Rad is a p53 direct transcriptional target that inhibits cell migration and is frequently silenced in lung carcinoma cells

Journal of Molecular Medicine

Volumn 89, Issue 5, 2011, Pages 481-492

  Hsiao, Bo Yuan ^a   Chen, Chun Chin ^a   Hsieh, Pei Chen ^a   Chang, Tsun Kai ^a   Yeh, Yi Chen ^a,b   Wu, Yu Chung ^b   Hsu, Han Shui ^a   Wang, Fung Fang ^a   Chou, Teh Ying ^a,b  

^a NATIONAL YANG MING UNIVERSITY   (Taiwan)

^b TAIPEI VETERANS GENERAL HOSPITAL   (Taiwan)

Author keywords

Cell migration; Lung carcinoma; p53; Rad GTPase; Transcriptional regulation

Indexed keywords

CAMPTOTHECIN; COFILIN; DOXORUBICIN; MESSENGER RNA; MITOMYCIN C; PROTEIN P53; PROTEIN RAD; RHO KINASE INHIBITOR; UNCLASSIFIED DRUG;

AGED; ARTICLE; BINDING SITE; CARCINOMA CELL; CELL INVASION; CELL MIGRATION; CLINICAL ARTICLE; DNA DAMAGE; FEMALE; GENE ACTIVATION; GENE OVEREXPRESSION; GENE SILENCING; GENETIC TRANSCRIPTION; HISTONE ACETYLATION; HUMAN; HUMAN CELL; HUMAN TISSUE; LUNG CARCINOMA; LUNG NON SMALL CELL CANCER; MALE; NUCLEOTIDE SEQUENCE; PROMOTER REGION; PROTEIN EXPRESSION; PROTEIN PHOSPHORYLATION; PROTEIN PROTEIN INTERACTION; SIGNAL TRANSDUCTION; UPREGULATION;

ACTIN DEPOLYMERIZING FACTORS; BLOTTING, WESTERN; CARCINOMA; CELL LINE; CELL LINE, TUMOR; CELL MOVEMENT; CHROMATIN IMMUNOPRECIPITATION; ELECTROPHORETIC MOBILITY SHIFT ASSAY; FLUORESCENT ANTIBODY TECHNIQUE; HUMANS; IMMUNOHISTOCHEMISTRY; LUNG NEOPLASMS; RAS PROTEINS; TUMOR SUPPRESSOR PROTEIN P53;

EID: 79955710573     PISSN: 09462716     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00109-010-0717-z     Document Type: Article

References (34)

1. D Menendez A Inga MA Resnick 2009 The expanding universe of p53 targets Nat Rev Cancer 9 724 737 19776742 10.1038/nrc2730 1:CAS:528:DC%2BD1MXhtFKmsb7M
2. AJ Levine M Oren 2009 The first 30 years of p53: growing ever more complex Nat Rev Cancer 9 749 758 19776744 10.1038/nrc2723 1:CAS:528: DC%2BD1MXhtFKmsb%2FI
3. KH Vousden C Prives 2009 Blinded by the light: the growing complexity of p53 Cell 137 413 431 19410540 10.1016/j.cell.2009.04.037 1:CAS:528: DC%2BD1MXlvFams74%3D
4. BC Lewis DS Klimstra ND Socci S Xu JA Koutcher HE Varmus 2005 The absence of p53 promotes metastasis in a novel somatic mouse model for hepatocellular carcinoma Mol Cell Biol 25 1228 1237 15684377 10.1128/MCB.25.4.1228-1237.2005 1:CAS:528:DC%2BD2MXhs1KgsLc%3D (Pubitemid 40204903)
5. UK Mukhopadhyay AS Mak 2009 p53: is the guardian of the genome also a suppressor of cell invasion? Cell Cycle 8 2481 19657223 10.4161/cc.8.16.9269 1:CAS:528:DC%2BC3cXjsl2murs%3D
6. T Sumi K Matsumoto Y Takai T Nakamura 1999 Cofilin phosphorylation and actin cytoskeletal dynamics regulated by rho- and Cdc42-activated LIM-kinase 2 J Cell Biol 147 1519 1532 10613909 10.1083/jcb.147.7.1519 1:CAS:528: DC%2BD3cXis12gtw%3D%3D (Pubitemid 30027406)
7. E Sahai CJ Marshall 2003 Differing modes of tumour cell invasion have distinct requirements for Rho/ROCK signalling and extracellular proteolysis Nat Cell Biol 5 711 719 12844144 10.1038/ncb1019 1:CAS:528:DC%2BD3sXlvV2gtrg%3D (Pubitemid 36986778)
8. E Sahai CJ Marshall 2002 RHO-GTPases and cancer Nat Rev Cancer 2 133 142 12635176 10.1038/nrc725
9. A Alexandrova A Ivanov P Chumakov B Kopnin J Vasiliev 2000 Changes in p53 expression in mouse fibroblasts can modify motility and extracellular matrix organization Oncogene 19 5826 5830 11126371 10.1038/sj.onc.1203944 1:CAS:528:DC%2BD3cXoslylsbc%3D
10. G Gadea M de Toledo C Anguille P Roux 2007 Loss of p53 promotes RhoA-ROCK-dependent cell migration and invasion in 3D matrices J Cell Biol 178 23 30 17606864 10.1083/jcb.200701120 1:CAS:528:DC%2BD2sXnsVajurw%3D (Pubitemid 47026399)
11. G Gadea L Lapasset C Gauthier-Rouviere P Roux 2002 Regulation of Cdc42-mediated morphological effects: a novel function for p53 EMBO J 21 2373 2382 12006490 10.1093/emboj/21.10.2373 1:CAS:528:DC%2BD38XktFChu7Y%3D (Pubitemid 34546710)
12. SP Wang WL Wang YL Chang CT Wu YC Chao SH Kao A Yuan CW Lin SC Yang WK Chan KC Li TM Hong PC Yang 2009 p53 controls cancer cell invasion by inducing the MDM2-mediated degradation of Slug Nat Cell Biol 11 694 704 19448627 10.1038/ncb1875 1:CAS:528:DC%2BD1MXms1als7g%3D
13. UK Mukhopadhyay R Eves L Jia P Mooney AS Mak 2009 p53 suppresses Src-induced podosome and rosette formation and cellular invasiveness through the upregulation of caldesmon Mol Cell Biol 29 3088 3098 19349302 10.1128/MCB.01816-08 1:CAS:528:DC%2BD1MXmvVCqsb0%3D
14. NV Sankpal MW Willman TP Fleming JD Mayfield WE Gillanders 2009 Transcriptional repression of epithelial cell adhesion molecule contributes to p53 control of breast cancer invasion Cancer Res 69 753 757 19141643 10.1158/0008-5472.CAN-08-2708 1:CAS:528:DC%2BD1MXhtFOksLg%3D
15. N Moskovits A Kalinkovich J Bar T Lapidot M Oren 2006 p53 attenuates cancer cell migration and invasion through repression of SDF-1/CXCL12 expression in stromal fibroblasts Cancer Res 66 10671 10676 17108103 10.1158/0008-5472. CAN-06-2323 1:CAS:528:DC%2BD2sXivVyqs7s%3D (Pubitemid 44876961)
16. C Reynet CR Kahn 1993 Rad: a member of the Ras family overexpressed in muscle of type II diabetic humans Science 262 1441 1444 8248782 10.1126/science.8248782 1:CAS:528:DyaK2cXhtVersLY%3D (Pubitemid 24035065)
17. K Kelly 2005 The RGK family: a regulatory tail of small GTP-binding proteins Trends Cell Biol 15 640 643 16242932 10.1016/j.tcb.2005.10.002 1:CAS:528:DC%2BD2MXht1OntLjN (Pubitemid 41713964)
18. RN Mahalakshmi MY Ng K Guo Z Qi W Hunziker P Beguin 2007 Nuclear localization of endogenous RGK proteins and modulation of cell shape remodeling by regulated nuclear transport Traffic 8 1164 1178 17605760 10.1111/j.1600-0854. 2007.00599.x 1:CAS:528:DC%2BD2sXhtVeht7vP (Pubitemid 47248472)
19. Y Ward Y Seow-Fong V Ravichandran F Matsumura M Ito B Spinelli K Kelly 2002 The GTP binding proteins Gem and Rad are negative regulators of the Rho-Rho kinase pathway J Cell Biol 157 291 302 11956230 10.1083/jcb.200111026 1:CAS:528:DC%2BD38XivFKktLg%3D (Pubitemid 34839825)
20. E Saller E Tom M Brunori M Otter A Estreicher DH Mack R Iggo 1999 Increased apoptosis induction by 121F mutant p53 EMBO J 18 4424 4437 10449408 10.1093/emboj/18.16.4424 1:CAS:528:DyaK1MXlslWgu7k%3D (Pubitemid 29391252)
21. TR Brummelkamp R Bernards R Agami 2002 A system for stable expression of short interfering RNAs in mammalian cells Science 296 550 553 11910072 10.1126/science.1068999 1:CAS:528:DC%2BD38XjtFCjtrk%3D (Pubitemid 34408678)
22. PK Lo JY Chen PP Tang J Lin CH Lin LT Su CH Wu TL Chen Y Yang FF Wang 2001 Identification of a mouse thiamine transporter gene as a direct transcriptional target for p53 J Biol Chem 276 37186 37193 11481326 10.1074/jbc.M104701200 1:CAS:528:DC%2BD3MXns1elur4%3D
23. SC Teng YY Chen YN Su PC Chou YC Chiang SF Tseng KJ Wu 2004 Direct activation of HSP90A transcription by c-Myc contributes to c-Myc-induced transformation J Biol Chem 279 14649 14655 14724288 10.1074/jbc.M308842200 1:CAS:528:DC%2BD2cXivVKht70%3D (Pubitemid 38618853)
24. P Friedlander Y Haupt C Prives M Oren 1996 A mutant p53 that discriminates between p53-responsive genes cannot induce apoptosis Mol Cell Biol 16 4961 4971 8756655 1:CAS:528:DyaK28XltFKjtrc%3D (Pubitemid 26272151)
25. M Grunstein 1997 Histone acetylation in chromatin structure and transcription Nature 389 349 352 9311776 10.1038/38664 1:CAS:528: DyaK2sXmsVWntLk%3D (Pubitemid 27415209)
26. J Soosairajah S Maiti ON Wiggan P Sarmiere N Moussi B Sarcevic R Sampath JR Bamburg O Bernard 2005 Interplay between components of a novel LIM kinase-slingshot phosphatase complex regulates cofilin EMBO J 24 473 486 15660133 10.1038/sj.emboj.7600543 1:CAS:528:DC%2BD2MXhtFarur0%3D (Pubitemid 40343250)
27. M Fu J Zhang YH Tseng T Cui X Zhu Y Xiao Y Mou H De Leon MM Chang Y Hamamori CR Kahn YE Chen 2005 Rad GTPase attenuates vascular lesion formation by inhibition of vascular smooth muscle cell migration Circulation 111 1071 1077 15710763 10.1161/01.CIR.0000156439.55349.AD 1:CAS:528:DC%2BD2MXhsFShu78%3D (Pubitemid 40354527)
28. F Guo Y Zheng 2004 Rho family GTPases cooperate with p53 deletion to promote primary mouse embryonic fibroblast cell invasion Oncogene 23 5577 5585 15122327 10.1038/sj.onc.1207752 1:CAS:528:DC%2BD2cXlvVehsLk%3D (Pubitemid 39093035)
29. K Lefort A Mandinova P Ostano V Kolev V Calpini I Kolfschoten V Devgan J Lieb W Raffoul D Hohl V Neel J Garlick G Chiorino GP Dotto 2007 Notch1 is a p53 target gene involved in human keratinocyte tumor suppression through negative regulation of ROCK1/2 and MRCKalpha kinases Genes Dev 21 562 577 17344417 10.1101/gad.1484707 1:CAS:528:DC%2BD2sXjsVSqtb4%3D (Pubitemid 46409471)
30. A Petitjean E Mathe S Kato C Ishioka SV Tavtigian P Hainaut M Olivier 2007 Impact of mutant p53 functional properties on TP53 mutation patterns and tumor phenotype: lessons from recent developments in the IARC TP53 database Hum Mutat 28 622 629 17311302 10.1002/humu.20495 1:CAS:528:DC%2BD2sXmsVymur8%3D (Pubitemid 46744292)
31. M Suzuki H Shigematsu DS Shames N Sunaga T Takahashi N Shivapurkar T Iizasa JD Minna T Fujisawa AF Gazdar 2007 Methylation and gene silencing of the Ras-related GTPase gene in lung and breast cancers Ann Surg Oncol 14 1397 1404 17195088 10.1245/s10434-006-9089-6 (Pubitemid 46536799)
32. YH Tseng D Vicent J Zhu Y Niu A Adeyinka JS Moyers PH Watson CR Kahn 2001 Regulation of growth and tumorigenicity of breast cancer cells by the low molecular weight GTPase Rad and nm23 Cancer Res 61 2071 2079 11280768 1:CAS:528:DC%2BD3MXit1OgsL0%3D (Pubitemid 32692029)
33. SY Shieh M Ikeda Y Taya C Prives 1997 DNA damage-induced phosphorylation of p53 alleviates inhibition by MDM2 Cell 91 325 334 9363941 10.1016/S0092-8674(00)80416-X 1:CAS:528:DyaK2sXnt1CltLY%3D (Pubitemid 27467963)
34. WD Funk DT Pak RH Karas WE Wright JW Shay 1992 A transcriptionally active DNA-binding site for human p53 protein complexes Mol Cell Biol 12 2866 2871 1588974 1:CAS:528:DyaK3sXhsleltw%3D%3D