Kinetic analysis of unpurified native antigens available in very low quantities and concentrations

Analytical Biochemistry

Volumn 414, Issue 1, 2011, Pages 7-13

  Rathanaswami, Palaniswami ^a   Richmond, Karen ^a   Manchulenko, Kathy ^a   Foltz, Ian N ^a  

^a Amgen British Columbia   (Canada)

Author keywords

Affinity measurement; Anti human IL13; Equilibrium binding measurement; IL13; Kinetic analysis; Kinetic exclusion assay (KinExA); Monoclonal antibodies; Native antigens; Native molecules

Indexed keywords

CELL CULTURE; KINETICS; MONOCLONAL ANTIBODIES; PURIFICATION;

AFFINITY MEASUREMENT; ANTI-HUMAN IL13; EQUILIBRIUM BINDING; IL13; KINETIC ANALYSIS;

ANTIGENS;

ANTIGEN; INTERLEUKIN 13 ANTIBODY; MONOCLONAL ANTIBODY;

ARTICLE; HUMAN; KINETIC EXCLUSION ASSAY; KINETICS; MEASUREMENT; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN DETERMINATION; T LYMPHOCYTE;

EID: 79955697298     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2011.02.034     Document Type: Article

References (16)

1. P. Rathanaswami, S. Roalstad, L. Roskos, Q.J. Su, S. Lackie, J. Babcook, Demonstration of an in vivo generated sub-picomolar affinity fully human monoclonal antibody to interleukin-8, Biochem. Biophys. Res. Commun. 334 (2005) 1004-1013. (Pubitemid 41074676)
2. L.S. Zuckier, E.Z. Berkowitz, R.J. Sattenberg, Q.H. Zhao, H.F. Deng, M.D. Scharff, Influence of affinity and antigen density on antibody localization in a modifiable tumor targeting model, Cancer Res. 60 (2000) 7008-7013. (Pubitemid 32059177)
3. S.J. Kennel, L.J. Foote, P.K. Lankford, M. Johnson, T. Mitchell, G.R. Braslawsky, Direct binding of radioiodinated monoclonal antibody to tumor cells: significance of antibody purity and affinity for drug targeting or tumor imaging, Hybridoma 2 (1983) 297-310. (Pubitemid 14176978)
4. Y.N. Abdiche, D.S. Malashock, J. Pons, Probing the binding mechanism and affinity of tanezumab, a recombinant humanized anti-NGF monoclonal antibody, using a repertoire of biosensors, Protein Sci. 17 (2008) 1326-1335.
5. R.J. Falconer, B.M. Collins, Survey of the year 2009: applications of isothermal titration calorimetry, J. Mol. Recognit. 24 (2011) 1-16.
6. R.J. Darling, P.A. Brault, Kinetic exclusion assay technology: characterization of molecular interactions, Assay Drug Dev. Technol. 2 (2004) 647-657.
7. P. Rathanaswami, J. Babcook, M. Gallo, High-affinity binding measurements of antibodies to cell-surface-expressed antigens, Anal. Biochem. 373 (2008) 52-60.
8. A. Razai, C. Garcia-Rodriguez, J. Lou, I.N. Geren, C.M. Forsyth, Y. Robles, R. Tsai, T.J. Smith, L.A. Smith, R.W. Siegel, M. Feldhaus, J.D. Marks, Molecular evolution of antibody affinity for sensitive detection of botulinum neurotoxin type A, J. Mol. Biol. 351 (2005) 158-169.
9. L.L. Green, M.C. Hardy, C.E. Maynard-Currie, H. Tsuda, D.M. Louie, M.J. Mendez, H. Abderrahim, M. Noguchi, D.H. Smith, Y. Zeng, et al., Antigen-specific human monoclonal antibodies from mice engineered with human Ig heavy and light chain YACs, Nat. Genet. 7 (1994) 13-21.
10. J.M. Reichert, Monoclonal antibodies as innovative therapeutics, Curr. Pharm. Biotechnol. 9 (2008) 423-430.
11. J.H. Anolik, M. Aringer, New treatments for SLE: cell-depleting and anticytokine therapies, Best Pract. Res. Clin. Rheumatol. 19 (2005) 859-878. (Pubitemid 41258587)
12. L. Yan, G.M. Anderson, M. DeWitte, M.T. Nakada, Therapeutic potential of cytokine and chemokine antagonists in cancer therapy, Eur. J. Cancer 42 (2006) 793-802.
13. D. Zagury, R.C. Gallo, Anti-cytokine Ab immune therapy: present status and perspectives, Drug Discov. Today 9 (2004) 72-81. (Pubitemid 38169531)
14. J.E. de Vries, The role of IL-13 and its receptor in allergy and inflammatory responses, J. Allergy Clin. Immunol. 102 (1998) 165-169.
15. G. Grunig, M. Warnock, A.E. Wakil, R. Venkayya, F. Brombacher, D.M. Rennick, D. Sheppard, M. Mohrs, D.D. Donaldson, R.M. Locksley, D.B. Corry, Requirement for IL-13 independently of IL-4 in experimental asthma, Science 282 (1998) 2261-2263. (Pubitemid 29004075)
16. L. Xie, R. Mark Jones, T.R. Glass, R. Navoa, Y. Wang, M.J. Grace, Measurement of the functional affinity constant of a monoclonal antibody for cell surface receptors using kinetic exclusion fluorescence immunoassay, J. Immunol. Methods 304 (2005) 1-14. (Pubitemid 41262216)