CrtLo%3D (Pubitemid 26266989)
    • (1996) Carbohydrate Research , vol.287 , Issue.2 , pp. 203-212
    • Yang, B.Y.1    Gray, J.S.S.2    Montgomery, R.3
  • 30
    • 0742324902 scopus 로고    scopus 로고
    • Horseradish peroxidase: A modern view of a classic enzyme
    • DOI 10.1016/j.phytochem.2003.10.022
    • NC Veitch 2004 Phytochemistry 65 249 259 10.1016/j.phytochem.2003.10.022 1:CAS:528:DC%2BD2cXmvFGntA%3D%3D (Pubitemid 38147341)
    • (2004) Phytochemistry , vol.65 , Issue.3 , pp. 249-259
    • Veitch, N.C.1
  • 31
    • 0021967662 scopus 로고
    • Function of glycoprotein glycans
    • DOI 10.1016/0968-0004(85)90238-5
    • K Olden BA Bernard MJ Humphries TK Yeo KT Yeo SL White, et al. 1985 Trends in Biochemical Sciences 12 78 82 10.1016/0968-0004(85)90238-5 (Pubitemid 15166746)
    • (1985) Trends in Biochemical Sciences , vol.10 , Issue.2 , pp. 78-82
    • Olden, K.1    Bernard, B.A.2    Humphries, M.J.3
  • 32
    • 0033136115 scopus 로고    scopus 로고
    • Functional expression of horseradish peroxidase in E. coli by directed evolution
    • DOI 10.1021/bp990037r
    • Z Lin T Thorsen FH Arnold 1999 Biotechnology Progress 15 467 471 10.1021/bp990037r 1:CAS:528:DyaK1MXit1Squ7w%3D (Pubitemid 29270691)
    • (1999) Biotechnology Progress , vol.15 , Issue.3 , pp. 467-471
    • Lin, Z.1    Thorsen, T.2    Arnold, F.H.3
  • 33
    • 34249777526 scopus 로고    scopus 로고
    • Eris: An automated estimator of protein stability [2]
    • DOI 10.1038/nmeth0607-466, PII NMETH0607-466
    • sh Yin F Ding NV Dokholyan 2007 Nature Methods 4 6 466 467 10.1038/nmeth0607-466 1:CAS:528:DC%2BD2sXlvVykurg%3D (Pubitemid 46852064)
    • (2007) Nature Methods , vol.4 , Issue.6 , pp. 466-467
    • Yin, S.1    Ding, F.2    Dokholyan, N.V.3
  • 34
    • 0033543221 scopus 로고    scopus 로고
    • Luminol activity of horseradish peroxidase mutants mimicking a proposed binding site for luminol in Arthromyces ramosus peroxidase
    • DOI 10.1021/bi9907328
    • M Tanaka K Ishimori I Morishima 1999 Biochemistry 38 10463 10473 10.1021/bi9907328 1:CAS:528:DyaK1MXksFGisbc%3D (Pubitemid 29383418)
    • (1999) Biochemistry , vol.38 , Issue.32 , pp. 10463-10473
    • Tanaka, M.1    Ishimori, K.2    Morishima, I.3
  • 35
    • 47749142434 scopus 로고    scopus 로고
    • 10.1016/j.biochi.2008.04.009 1:CAS:528:DC%2BD1cXptVajtb8%3D
    • BJ Ryan MJ O'Connell C O'Fagain 2008 Biochimie 90 9 1389 1396 10.1016/j.biochi.2008.04.009 1:CAS:528:DC%2BD1cXptVajtb8%3D
    • (2008) Biochimie , vol.90 , Issue.9 , pp. 1389-1396
    • Ryan, B.J.1    O'Connell, M.J.2    O'Fagain, C.3
  • 44
    • 0022428140 scopus 로고
    • 10.1021/bi00347a027 1:CAS:528:DyaL28XhslSnug%3D%3D
    • TW Thannhauser HA Scheraga 1985 Biochemistry 24 7681 7688 10.1021/bi00347a027 1:CAS:528:DyaL28XhslSnug%3D%3D
    • (1985) Biochemistry , vol.24 , pp. 7681-7688
    • Thannhauser, T.W.1    Scheraga, H.A.2
  • 46
    • 0026065533 scopus 로고
    • 10.1093/protein/4.3.283 1:CAS:528:DyaK3MXisVejtr0%3D
    • HT Wright 1991 Protein Engineering 4 283 294 10.1093/protein/4.3.283 1:CAS:528:DyaK3MXisVejtr0%3D
    • (1991) Protein Engineering , vol.4 , pp. 283-294
    • Wright, H.T.1


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