Genetically engineered horseradish peroxidase for facilitated purification from baculovirus cultures by cation-exchange chromatography

Journal of Biotechnology

Volumn 118, Issue 4, 2005, Pages 363-369

  Levin, Gustavo ^a   Mendive, Fernando ^a   Targovnik, Héctor M ^a   Cascone, Osvaldo ^a   Miranda, María V ^a  

^a UNIVERSIDAD DE BUENOS AIRES   (Argentina)

Author keywords

Fusion tail; Immobilised metal ion affinity chromatography; Ion exchange chromatography; Peroxidase; Purification

Indexed keywords

CELLS; CHROMATOGRAPHIC ANALYSIS; DNA; ENZYMES; PEROXIDES; POSITIVE IONS; PURIFICATION;

AUTOGRAPHA CALIFORNICA; CATION EXCHANGE CHROMATOGRAPHY; HORSERADISH PEROXIDASE; SPODOPTERA FRUGIPERDA;

GENETIC ENGINEERING;

ARGININE; COMPLEMENTARY DNA; HISTIDINE; HORSERADISH PEROXIDASE; ISOENZYME; RECOMBINANT ENZYME;

ARTICLE; AUTOGRAPHA CALIFORNICA; BACULOVIRUS; BACULOVIRUS EXPRESSION SYSTEM; COMPARATIVE STUDY; CONTROLLED STUDY; CULTURE MEDIUM; GENE EXPRESSION; GENETIC ENGINEERING; IMMOBILIZED METAL AFFINITY CHROMATOGRAPHY; INSECT CELL; ION EXCHANGE CHROMATOGRAPHY; ISOELECTRIC FOCUSING; ISOELECTRIC POINT; LEPIDOPTERA; NONHUMAN; POLYHEDROSIS VIRUS; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PURIFICATION; VIRUS CULTURE;

BACULOVIRIDAE; CHROMATOGRAPHY, ION EXCHANGE; GENE EXPRESSION; HORSERADISH PEROXIDASE; PEPTIDES; PROTEIN ENGINEERING; RECOMBINANT FUSION PROTEINS;

ARMORACIA RUSTICANA; AUTOGRAPHA CALIFORNICA NUCLEOPOLYHEDROVIRUS; INSECTA; SPODOPTERA FRUGIPERDA;

EID: 23044476987     PISSN: 01681656     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2005.05.015     Document Type: Article

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