Nuclear localization of endogenous RGK proteins and modulation of cell shape remodeling by regulated nuclear transport

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Volumn 8, Issue 9, 2007, Pages 1164-1178

  Mahalakshmi, Ramasubbu N ^a   Ng, Mei Yong ^a   Guo, Ke ^a   Qi, Zeng ^a   Hunziker, Walter ^a   Béguin, Pascal ^a  

^a INSTITUTE OF MOLECULAR AND CELL BIOLOGY   (Singapore)

Author keywords

14 3 3; Cell shape; GTP binding protein; Importin; Nuclear; Nuclear localization; Phosphorylation

Indexed keywords

GUANINE NUCLEOTIDE BINDING PROTEIN; KARYOPHERIN; KARYOPHERIN ALPHA3; KARYOPHERIN ALPHA5; KARYOPHERIN BETA; PROTEIN 14 3 3; PROTEIN GEM; PROTEIN KIR; PROTEIN RAD; PROTEIN REM; PROTEIN REM2; SERINE; UNCLASSIFIED DRUG;

ANIMAL CELL; ARTICLE; BRAIN CORTEX; BRAIN NERVE CELL; CELL NUCLEUS; CELL SHAPE; CELLULAR DISTRIBUTION; CONTROLLED STUDY; CYTOPLASM; CYTOSKELETON; CYTOSOL; EMBRYO; ENDOPLASMIC RETICULUM; HIPPOCAMPUS; HUMAN; HUMAN CELL; MOUSE; NERVE CELL MEMBRANE; NONHUMAN; NUCLEAR LOCALIZATION SIGNAL; PREDICTION; PRIORITY JOURNAL; PROTEIN FUNCTION; PROTEIN LOCALIZATION; PROTEIN PHOSPHORYLATION; PROTEIN PROTEIN INTERACTION; PROTEIN TRANSPORT; RAT;

14-3-3 PROTEINS; ACTIVE TRANSPORT, CELL NUCLEUS; ANIMALS; CELL LINE, TUMOR; CELL NUCLEUS; CELL SHAPE; CELLS, CULTURED; CERAMIDES; CERCOPITHECUS AETHIOPS; COS CELLS; HELA CELLS; HUMANS; KARYOPHERINS; MICE; MODELS, BIOLOGICAL; MONOMERIC GTP-BINDING PROTEINS; NEURONS; NIH 3T3 CELLS; NUCLEAR LOCALIZATION SIGNALS; PHOSPHORYLATION; PROTEIN BINDING; PROTEIN PROCESSING, POST-TRANSLATIONAL; RAS PROTEINS; RATS;

EID: 34547906490     PISSN: 13989219     EISSN: 16000854     Source Type: Journal    
DOI: 10.1111/j.1600-0854.2007.00599.x     Document Type: Article

References (32)

1. Reynet C, Kahn CR. Rad: A member of the Ras family overexpressed in muscle of type II diabetic humans. Science 1993;262:1441-1444.
2. Finlin BS, Andres DA. Rem is a new member of the Rad- and Gem/Kir Ras-related GTP-binding protein family repressed by lipopolysaccharide stimulation. J Biol Chem 1997;272:21982-21988.
3. Finlin BS, Shao H, Kadono-Okuda K, Guo N, Andres DA. Rem2, a new member of the Rem/Rad/Gem/Kir family of Ras-related GTPases. Biochem J 2000;347:223-231.
4. Béguin P, Mahalakshmi RN, Nagashima K, Cher DH, Kuwamura N, Yamada Y, Seino Y, Hunziker W. Roles of 14-3-3 and calmodulin binding in subcellular localization and function of the small G-protein Rem2. Biochem J 2005;390:67-75.
5. Maguire J, Santoro T, Jensen P, Siebenlist U, Yewdell J, Kelly K. Gem: an induced, immediate early protein belonging to the Ras family. Science 1994;265:241-244.
6. Cohen L, Mohr R, Chen YY, Huang M, Kato R, Dorin D, Tamanoi F, Goga A, Afar D, Rosenberg N, Witte O. Transcriptional activation of a ras-like gene (kir) by oncogenic tyrosine kinases. Proc Natl Acad Sci U S A 1994;91:12448-12452.
7. Moyers JS, Bilan PJ, Reynet C, Kahn CR. Overexpression of Rad inhibits glucose uptake in cultured muscle and fat cells. J Biol Chem 1996;271:23111-23116.
8. Béguin P, Mahalakshmi RN, Nagashima K, Cher DH, Ikeda H, Yamada Y, Seino Y, Hunziker W. Nuclear sequestration of beta-subunits by Rad and Rem is controlled by 14-3-3 and calmodulin and reveals a novel mechanism for Ca2+ channel regulation. J Mol Biol 2006;355:34-46.
9. Béguin P, Mahalakshmi RN, Nagashima K, Cher DH, Takahashi A, Yamada Y, Seino Y, Hunziker W. 14-3-3 and calmodulin control subcellular distribution of Kir/Gem and its regulation of cell shape and calcium channel activity. J Cell Sci 2005;118:1923-1934.
10. Béguin P, Nagashima K, Gonoi T, Shibasaki T, Takahashi K, Kashima Y, Ozaki N, Geering K, Iwanaga T, Seino S. Regulation of Ca2+ channel expression at the cell surface by the small G-protein kir/Gem. Nature 2001;411:701-706.
11. Kelly K. The RGK family: A regulatory tail of small GTP-binding proteins. Trends Cell Biol 2005;15:640-643.
12. Splingard A, Menetrey J, Perderiset M, Cicolari J, Regazzoni K, Hamoudi F, Cabanie L, El Marjou A, Wells A, Houdusse A, de Gunzburg J. Biochemical and structural characterization of the gem GTPase. J Biol Chem 2007;282:1905-1915.
13. Finlin BS, Crump SM, Satin J, Andres DA. Regulation of voltage-gated calcium channel activity by the Rem and Rad GTPases. Proc Natl Acad Sci U S A 2003;100:14469-14474.
14. Finlin BS, Mosley AL, Crump SM, Correll RN, Ozcan S, Satin J, Andres DA. Regulation of L-type Ca2+ channel activity and insulin secretion by the Rem2 GTPase. J Biol Chem 2005;280:41864-41871.
15. Ward Y, Spinelli B, Quon MJ, Chen H, Ikeda SR, Kelly K. Phosphorylation of critical serine residues in Gem separates cytoskeletal reorganization from down-regulation of calcium channel activity. Mol Cell Biol 2004;24:651-661.
16. Chen H, Puhl HL III, Niu SL, Mitchell DC, Ikeda SR. Expression of Rem2, an RGK family small GTPase, reduces N-type calcium current without affecting channel surface density. J Neurosci 2005;25:9762-9772.
17. Murata M, Cingolani E, McDonald AD, Donahue JK, Marban E. Creation of a genetic calcium channel blocker by targeted gem gene transfer in the heart. Circ Res 2004;95:398-405.
18. Beguin P, Ng YJ, Krause C, Mahalakshmi RN, Ng MY, Hunziker W. RGK small GTP-binding proteins interact with the nucleotide kinase domain of Ca2+-channel beta-subunits via an uncommon effector binding domain. J Biol Chem 2007;282:11509-11520.
19. Pan JY, Fieles WE, White AM, Egerton MM, Silberstein DS. Ges, a human GTPase of the Rad/Gem/Kir family, promotes endothelial cell sprouting and cytoskeleton reorganization. J Cell Biol 2000;149:1107-1116.
20. Piddini E, Schmid JA, de Martin R, Dotti CG. The Ras-like GTPase Gem is involved in cell shape remodelling and interacts with the novel kinesin-like protein KIF9. EMBO J 2001;20:4076-4087.
21. Leone A, Mitsiades N, Ward Y, Spinelli B, Poulaki V, Tsokos M, Kelly K. The Gem GTP-binding protein promotes morphological differentiation in neuroblastoma. Oncogene 2001;20:3217-3225.
22. Ward Y, Yap SF, Ravichandran V, Matsumura F, Ito M, Spinelli B, Kelly K. The GTP binding proteins Gem and Rad are negative regulators of the Rho-Rho kinase pathway. J Cell Biol 2002;157:291-302.
23. Hatzoglou A, Ader I, Splingard A, Flanders J, Saade E, Leroy I, Traver S, Aresta S, de Gunzburg J. Gem associates with Ezrin and acts via the Rho-GAP protein Gmip to down-regulate the Rho pathway. Mol Biol Cell 2007;18:1242-1252.
24. Mackintosh C. Dynamic interactions between 14-3-3 proteins and phosphoproteins regulate diverse cellular processes. Biochem J 2004;381:329-342.
25. Vetter SW, Leclerc E. Novel aspects of calmodulin target recognition and activation. Eur J Biochem 2003;270:404-414.
26. Chalfant CE, Kishikawa K, Mumby MC, Kamibayashi C, Bielawska A, Hannun YA. Long chain ceramides activate protein phosphatase-1 and protein phosphatase-2A. Activation is stereospecific and regulated by phosphatidic acid. J Biol Chem 1999;274:20313-20317.
27. Sasaki T, Shibasaki T, Beguin P, Nagashima K, Miyazaki M, Seino S. Direct inhibition of the interaction between {alpha}-interaction domain and {beta}-interaction domain of voltage-dependent Ca2+ channels by Gem. J Biol Chem 2005;280:9308-9312.
28. Yang W, Musser SM. Nuclear import time and transport efficiency depend on importin {beta} concentration. J Cell Biol 2006;174:951-961.
29. Opatowsky Y, Chomsky-Hecht O, Kang MG, Campbell KP, Hirsch JA. The voltage-dependent calcium channel beta subunit contains two stable interacting domains. J Biol Chem 2003;278:52323-52332.
30. Smith DB, Johnson KS. Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase. Gene 1988;67:31-40.
31. Quensel C, Friedrich B, Sommer T, Hartmann E, Kohler M. In vivo analysis of importin alpha proteins reveals cellular proliferation inhibition and substrate specificity. Mol Cell Biol 2004;24:10246-10255.
32. Mahalakshmi RN, Nagashima K, Ng MY, Inagaki N, Hunziker W, Béguin P. Nuclear transport of Kir/Gem requires specific signals and importin α5 and is regulated by Calmodulin and predicted service phosphorylations. Traffic 2007; doi: 10.1111/j.1600-0854.2007.00598.x.