Construction of a fully active Cys-less elongation factor Tu: Functional role of conserved cysteine 81

Biochimica et Biophysica Acta - Proteins and Proteomics

Volumn 1814, Issue 5, 2011, Pages 684-692

  De Laurentiis, Evelina Ines ^a   Mo, Fan ^a   Wieden, Hans Joachim ^a  

^a UNIVERSITY OF LETHBRIDGE   (Canada)

Author keywords

Cysteine free; Elongation factor Tu; Evolution; Fluorescence; Protein design; Rapid kinetics

Indexed keywords

ALANINE; CYSTEINE 81; CYSTEINE DERIVATIVE; ELONGATION FACTOR TU; METHIONINE; SERINE; TRANSFER RNA; UNCLASSIFIED DRUG;

AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; ARTICLE; BIOCHEMISTRY; DISSOCIATION; HYDROLYSIS; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN PURIFICATION; RIBOSOME; RNA BINDING; SITE DIRECTED MUTAGENESIS;

AMINO ACID SEQUENCE; CYSTEINE; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; PEPTIDE ELONGATION FACTOR TU; PROTEIN STRUCTURE, SECONDARY; SEQUENCE HOMOLOGY, AMINO ACID; STRUCTURE-ACTIVITY RELATIONSHIP;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

EID: 79955590745     PISSN: 15709639     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.bbapap.2011.02.007     Document Type: Article

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