Cytotoxic and PPARs transcriptional activities of sterols from the Vietnamese soft coral Lobophytum laevigatum

Bioorganic and Medicinal Chemistry Letters

Volumn 21, Issue 10, 2011, Pages 2845-2849

  Quang, Tran Hong ^a,b   Ha, Tran Thu ^b   Minh, Chau Van ^b   Kiem, Phan Van ^b   Huong, Hoang Thanh ^b   Ngan, Nguyen Thi Thanh ^a   Nhiem, Nguyen Xuan ^a,b   Tung, Nguyen Huu ^a,b   Thao, Nguyen Phuong ^b   Thuy, Dinh Thi Thu ^b   Song, Seok Bean ^a   Boo, Hye Jin ^c   Kang, Hee Kyoung ^c   Kim, Young Ho ^a  

^a Chungnam National University   (South Korea)

^b GRADUATE UNIVERSITY OF SCIENCE AND TECHNOLOGY   (Viet Nam)

^c Jeju National University School of Medicine   (South Korea)

Author keywords

Alcyoniidae; Cytotoxic; Lobophytosterol; Lobophytum laevigatum; PPARs transcriptional; Soft coral

Indexed keywords

24 METHYL 22,25 EPOXYFUROST 5 ENE 3BETA,20BETA DIOL; 24 METHYLCHOLEST 5 ENE 3BETA,25 DIOL; CHOLESTEROL; ERGOST 5 ENE 3BETA,7ALPHA DIOL; LOBOPHYTOSTEROL; MITOXANTRONE; PEROXISOME PROLIFERATOR ACTIVATED RECEPTOR ALPHA; PEROXISOME PROLIFERATOR ACTIVATED RECEPTOR DELTA; PEROXISOME PROLIFERATOR ACTIVATED RECEPTOR GAMMA; PREGNENOLONE; STEROL; UNCLASSIFIED DRUG;

APOPTOSIS; ARTICLE; CANCER INHIBITION; CELL STRAIN HCT116; CHOLESTEROL METABOLISM; CHROMATIN CONDENSATION; CONCENTRATION RESPONSE; CONTROLLED STUDY; CORAL; CRYSTAL STRUCTURE; CYTOTOXICITY; DRUG ISOLATION; DRUG STRUCTURE; GLUCOSE METABOLISM; HUMAN; HUMAN CELL; IC 50; LIPID METABOLISM; LOBOPHYTUM LAEVIGATUM; SPECTROSCOPY; STEREOCHEMISTRY; TRANSCRIPTION INITIATION; UPREGULATION;

ANIMALS; ANTHOZOA; CELL PROLIFERATION; GENE EXPRESSION REGULATION; HCT116 CELLS; HUMANS; INHIBITORY CONCENTRATION 50; MAGNETIC RESONANCE SPECTROSCOPY; METHANOL; MODELS, MOLECULAR; MOLECULAR STRUCTURE; PEROXISOME PROLIFERATOR-ACTIVATED RECEPTORS; STEROLS;

ALCYONACEA; ALCYONIIDAE; LOBOPHYTUM;

EID: 79955550405     PISSN: 0960894X     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.bmcl.2011.03.089     Document Type: Article

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28. 50 of compound 1 for 24 h. Cells were washed with PBS prior to stained with 1 mg/mL DNA-specific fluorescent dye Hoechst 33342 for 30 min at 37 °C. Apoptotic bodies, with condensed and fragmented nuclei, were imaged using an inverted fluorescent microscope equipped with an IX-71 Olympus camera (magnification ×200).
29. 5 cells per well in 12-well plates and grown for 24 h prior to transfection. An optimized amount of DNA plasmid (0.5 μg PPRE-Luc and 0.2 μg CMV-PPARγ) was diluted in 100 μL Dulbecco's modified Eagle medium (DMEM). PLUS™ Reagent (0.5 μL) and 1 μL Lipofectamine™ LTX (Invitrogen, Carlsbad, CA) were then added to the DNA plasmid solution and mixed thoroughly. Following 30 min of incubation at room temperature, 100 μL of transfection mixture was added to the cells and mixed gently. After 24 h of transfection, the medium was replace with Opti-MEM (Invitrogen, Carlsbad, CA) containing 0.1 mM Non-Essential Amino Acids, 0.5% charcoal-stripped FBS, and either the individual compounds (test group), dimethyl sulfoxide (negative control group), or benzafibrate (positive control group). The cells were further cultured for 20 h. The cells were then washed with PBS and harvested with 200 μL of 1× passive lysis buffer. The intensity of emitted luminescence was determined using a LB 953 Autolumat (EG&G Berthold, Bad Wildbad, Germany). The luminescence intensity ratio (test group/control group) was determined for each compound, and PPARs transcriptional activity was expressed as the relative luminescence intensity of the test compound to that of the control sample.