Modulation of non-templated nucleotide addition by Taq DNA polymerase: Primer modifications that facilitate genotyping

BioTechniques

Volumn 20, Issue 6, 1996, Pages 1004-1010

  Brownstein, Michael J ^c   Carpten, John D ^a   Smith, Jeffrey R ^b  

^a NATIONAL HUMAN GENOME RESEARCH INSTITUTE   (United States)

^b UNIVERSITY OF MICHIGAN   (United States)

^c NATIONAL INSTITUTE OF MENTAL HEALTH   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BIOCHEMICAL MARKER; DNA POLYMERASE;

ADENYLATION; AMINO ACID SEQUENCE; ARTICLE; DNA MODIFICATION; DNA TEMPLATE; GENOTYPE; MOLECULAR CLONING; NUCLEOTIDE SEQUENCE;

BASE SEQUENCE; CLONING, MOLECULAR; DNA PRIMERS; DNA-DIRECTED DNA POLYMERASE; GENOTYPE; OLIGONUCLEOTIDES; POLYMERASE CHAIN REACTION; RNA-DIRECTED DNA POLYMERASE; TAQ POLYMERASE;

EID: 0001506104     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/96206st01     Document Type: Article

References (8)

1. Clark, J.M. 1988. Novel non-templated nucleotide addition reactions catalyzed by prokaryotic and eucaryolic DNA polymerases. Nucleic Acids Res. 16:9677-9686.
2. Holton, T.A. and M.W. Graham. 1991. A simple and efficient method for direct cloning of PCR products using ddT-tailed vectors. Nucleic Acids Res. 19:1156.
3. Hu, G. 1993. DNA polymerase-catalyzed addition of nontemplated extra nucleotides to the 3′ end of a DNA fragment. DNA Cell Biol. 12:763-770.
4. Marchuk, D., M. Drumm, A. Saulino and F.S. Collins. 1991. Construction of T-vectors, a rapid and general system for direct cloning of unmodified PCR products. Nucleic Acids Res. 19:1154.
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7. Smith, J.R., J.D. Carpten, M. Brownstein, S. Ghosh, V. Magnuson, D.A. Gilbert, J.M. Trent and F.S. Collins. 1995. An approach to genotyping errors caused by non-templated nucleotide addition by Taq DNA polymerase. Genome Res. 5:312-317.
8. Ziegle, J.S., Y. Su, K.P. Corcoran, L. Nie, P.E. Mayrand, L.B. Hoff, L.J. McBride, M.N. Kronick and S.R. Diehl. 1992. Application of automated DNA sizing technology for genotyping microsatellite loci. Genomics 14: 1026-1031.