Vancomycin-resistant Enterococcus faecium isolates causing hospital outbreaks in northern Italy belong to the multilocus sequence typing C1 lineage

Microbial Drug Resistance

Volumn 10, Issue 2, 2004, Pages 114-123

  Bonora, Maria Grazia ^a   Ligozzi, Marco ^a   De Fatima, Maria ^a   Bragagnolo, Luciana ^b   Goglio, Antonio ^c   Guazzotti, Gian Carlo ^d   Fontana, Roberta ^a  

^a UNIVERSITY OF VERONA   (Italy)

^b SAN BORTOLO HOSPITAL   (Italy)

^c OSPEDALI RIUNITI   (Italy)

^d SANT'ANDREA HOSPITAL   (Italy)

Author keywords

[No Author keywords available]

Indexed keywords

ABC TRANSPORTER; ADENYLATE KINASE; DEXTRO ALANINE DEXTRO ALANINE LIGASE; GLUCOSE 6 PHOSPHATE DEHYDROGENASE; GLYCERALDEHYDE 3 PHOSPHATE DEHYDROGENASE; PROTON TRANSPORTING ADENOSINE TRIPHOSPHATE SYNTHASE; VANCOMYCIN;

ADK GENE; ANTIBIOTIC RESISTANCE; ARTICLE; ATPA GENE; BACTERIAL GENE; BACTERIUM ISOLATION; CELL LINEAGE; DDL GENE; ENTEROCOCCUS FAECIUM; EPIDEMIC; GDH GENE; GENE FREQUENCY; GENE LOCUS; GYD GENE; HOSPITAL INFECTION; HOUSEKEEPING GENE; ITALY; NONHUMAN; PHYLOGENETIC TREE; PRIORITY JOURNAL; PSTS GENE; PURK GENE; SOUTHERN BLOTTING;

ALLELES; ANIMALS; BASE SEQUENCE; DISEASE OUTBREAKS; DNA, BACTERIAL; DRUG RESISTANCE, BACTERIAL; ELECTROPHORESIS, GEL, PULSED-FIELD; ENTEROCOCCUS FAECIUM; FOOD MICROBIOLOGY; GRAM-POSITIVE BACTERIAL INFECTIONS; HUMANS; ITALY; MEAT; PHYLOGENY; POLYMORPHISM, GENETIC; POLYMORPHISM, SINGLE NUCLEOTIDE; POULTRY; SEROTYPING; TIME FACTORS; VANCOMYCIN;

BACTERIA (MICROORGANISMS); ENTEROCOCCUS; ENTEROCOCCUS FAECIUM;

EID: 3042776748     PISSN: 10766294     EISSN: None     Source Type: Journal    
DOI: 10.1089/1076629041310046     Document Type: Article

References (37)

1. Aarestrup, F.M. 2000. Characterization of glycopeptide-resistant Enterococcus faecium (GRE) from broilers and pigs in Denmark: genetic evidence that persistence of GRE in pig herds is associated with coselection by resistance to macrolides. J. Clin. Microbiol. 38:2774-2777.
2. Bertrand, X., B. Mulin, J.F. Viel, M. Thouverez, and D. Talon. 2000. Common PFGE patterns in antibiotic-resistant Enterococcus faecalis from humans and cheeses. Food Microbiol. 17:543-551.
3. Biavasco, F., E. Giovanetti, A. Miele, C. Vignaroli, B. Facinelli, and P.E. Varaldo. 1996. Genotypic characterization of a nosocomial outbreak of VanA Enterococcus faecalis. Microb. Drug Resist. 2:231-237.
4. Bonadio, M., M. Meini, E. Tagliaferri, C. Gigli, and A. Vigna. 2000. Enterococcal glycopeptide resistance at an Italian teaching hospital. J. Antimicrob. Chemother. 46:129-131.
5. Bonora, M.G., C. Bordin, L. Bragagnolo, L. Cirelli, M. De Fatima, A. Grossato, M. Ligozzi, G. Lo Cascio, and R. Fontana. 2001. Molecular analysis of vanA enterococci isolated from humans and animals in the North East of Italy. Microb. Drug Resist. 7:247-256.
6. Del Campo, R., C. Tenorio, M. Zarazaga, R. Gomez-Lus, F. Baquero, and C. Torres. 2001. Detection of a single vanA-containing Enterococcus faecalis clone in hospitals in different regions in Spain. J. Antimicrob. Chemother. 48:746-747.
7. Dutka-Malen, S., S. Evers, and P. Courvalin. 1995. Detection of glycopeptide resistance genotypes and identification to the species level of clinically relevant enterococci by PCR. J. Clin. Microbiol. 33:24-27.
8. Eaton, T.J., and M.J. Gasson. 2001. Molecular screening of Enterococcus virulence determinants and potential for genetic exchange between food and medical isolates. Appl. Enviromen. Microbiol. 67:1628-1635.
9. Enright, M.C., and B.G. Spratt. 1998. A multilocus sequence typing scheme for Streptococcus pneumoniae: identification of clones associated with serious invasive disease. Microbiology. 144:3049-3060.
10. Enright, M.C., and B.G. Spratt. 1999. Multilocus sequence typing. Trends Microbiol. 7:482-487.
11. Enright, M.C., B.G. Spratt, A. Kalia, J.H. Cross, and D.E. Bessen. 2001. Multilocus sequence typing of Streptococcus pyogenes and the relationships between emm type and clone. Infect. Immun. 69:2416-2427.
12. Enright, M.C., N.P. Day, C.E. Davies, S.J. Peacock, and B.G. Spratt. 2000. Multilocus sequence typing for characterization of methicillin-resistant and methicillin-susceptible clones of Staphylococcus aureus. J. Clin. Microbiol. 38:1008-1015.
13. Fontana, R., M. Ligozzi, A. Mazzariol, G. Veneri, and G. Cornaglia. 1988. Resistance of enterococci to ampycillin and glycopeptide antibiotics in Italy. The Italian Surveillance Group for Antimicrobial Resistance. Clin. Infect. Dis. 27 (Suppl.1):S84-86.
14. Gambarotto, K., M.C. Ploy, P. Turlure, C. Grélaud, C. Martin, D. Bordessoule, and F. Denis. 2000. Prevalence of vancomycin-resistant enterococci in fecal samples from hospitalized patients and nonhospitalized controls in a cattle-rearing area of France. J. Clin. Microbiol. 38:620-624.
15. Giraffa, G., A.M. Olivari, and E. Neviani. 2000. Isolation of vancomycin-resistant Enterococcus faecium from Italian cheeses. Food Microbiol. 17:671-677.
16. Homan, W.L., D. Tribe, S. Poznanski, M. Li, G. Hogg, E. Spalburg, J. Van Embden, and R.J.L. Willems. 2002. Multilocus sequence typing scheme for Enterococcus faecium. J. Clin. Microbiol. 40:1963-1971.
17. Klare, I., H. Heier, H. Claus, G. Bohme, S. Marin, G. Seltmann, R. Hakenbeck, V. Antanassova, and W. Witte. 1995. E. faecium strains with vanA-mediated high-level glycopeptide resistance isolated from animals foodstuffs and fecal samples of humans in the community. Microb. Drug Resist. 1:265-272.
18. Maiden, M.C., J.A. Bygraves, E. Feil, G. Morelli, J.E. Russel, R. Urwin, Q. Zhang, J. Zhou, K. Zurth, D.A. Caugant, I.M. Feavers, M. Achtman, and B.G. Spratt. 1998. Multilocus sequence typing: a portable approach to the identification of clones within populations of pathogenic microorganisms. Proc. Natl. Acad. Sci. USA 95:3140-3145.
19. Manso, E., G. De Sio, F. Biavasco, and P.E. Varaldo. 1993. Vancomycin-resistant enterococci. Lancet 342:616-617.
20. Mundy, L.M., D.F. Sahm, and M. Gilmore. 2000. Relationships between enterococcal virulence and antimicrobial resistance. Clin. Microbiol. Rev. 13:513-522.
21. Murray, B.E., K.V. Singh, J.D. Heath, B.R. Sharma, and G.M. Weinstock. 1990. Comparison of genomic DNAs of different enterococcal isolates using restriction endonucleases with infrequent recognition sites. J. Clin. Microbiol. 28:2059-2063.
22. Nallapareddy, S.R., R.W. Duh, K.V. Singh, and B.E. Murray. 2002. Molecular typing of selected Enterococcus faecalis isolates: pilot study using multilocus sequence typing and pulsed-field gel electrophoresis. J. Clin. Microbiol. 40:868-876.
23. National Committee for Clinical Laboratory Standards. 2002. Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically. Approved standard M7-A5. National Committee for Clinical Laboratory Standards, Wayne, PA.
24. Pegues, D.A., C.F. Pegues, P.L. Hibberd, D.S. Ford, and D.C. Hooper. 1997. Emergence and dissemination of a highly vancomycin-resistant vanA strain of Enterococcus faecium at a large teaching hospital. J. Clin. Microbiol. 35:1565-1570.
25. Sader, H.S., M.A. Pfaller, F.C. Tenover, R.J. Hollis, and R.N. Jones. 1994. Evaluation and characterization of multiresistant Enterococcus faecium from 12 U.S. Medical Centers. 32:2840-2842.
26. Scagnelli, M., G. Pellizer, F. De Lalla, A. D'Emilio, M. Rassu, L. Bragagnolo, P. Reatto, G. Veneri, M. Ligozzi, and R. Fontana. 2001. Epidemiological analysis of vancomycin-resistant enterococci in a large tertiary-care hospital in Northern Italy. Eur. J. Clin. Microbiol. Infect. Dis. 20:609-616.
27. Tahl, L., S. Donabedian, B. Robinson-Dunn, J.W. Chow, L. Dembry, D.B. Clewell, D. Alshab, and M.J. Zervos. 1998. Molecular analysis of glycopeptide-resistant Enterococcus faecium isolates collected from Michigan hospitals over a 6-year period. J. Clin. Microbiol. 36:3303-3308.
28. Tenover, F.C., R.V. Arbeit, R.V. Goering, P.A. Mickelsen, B.E. Murray, D.H. Persing, and B. Swaminathan. 1995. Interpreting chromosomal DNA restriction patterns produced by pulsed-field gel electrophoresis: criteria for bacterial strain typing. J. Clin. Microbiol. 33:2233-2239.
29. Thompson, J.D., D.G. Higgins, and T.J. Gibson. 1994. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res. 22:4673-4680.
30. Van der Auwera, P., N. Pensart, V. Korten, B.E. Murray, and R. Leclerq. 1996. Influence of oral glycopeptides on the fecal flora of human volunteers: selection of highly glycopeptide-resistant enterococci. J. Infect. Dis. 173:1129-1136.
31. Van den Bogaard, A.E., L.B. Jensen, and E.E. Stobberingh. 1997. Vancomycin-resistant enterococci in turkeys and farmers. N. Engl. J. Med. 337:1558-1559.
32. Van den Braak, N., A. van Belkum, M. van Keulen, J. Vliegenthart, H.A. Verbrugh, and H.P. Endtz. 1998. Molecular characterization of vancomycin-resistant enterococci from hospitalized patients and poultry products in The Netherlands. J. Clin. Microbiol. 36:1927-1932.
33. Werner, G., I. Klare, and W. Witte. 1997. Arrangement of the vanA gene cluster in enterococci of different ecological origin. FEMS Microbiol. Lett. 155:55-61.
34. Willems, R.J.L., J. Top, N. van der Braak, A. van Belkum, D.J. Mevious, G. Henfriks, M. van Santen-Verheuvel, and J.D.A. van Embden. 1999. Molecular diversity and evolutionary relationship of Tn1546-like elements in enterococci from humans and animals. Antimicrob. Agents Chemother. 43:483-491.
35. Willems, R.J.L., J. Top, N. van der Braak, A. van Belkum, H. Endtz, D.J. Mevious, E. Stobberingh, A. van den Bogaard, and J.D.A. van Embden. 2000. Host specificity of vancomycin-resistant Enterococcus faecium. J. Infect. Dis. 182:816-823
36. Willems, R.J.L., H. Wieger, J. Top, N. van der Braak, M. van Santen-Verheuel, D. Tribe, X. Manzioros, C. Gaillard, C.M. Vandenbroucke, E.M. Mascini, E. van Kregten, J.D. van Embden, and M.J. Bonten. 2001. Variant esp gene as a marker of a distinct genetic lineage of vancomycin-resistant Enterococcus faecium spreading in hospitals. The Lancet 357:853-855.
37. Woodford, N., A.A. Adebiyi, M.I. Palepou, and B.D. Cookson. 1998. Diversity of vanA glycopeptide resistance elements in enterococci from humans and nonhuman sources. Antimicrob. Agents Chemother. 42:502-508.