Regulation and characterization of the dadRAX Locus for D-Amino acid catabolism in Pseudomonas aeruginosa PAO1

Journal of Bacteriology

Volumn 193, Issue 9, 2011, Pages 2107-2115

  He, Weiqing ^a,b   Li, Congran ^a,b   Lu, Chung Dar ^a,c  

^a GEORGIA STATE UNIVERSITY   (United States)

^b INSTITUTE OF MEDICINAL BIOTECHNOLOGY   (China)

^c CHINA MEDICAL UNIVERSITY   (Taiwan)

Author keywords

[No Author keywords available]

Indexed keywords

ALANINE; AMINO ACID; AMINO ACID DERIVATIVE; AMINO ACID RACEMASE DADX; BACTERIAL PROTEIN; BACTERIAL PROTEIN DADRAX; DEXTRO ALANINE; DEXTRO AMINO ACID; DEXTRO AMINO ACID DEHYDROGENASE DADA; DEXTRO GLUTAMIC ACID; DEXTRO GLUTAMINE; DNA; NITROGEN; NUCLEOPROTEIN; OXIDOREDUCTASE; PROTEIN DADR; RACEMASE; RECOMBINANT PROTEIN; RECOMBINANT PROTEIN DADA; TRYPTOPHAN; UNCLASSIFIED DRUG;

AMINO ACID METABOLISM; ANIMAL CELL; ANIMAL EXPERIMENT; ARTICLE; BACTERIAL GROWTH; BACTERIAL STRAIN; BINDING AFFINITY; COMPLEX FORMATION; CONTROLLED STUDY; DNA PROTEIN COMPLEX; ENZYME ACTIVITY; ENZYME SPECIFICITY; GEL MOBILITY SHIFT ASSAY; GENE EXPRESSION; GENE LOCUS; IN VITRO STUDY; IN VIVO STUDY; MUTANT; NONHUMAN; OPERON; PHENOTYPE; PRIORITY JOURNAL; PROMOTER REGION; PSEUDOMONAS AERUGINOSA; SIGNAL TRANSDUCTION; TRANSCRIPTION REGULATION;

AMINO ACIDS; BACTERIAL PROTEINS; BINDING SITES; D-AMINO-ACID OXIDASE; GENE EXPRESSION REGULATION, BACTERIAL; GENE EXPRESSION REGULATION, ENZYMOLOGIC; NITROGEN; PROMOTER REGIONS, GENETIC; PROTEIN BINDING; PSEUDOMONAS AERUGINOSA; SUBSTRATE SPECIFICITY;

BACTERIA (MICROORGANISMS); PSEUDOMONAS AERUGINOSA;

EID: 79955534614     PISSN: 00219193     EISSN: 10985530     Source Type: Journal    
DOI: 10.1128/JB.00036-11     Document Type: Article

References (24)

1. Boulette, M. L., et al. 2009. Characterization of alanine catabolism in Pseudomonas aeruginosa and its importance for proliferation in vivo. J. Bacteriol. 191:6329-6334.
2. Bradford, M. M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72:248-254.
3. Brinkman, A. B., T. J. Ettema, W. M. de Vos, and J. van der Oost. 2003. The Lrp family of transcriptional regulators. Mol. Microbiol. 48:287-294.
4. Challis, G. L., and J. H. Naismith. 2004. Structural aspects of non-ribosomal peptide biosynthesis. Curr. Opin. Struct. Biol. 14:748-756.
5. Chou, H. T., M. Hegazy, and C. D. Lu. 2010. L-Lysine catabolism is controlled by L-arginine and ArgR in Pseudomonas aeruginosa PAO1. J. Bacteriol. 192:5874-5880.
6. Chou, H. T., D. H. Kwon, M. Hegazy, and C. D. Lu. 2008. Transcriptome analysis of agmatine and putrescine catabolism in Pseudomonas aeruginosa PAO1. J. Bacteriol. 190:1966-1975.
7. de los Rios, S., and J. J. Perona. 2007. Structure of the Escherichia coli leucine-responsive regulatory protein Lrp reveals a novel octameric assembly. J. Mol. Biol. 366:1589-1602.
8. Fu, G., et al. 2010. Conformational changes and substrate recognition in Pseudomonas aeruginosa D-arginine dehydrogenase. Biochemistry 49:8535-8545.
9. Goytia, M., et al. 2007. Molecular and structural discrimination of proline racemase and hydroxyproline-2-epimerase from nosocomial and bacterial pathogens. PLoS One 2:e885.
10. Haas, D., B. W. Holloway, A. Schambock, and T. Leisinger. 1977. The genetic organization of arginine biosynthesis in Pseudomonas aeruginosa. Mol. Gen. Genet. 154:7-22.
11. Jilek, A., et al. 2005. Biosynthesis of a D-amino acid in peptide linkage by an enzyme from frog skin secretions. Proc. Natl. Acad. Sci. U. S. A. 102:4235-4239.
12. Kolodkin-Gal, I., et al. 2010. D-Amino acids trigger biofilm disassembly. Science 328:627-629.
13. Kurnasov, O., et al. 2003. Aerobic tryptophan degradation pathway in bacteria: novel kynurenine formamidase. FEMS Microbiol. Lett. 227:219-227.
14. Lam, H., et al. 2009. D-amino acids govern stationary phase cell wall remodeling in bacteria. Science 325:1552-1555.
15. Li, C., and C. D. Lu. 2009. Arginine racemization by coupled catabolic and anabolic dehydrogenases. Proc. Natl. Acad. Sci. U. S. A. 106:906-911.
16. Li, C., X. Yao, and C. D. Lu. 2010. Regulation of the dauBAR operon and characterization of D-amino acid dehydrogenase DauA in arginine and lysine catabolism of Pseudomonas aeruginosa PAO1. Microbiology 156: 60-71.
17. Lobocka, M., J. Hennig, J. Wild, and T. Klopotowski. 1994. Organization and expression of the Escherichia coli K-12 dad operon encoding the smaller subunit of D-amino acid dehydrogenase and the catabolic alanine racemase. J. Bacteriol. 176:1500-1510.
18. Olsiewski, P. J., G. J. Kaczorowski, and C. Walsh. 1980. Purification and properties of D-amino acid dehydrogenase, an inducible membrane-bound iron-sulfur flavoenzyme from Escherichia coli B. J. Biol. Chem. 255:4487-4494.
19. Strych, U., H. C. Huang, K. L. Krause, and M. J. Benedik. 2000. Characterization of the alanine racemases from Pseudomonas aeruginosa PAO1. Curr. Microbiol. 41:290-294.
20. Takahashi, E., M. Furui, H. Seko, and T. Shibatani. 1997. D-Lysine production from L-lysine by successive chemical racemization and microbial asymmetric degradation. Appl. Microbiol. Biotechnol. 47:347-351.
21. Wasserman, S. A., C. T. Walsh, and D. Botstein. 1983. Two alanine racemase genes in Salmonella typhimurium that differ in structure and function. J. Bacteriol. 153:1439-1450.
22. Yang, Z., and C. D. Lu. 2007. Functional genomics enables identification of genes of the arginine transaminase pathway in Pseudomonas aeruginosa. J. Bacteriol. 189:3945-3953.
23. Zhi, J., E. Mathew, and M. Freundlich. 1998. In vitro and in vivo characterization of three major dadAX promoters in Escherichia coli that are regulated by cyclic AMP-CRP and Lrp. Mol. Gen. Genet. 258:442-447.
24. Zhi, J., E. Mathew, and M. Freundlich. 1999. Lrp binds to two regions in the dadAX promoter region of Escherichia coli to repress and activate transcription directly. Mol. Microbiol. 32:29-40.