An optimized mRFP-based bimolecular fluorescence complementation system for the detection of protein-protein interactions in planta

Journal of Virological Methods

Volumn 174, Issue 1-2, 2011, Pages 158-165

  Zilian, Eva ^a   Maiss, Edgar ^a  

^a LEIBNIZ UNIVERSITY HANNOVER   (Germany)

Author keywords

Bimolecular fluorescence complementation (BiFC); Capsicum chlorosis virus; Plum pox virus; Protein protein interaction; Red fluorescent protein

Indexed keywords

COAT PROTEIN; MONOMERIC RED FLUORESCENT PROTEIN; PROTEIN BC1; PROTEIN BV1; RED FLUORESCENT PROTEIN; UNCLASSIFIED DRUG; VIRUS PROTEIN;

AMINO ACID SEQUENCE; AMINO TERMINAL SEQUENCE; ARTICLE; BIMOLECULAR FLUORESCENCE COMPLEMENTATION SYSTEM; CAPSICUM CHLOROSIS; CARBOXY TERMINAL SEQUENCE; CONFOCAL LASER MICROSCOPY; CONTROLLED STUDY; FLUORESCENCE ANALYSIS; GENE FUSION; MOSAIC VIRUS; NICOTIANA BENTHAMIANA; NONHUMAN; PEPPER; PLANT; PLANT CELL; PLANT PATHOGEN INTERACTION; PLANT VIRUS; PLASMID; PRIORITY JOURNAL; PROCESS OPTIMIZATION; PROTEIN ANALYSIS; PROTEIN PROTEIN INTERACTION; TOBACCO MOSAIC VIRUS; TOMATO YELLOW LEAF CURL THAILAND VIRUS; TWO HYBRID SYSTEM; YEAST;

BEGOMOVIRUS; CAPSID PROTEINS; GENES, REPORTER; GENETIC VECTORS; LUMINESCENT PROTEINS; MICROSCOPY, CONFOCAL; PLASMIDS; PROTEIN BINDING; PROTEIN INTERACTION MAPPING; STAINING AND LABELING; TOBACCO; TOBACCO MOSAIC VIRUS; TOSPOVIRUS;

CAPSICUM CHLOROSIS VIRUS; NICOTIANA BENTHAMIANA; PLUM POX VIRUS; TOBACCO MOSAIC VIRUS; TOMATO YELLOW LEAF CURL THAILAND VIRUS;

EID: 79955769528     PISSN: 01660934     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jviromet.2011.03.032     Document Type: Article

References (44)

1. Atmakuri K., Ding Z., Christie P.J. VirE2, a type IV secretion substrate, interacts with the VirD4 transfer protein at cell poles of Agrobacterium tumefaciens. Mol. Microbiol. 2003, 49:1699-1713.
2. Bendahmane M., Koo M., Karrer E., Beachy R.N. Display of epitopes on the surface of Tobacco mosaic virus: impact of charge and isoelectric point of the epitope on virus-host interactions. J. Mol. Biol. 1999, 290:9-20.
3. Berger P.H., Hunt A.G., Domier L.L., Hellmann G.M., Stram Y., Thornbury D.W., Pirone T.P. Expression in transgenic plants of a viral gene product that mediates insect transmission of potyviruses. Proc. Natl. Acad. Sci. U.S.A. 1989, 86:8402-8406.
4. Bevan M. Binary Agrobacterium vectors for plant transformation. Nucleic Acids Res. 1984, 12:8711-8721.
5. Bhat R.A., Lahaye T., Panstruga R. The visible touch: in planta visualization of protein-protein interactions by fluorophore-based methods. Plant Methods 2006, 2:12.
6. Blawid R., Van D.T., Maiss E. Transreplication of a Tomato yellow leaf curl Thailand virus DNA-B and replication of a DNA β component by Tomato leaf curl Vietnam virus and Tomato yellow leaf curl Vietnam virus. Virus Res. 2008, 136:107-117.
7. Blondel M., Bach S., Bamps S., Dobbelaere J., Wiget P., Longaretti C., Barral Y., Meijer L., Peter M. Degradation of Hof1 by SCF(Grr1) is important for actomyosin contraction during cytokinesis in yeast. EMBO J. 2005, 24:1440-1452.
8. Bracey L.T., Paigen K. Changes in translational yield regulate tissue-specific expression of beta-glucuronidase. Proc. Natl. Acad. Sci. U.S.A. 1987, 84:9020-9024.
9. Bracha-Drori K., Shichrur K., Katz A., Oliva M., Angelovici R., Yalovsky S., Ohad N. Detection of protein-protein interactions in plants using bimolecular fluorescence complementation. Plant J. 2004, 40:419-427.
10. Deblaere R., Bytebier B., De Greve H., Deboeck F., Schell J., Van Montagu M., Leemans J. Efficient octopine Ti plasmid-derived vectors for Agrobacterium-mediated gene transfer to plants. Nucleic Acids Res. 1985, 13:4777-4788.
11. Fan J.Y., Cui Z.-Q., Wei H.-P., Zhang Z.-P., Zhou Y.-F., Wang Y.-P., Zhang X.-E. Split mCherry as a new red bimolecular fluorescence complementation system for visualizing protein-protein interactions in living cells. Biochem. Biophys. Res. Commun. 2008, 367:47-53.
12. Fields S., Song O. A novel genetic system to detect protein-protein interactions. Nature 1989, 340:245-246.
13. Frischmuth S., Kleinow T., Aberle H.-J., Wege C., Hülser D., Jeske H. Yeast two-hybrid systems confirm the membrane-association and oligomerization of BC1 but do not detect an interaction of the movement proteins BC1 and BV1 of Abutilon mosaic geminivirus. Arch. Virol. 2004, 149:2349-2364.
14. Gordon G.W., Berry G., Liang X.H., Levine B., Herman B. Quantitative fluorescence resonance energy transfer measurements using fluorescence microscopy. Biophys. J. 1998, 74:2702-2713.
15. Guo D., Rajamäki M.L., Saarma M., Valkonen J.P. Towards a protein interaction map of potyviruses: protein interaction matrixes of two potyviruses based on the yeast two-hybrid system. J. Gen. Virol. 2001, 82:935-939.
16. Hajdukiewicz P., Svab Z., Maliga P. The small, versatile pPZP family of Agrobacterium binary vectors for plant transformation. Plant Mol. Biol. 1994, 25:989-994.
17. Hall R.A. Studying protein-protein interactions via blot overlay or far western blot. Methods Mol. Biol. 2004, 261:167-174.
18. Hoff B., Kück U. Use of bimolecular fluorescence complementation to demonstrate transcription factor interaction in nuclei of living cells from the filamentous fungus Acremonium chrysogenum. Curr. Genet. 2005, 47:132-138.
19. Hu C.-D., Chinenov Y., Kerppola T.K. Visualization of interactions among bZIP and Rel family proteins in living cells using bimolecular fluorescence complementation. Mol. Cell 2002, 9:789-798.
20. Hu C.-D., Kerppola T.K. Simultaneous visualization of multiple protein interactions in living cells using multicolor fluorescence complementation analysis. Nat. Biotechnol. 2003, 21:539-545.
21. Jach G., Pesch M., Richter K., Frings S., Uhrig J.F. An improved mRFP1 adds red to bimolecular fluorescence complementation. Nat. Methods 2006, 3:597-600.
22. Kang S.-H., Lim W.-S., Kim K.-H. A protein interaction map of Soybean mosaic virus strain G7H based on the yeast two-hybrid system. Mol. Cells 2004, 18:122-126.
23. Kang S.-H., Lim W.-S., Hwang S.-H., Park J.-W., Choi H.-S., Kim K.-H. Importance of the C-terminal domain of Soybean mosaic virus coat protein for subunit interactions. J. Gen. Virol. 2006, 87:225-229.
24. Kerppola T.K. Design and implementation of bimolecular fluorescence complementation (BiFC) assays for the visualization of protein interactions in living cells. Nat. Protoc. 2006, 1:1278-1286.
25. Kim M.H., Roh H.E., Lee M.N., Hur M.W. New fast BiFC plasmid assay system for in vivo protein-protein interactions. Cell. Physiol. Biochem. 2007, 20:703-714.
26. Knierim D., Blawid R., Maiss E. The complete nucleotide sequence of a Capsicum chlorosis virus isolate from Lycopersicum esculentum in Thailand. Arch. Virol. 2006, 151:1761-1782.
27. Lin L., Shi Y., Luo Z., Lu Y., Zheng H., Yan F., Chen J., Chen J., Adams M.J., Wu Y. Protein-protein interactions in two potyviruses using the yeast two-hybrid system. Virus Res. 2009, 142:36-40.
28. Maiss E., Timpe U., Brisske-Rode A., Lesemann D.-E., Casper R. Infectious in vivo transcripts of a Plum pox potyvirus full-length cDNA clone containing the Cauliflower mosaic virus 35S RNA promoter. J. Gen. Virol. 1992, 73:709-713.
29. Masters S.C. Co-immunoprecipitation from transfected cells. Methods Mol. Biol. 2004, 261:337-348.
30. Namba K., Pattanayek R., Stubbs G. Visualization of protein-nucleic acid interactions in a virus refined structure of intact Tobacco mosaic virus at 2.9Å resolution by X-ray fiber diffraction. J. Mol. Biol. 1989, 208:307-325.
31. Phizicky E.M., Fields S. Protein-protein interactions: methods for detection and analysis. Microbiol. Rev. 1995, 59:94-123.
32. Schägger H., von Jagow G. Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100kDa. Anal. Biochem. 1987, 166:368-379.
33. Schwartz T.U., Walczak R., Blobel G. Circular permutation as a tool to reduce surface entropy triggers crystallization of the signal recognition particle receptor beta subunit. Protein Sci. 2004, 13:2814-2818.
34. Sekar R.B., Periasamy A. Fluorescence resonance energy transfer (FRET) microscopy imaging of live cell protein localizations. J. Cell Biol. 2003, 160:629-633.
35. Shen W.T., Wang M.Q., Yan P., Gao L., Zhou P. Protein interaction matrix of Papaya ringspot virus type P based on a yeast two-hybrid system. Acta Virol. 2010, 54:49-54.
36. Snippe M., Borst J.W., Goldbach R., Kormelink R. The use of fluorescence microscopy to visualise homotypic interactions of tomato spotted wilt virus nucleocapsid protein in living cells. J. Virol. Methods 2005, 125:15-22.
37. Stolpe T., Süsslin C., Marrocco K., Nick P., Kretsch T., Kircher S. In planta analysis of protein-protein interactions related to light signaling by bimolecular fluorescence complementation. Protoplasma 2005, 226:137-146.
38. Sung M.-K., Huh W.-K. Bimolecular fluorescence complementation analysis system for in vivo detection of protein-protein interaction in Saccharomyces cerevisiae. Yeast 2007, 24:767-775.
39. Toepfer R., Matzeit V., Gronenborn B., Schell J., Steinbiss H.-H. A set of plant expression vectors for transcriptional and translational fusions. Nucleic Acids Res. 1987, 15:5890.
40. Towbin H., Staehelin T., Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc. Natl. Acad. Sci. U.S.A. 1979, 76:4350-4354.
41. Uhrig J.F., Soellick T.-R., Minke C.J., Philipp Q., Kellmann J.-W., Schreier P.H. Homotypic interaction and multimerization of nucleocapsid protein of Tomato spotted wilt tospovirus: identification and characterization of two interacting domains. Proc. Natl. Acad. Sci. U.S.A. 1999, 96:55-60.
42. Voinnet O., Rivas S., Mestre P., Baulcombe D. An enhanced transient expression system in plants based on suppression of gene silencing by the p19 protein of Tomato bushy stunt virus. Plant J. 2003, 33:949-956.
43. Walter M., Chaban C., Schütze K., Batistic O., Weckermann K., Näke C., Blazevic D., Grefen C., Schumacher K., Oecking C., Harter K., Kudla J. Visualization of protein interactions in living plant cells using bimolecular fluorescence complementation. Plant J. 2004, 40:428-438.
44. Xiang C., Han P., Lutziger I., Wang K., Oliver D.J. A mini binary vector series for plant transformation. Plant Mol. Biol. 1999, 40:711-717.