Enhanced PKCε mediated phosphorylation of connexin43 at serine 368 by a carboxyl-terminal mimetic peptide is dependent on injury

Channels

Volumn 5, Issue 3, 2011, Pages

  Palatinus, Joseph A ^a   Rhett, J Matthew ^a   Gourdie, Robert G ^a  

^a MEDICAL UNIVERSITY OF SOUTH CAROLINA   (United States)

Author keywords

Connexon; Cx43; Gap junction; Hemichannel; PKC epsilon

Indexed keywords

ALPHA CARBOXY TERMINAL PEPTIDE 1; CONNEXIN 43; PEPTIDE DERIVATIVE; PROTEIN KINASE C; SERINE; UNCLASSIFIED DRUG; BIOMIMETIC MATERIAL; GJA1 PROTEIN, HUMAN; PEPTIDE; PROTEIN KINASE C EPSILON;

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL MODEL; ARTICLE; CARBOXY TERMINAL SEQUENCE; CELL CULTURE; CELLULAR STRESS RESPONSE; CONTROLLED STUDY; DENSITOMETRY; FEMALE; GAP JUNCTION; HEART INJURY; HEART MUSCLE; HEART MUSCLE INJURY; HELA CELL; HUMAN; HUMAN CELL; IN VITRO STUDY; INHIBITION KINETICS; MONOLAYER CULTURE; MOUSE; NONHUMAN; PROTEIN ANALYSIS; PROTEIN EXPRESSION; PROTEIN PHOSPHORYLATION; PROTEIN PROTEIN INTERACTION; WESTERN BLOTTING; WOUND; ANIMAL; DRUG EFFECT; GENETICS; HEART MUSCLE ISCHEMIA; METABOLISM; PHOSPHORYLATION;

MAMMALIA;

ANIMALS; BIOMIMETIC MATERIALS; CONNEXIN 43; FEMALE; HELA CELLS; HUMANS; MICE; MYOCARDIAL ISCHEMIA; MYOCARDIUM; PEPTIDES; PHOSPHORYLATION; PROTEIN KINASE C-EPSILON;

EID: 79955745771     PISSN: 19336950     EISSN: 19336969     Source Type: Journal    
DOI: 10.4161/chan.5.3.15834     Document Type: Article

References (26)

1. Beyer EC, Paul DL, Goodenough DA. Connexin43: a protein from rat heart homologous to a goap junction protein from liver. J Cell Biol 1987; 105:2621-9. (Pubitemid 18017692)
2. Gourdie RG, Green CR, Severs NJ. Gap junction distribution in adult mammalian myocardium revealed by an anti-peptide antibody and laser scanning confocal microscopy. J Cell Sci 1991; 99:41-55.
3. Jansen JA, van Veen TA, de Bakker JM, van Rijen HV. Cardiac connexins and impulse propagation. J Mol Cell Cardiol 2010; 48:76-82.
4. Delmar M, Coombs W, Sorgen P, Duffy HS, Taffet SM. Structural bases for the chemical regulation of Connexin43 channels. Cardiovasc Res 2004; 62:268-75. (Pubitemid 38496540)
5. Severs NJ, Dupont E, Thomas N, Kaba R, Rothery S, Jain R, et al. Alterations in cardiac connexin expression in cardiomyopathies. Adv Cardiol 2006; 42:228-42. (Pubitemid 43805867)
6. Palatinus JA, Rhett JM, Gourdie RG. Translational lessons from scarless healing of cutaneous wounds and regenerative repair of the myocardium. J Mol Cell Cardiol 2010; 48:550-7.
7. Gutstein DE, Morley GE, Tamaddon H, Vaidya D, Schneider MD, Chen J, et al. Conduction slowing and sudden arrhythmic death in mice with cardiac-restricted inactivation of connexin43. Circ Res 2001; 88:333-9. (Pubitemid 32173758)
8. Saffitz JE, Schuessler RB, Yamada KA. Mechanisms of remodeling of gap junction distributions and the development of anatomic substrates of arrhythmias. Cardiovasc Res 1999; 42:309-17. (Pubitemid 29243159)
9. Spach MS, Heidlage JF, Dolber PC, Barr RC. Electrophysiological effects of remodeling cardiac gap junctions and cell size: experimental and model studies of normal cardiac growth. Circ Res 2000; 86:302-11. (Pubitemid 30108824)
10. Poelzing S, Rosenbaum DS. Altered connexin43 expression produces arrhythmia substrate in heart failure. Am J Physiol Heart Circ Physiol 2004; 287:1762-70.
11. Wit AL, Duffy HS. Drug development for treatment of cardiac arrhythmias: targeting the gap junctions. Am J Physiol Heart Circ Physiol 2008; 294:16-8.
12. Smith JH, Green CR, Peters NS, Rothery S, Severs NJ. Altered patterns of gap junction distribution in ischemic heart disease. An immunohistochemical study of human myocardium using laser scanning confocal microscopy. Am J Pathol 1991; 139:801-21.
13. Peters NS, Green CR, Poole-Wilson PA, Severs NJ. Reduced content of connexin43 gap junctions in ventricular myocardium from hypertrophied and ischemic human hearts. Circulation 1993; 88:864-75. (Pubitemid 23264001)
14. Cabo C, Yao J, Boyden PA, Chen S, Hussain W, Duffy HS, et al. Heterogeneous gap junction remodeling in reentrant circuits in the epicardial border zone of the healing canine infarct. Cardiovasc Res 2006; 72:241-9. (Pubitemid 44511911)
15. Yao J, Hussain W, Patel P, Peters NS, Boyden PA, Wit AL. remodeling of gap junctional channel function in epicardial border zone of healing canine infarct. Circ Res 2003; 92:437-43. (Pubitemid 36298204)
16. O'Quinn MP, Palatinus JA, Harris BS, Hewett KW, Gourdie RG. A Peptide Mimetic of the Connexin43 Carboxyl Terminus Reduces Gap Junction Remodeling and Induced Arrhythmia Following Ventricular Injury. Circ Res 2011.
17. Hunter AW, Barker RJ, Zhu C, Gourdie RG. Zonula occludens-1 alters connexin43 gap junction size and organization by influencing channel accretion. Mol Biol Cell 2005; 16:5686-98. (Pubitemid 41752218)
18. Rhett JM, Jourdan J, Gourdie RG. Connexin43 Connexon to Gap Junction Transition Is Regulated by Zonula Occludens-1. Mol Biol Cell 2011.
19. Bruce AF, Rothery S, Dupont E, Severs NJ. Gap junction remodelling in human heart failure is associated with increased interaction of connexin43 with ZO-1. Cardiovasc Res 2008; 77:757-65. (Pubitemid 351301863)
20. Palatinus JA, O'Quinn MP, Barker RJ, Harris BS, Jourdan J, Gourdie RG. ZO-1 eetermines adherens and gap junction localization at intercalated disks. Am J Physiol Heart Circ Physiol 2010.
21. Ghatnekar GS, O'Quinn MP, Jourdan LJ, Gurjarpadhye AA, Draughn RL, Gourdie RG. Connexin43 carboxyl-terminal peptides reduce scar progenitor and promote regenerative healing following skin wounding. Regen Med 2009; 4:205-23.
22. Rhett JM, Ghatnekar GS, Palatinus JA, O'Quinn M, Yost MJ, Gourdie RG. Novel therapies for scar reduction and regenerative healing of skin wounds. Trends in biotechnology 2008; 26:173-80.
23. Ek-Vitorin JF, King TJ, Heyman NS, Lampe PD, Burt JM. Selectivity of connexin 43 channels is regulated through protein kinase C-dependent phosphorylation. Circ Res 2006; 98:1498-505. (Pubitemid 44297072)
24. Peters NS, Coromilas J, Severs NJ, Wit AL. Disturbed connexin43 gap junction distribution correlates with the location of reentrant circuits in the epicardial border zone of healing canine infarcts that cause ventricular tachycardia. Circulation 1997; 95:988-96. (Pubitemid 27091545)
25. Doble BW, Dang X, Ping P, Fandrich RR, Nickel BE, Jin Y, et al. Phosphorylation of serine 262 in the gap junction protein connexin-43 regulates DNA synthesis in cell-cell contact forming cardiomyocytes. J Cell Sci 2004; 117:507-14. (Pubitemid 38181211)
26. Ping P, Zhang J, Qiu Y, Tang XL, Manchikalapudi S, Cao X, et al. Ischemic preconditioning induces selective translocation of protein kinase C isoforms epsilon and eta in the heart of conscious rabbits without subcellular redistribution of total protein kinase C activity. Circ Res 1997; 81:404-14. (Pubitemid 27362528)