Thermodynamic analysis of mutant lac repressors

Journal of Molecular Biology

Volumn 409, Issue 1, 2011, Pages 76-87

  Daber, Robert ^a   Sochor, Matthew A ^a   Lewis, Mitchell ^a  

^a UNIVERSITY OF PENNSYLVANIA   (United States)

Author keywords

allostery; gene regulation; induction; lac repressor

Indexed keywords

LAC REPRESSOR; MUTANT PROTEIN;

ARTICLE; GENE EXPRESSION REGULATION; GENE SWITCHING; PHENOTYPIC VARIATION; PRIORITY JOURNAL; PROTEIN DNA BINDING; PROTEIN DOMAIN; PROTEIN EXPRESSION; THERMODYNAMICS; TRANSCRIPTION REGULATION;

BACTERIA (MICROORGANISMS);

EID: 79955601418     PISSN: 00222836     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jmb.2011.03.057     Document Type: Article

References (19)

1. Monod J., Changeux J.P., and Jacob F. Allosteric proteins and cellular control systems J. Mol. Biol. 6 1963 306 329
2. Monod J., Wyman J., and Changeux J.P. On the nature of allosteric transitions: a plausible model J. Mol. Biol. 12 1965 88 118
3. Jobe A., and Bourgeois S. Lac repressor-operator interaction: VIII. Lactose is an anti-inducer of the lac operon J. Mol. Biol. 75 1973 303 313
4. Lewis M., Chang G., Horton N.C., Kercher M.A., Pace H.C., and Schumacher M.A. Crystal structure of the lactose operon repressor and its complexes with DNA and inducer [see comment] Science 271 1996 1247 1254
5. Boelens R., Scheek R.M., van Boom J.H., and Kaptein R. Complex of lac repressor headpiece with a 14 base-pair lac operator fragment studied by two-dimensional nuclear magnetic resonance J. Mol. Biol. 193 1987 213 216
6. Wilson C.J., Zhan H., Swint-Kruse L., and Matthews K.S. The lactose repressor system: paradigms for regulation, allosteric behavior and protein folding Cell. Mol. Life Sci. 64 2007 3 16 (Pubitemid 46164491)
7. Swint-Kruse L., and Matthews K.S. Allostery in the LacI/GalR family: variations on a theme Curr. Opin. Microbiol. 12 2009 129 137
8. Daber R., and Lewis M. Towards evolving a better repressor Protein Eng. Des. Sel. 22 2009 673 683
9. Markiewicz P., Kleina L.G., Cruz C., Ehret S., and Miller J.H. Genetic studies of the lac repressor: XIV. Analysis of 4000 altered Escherichia coli lac repressors reveals essential and non-essential residues, as well as "spacers" which do not require a specific sequence J. Mol. Biol. 240 1994 421 433 (Pubitemid 24249917)
10. Kleina L.G., and Miller J.H. Genetic studies of the lac repressor: XIII. Extensive amino acid replacements generated by the use of natural and synthetic nonsense suppressors J. Mol. Biol. 212 1990 295 318 (Pubitemid 20130729)
11. Lehming N., Sartorius J., Kisters-Woike B., von Wilcken-Bergmann B., and Muller-Hill B. Mutant lac repressors with new specificities hint at rules for protein-DNA recognition.[erratum appears in EMBO J. 1990 May;9(5):1674] EMBO J. 9 1990 615 621 (Pubitemid 120034218)
12. Lehming N., Sartorius J., Niemoller M., Genenger G., v Wilcken-Bergmann B., and Muller-Hill B. The interaction of the recognition helix of lac repressor with lac operator EMBO J. 6 1987 3145 3153
13. Barkley M.D., Riggs A.D., Jobe A., and Bourgeois S. Interaction of effecting ligands with lac repressor and repressor-operator complex Biochemistry 14 1975 1700
14. Daber R., Stayrook S., Rosenberg A., and Lewis M. Structural analysis of lac repressor bound to allosteric effectors J. Mol. Biol. 370 2007 609 619 (Pubitemid 46879767)
15. Weickert M.J., and Adhya S. A family of bacterial regulators homologous to Gal and Lac repressors J. Biol. Chem. 267 1992 15869 15874
16. Chang W.I., and Matthews K.S. Role of Asp274 in lac repressor: diminished sugar binding and altered conformational effects in mutants Biochemistry 34 1995 9227 9234
17. Satya Lakshmi O., and Rao N.M. Evolving Lac repressor for enhanced inducibility Protein Eng. Des. Sel. 22 2009 53 58
18. Daber R., and Lewis M. A novel molecular switch J. Mol. Biol. 391 2009 661 670
19. Daber R., Sharp K., and Lewis M. One is not enough J. Mol. Biol. 392 2009 1133 1144