A new method for sex determination based on detection of SRY, STS and amelogenin gene regions with simultaneous amplification of their homologous sequences by a multiplex PCR

Acta Medica Okayama

Volumn 65, Issue 2, 2011, Pages 113-122

  Morikawaa, Toshio ^a,b   Yamamoto, Yuji ^a   Miyaish, Satoru ^a  

^a OKAYAMA UNIVERSITY   (Japan)

^b Forensic Science Laboratory   (Japan)

Author keywords

Forensic casework; Multiplex PCR; Sex determination; SRY (sex determining region Y)

Indexed keywords

AMELOGENIN; SRY PROTEIN, HUMAN; STERYL SULFATASE; TESTIS DETERMINING FACTOR;

ARTICLE; FEMALE; FORENSIC MEDICINE; GENETICS; HUMAN; MALE; METHODOLOGY; POLYMERASE CHAIN REACTION; SEX DETERMINATION; SPECIES DIFFERENCE;

AMELOGENIN; FEMALE; FORENSIC MEDICINE; HUMANS; MALE; POLYMERASE CHAIN REACTION; SEX DETERMINATION ANALYSIS; SEX-DETERMINING REGION Y PROTEIN; SPECIES SPECIFICITY; STERYL-SULFATASE;

EID: 79955743825     PISSN: 0386300X     EISSN: 0386300X     Source Type: Journal    
DOI: None     Document Type: Article

References (22)

1. von Wurmb-Schwark N, Bosinski H and Ritz-Timme S: What do the X and Y chromosomes tell us about sex and gender in forensic case analysis? J Forensic Leg Med (2007) 14: 27-30
2. Pfitzinger H, Ludes B and Mangin P: Sex determination of forensic samples: co-amplification and simultaneous detection of a Y-specific and an X-specific DNA sequence. Int J Legal Med (1993) 105: 213-216
3. Yamamoto Y, Nakanishi A, Semba S and Ishizu H: Sex determination of bloodstains by polymerase chain reaction (PCR) of Y-choromosome-specific alphoid repeat sequences. Acta Crim Japon(1991)57: 187-191
4. Semba S, Yamamoto Y and Ishizu H: Sex determination from blood and bloodstains by polymerase chain reaction (PCR). Jpn J Legal Med (1994) 48: 7-18
5. Murakami H, Yamamoto Y, Yoshitome K, Ono T, Okamoto O, Shigeta Y, Doi Y, Miyaishi S and Ishizu H: Forensic study of sex determination using PCR on teeth samples. Acta Med Okayama (2000)54: 21-32
6. Santos FR, Pandya A and Tyler-Smith C: Reliability of DNA-based sex tests. Nat Genet (1998) 18: 103
7. Steinlechner M, Berger B, Niederstatter H and Parson W: Rare failures in the amelogenin sex test. Int J Legal Med (2002) 116: 117-123
8. Thangaraj K, Reddy AG and Singh L: Is the amelogenin gene reliable for gender identification in forensic casework and prenatal diagnosis? Int J Legal Med (2002) 116: 121-123
9. Drobnič K: A new primer set in a SRY gene for sex identification. Int Congr Ser (2006) 1288: 268-27
10. Nakahori Y, Hamano K, Iwaya M and Nakagome Y: Sex identification by polymerase chain reaction using X-Y homologous primer. Am J Hum Genet (1991) 39: 472-473
11. Sullivan KM, Mannucci A, Kimpton CP and Gill P: A rapid and quantitative DNA sex test: fluorescence-based PCR analysis of X.Y homologous gene amelogenin. Biotechniques (1993) 15: 636-641
12. Roffey PE, Eckhoff Cl and Kuhl JL: A rare mutation in the amelogenin gene and its potential investigative ramifications. J Forensic Sci (2000)45: 1016-1019
13. Michael A and Brauner P: Erroneous gender identification by the amelogenin sex test. J Forensic Sci (2004) 49: 258-259
14. Lattanzi W, Di Giacomo MC, Lenato GM, Chimienti G, Voglino G, Resta N, Pepe G and Guanti G: A large interstitial deletion encompassing the amelogenin gene on the short arm of the Y chromosome. Hum Genet (2005) 116: 395-401
15. Kashyap VK, Sahoo S, Sitalaximi T and Trivedi R: Deletions in the Y-derived amelogenin gene fragment in the Indian population. BMC Med Genet (2006) 7: 37
16. P11.2 (DYS458-MSY1-AMEL-Y) deletion. Forensic Sci Int (2007) 166: 115-120
17. Cadenas AM, Regueiro M, Gayden T, Singh N, Zhivotovsky LA, Underhill PA and Herrera RJ: Male amelogenin dropouts: phyloge netic context, origins and implications. Forensic Sci Int (2007) 166: 155-163
18. Yong RY, Gan LS, Chang YM and Yap EP: Molecular characterization of a polymorphic 3-Mb deletion at chromosome Yp11.2 containing the AMELY locus in Singapore and Malaysia populations. Hum Genet (2007) 122: 237-249
19. Jobling MA, Lo IC, Turner DJ, Bowden GR, Lee AC, Xue Y, Carvalho-Silva D, Hurles ME, Adams SM, Chang YM, Kraaijenbrink T, Henke J, Guanti G, McKeown B, van Oorschot RA, Mitchell RJ, de Knijff P, Tyler-Smith C and Parkin EJ: Structural variation on the short arm of the human Y chromosome: recurrent multigene deletions encompassing Amelogenin Y. Hum Mol Genet (2007) 16: 307-316
20. Kumagai R, Sasaki Y, Tokuta T, Biwasaka H and Aoki Y: DNA analysis of family members with deletion in Yp11.2 region containing amelogenin locus. Leg Med (Tokyo) (2008) 10: 39-42
21. Brownstein MJ, Carpten JD and Smith JR: Modulation of non-templated nucleotide addition by Taq DNA polymerase: primer modifications that facilitate genotyping. Biotechniques (1996) 20: 1004-1010
22. Doi Y, Yamamoto Y, Inagaki S, Shigeta Y, Miyaishi S and Ishizu H: A new method for ABO genotyping using a multiplex single-base primer extension reaction and its application to forensic casework samples. Legal Med (2004) 6: 213-223