Evaluation of four different diagnostic tests to detect Clostridium difficile in piglets

Journal of Clinical Microbiology

Volumn 49, Issue 5, 2011, Pages 1816-1821

  Keessen, E C ^a   Hopman, N E M ^a   Van Leengoed, L A M G ^b   Van Asten, A J A M ^b   Hermanus, C ^c   Kuijper, E J ^c   Lipman, L J A ^a  

^a UTRECHT UNIVERSITY   (Netherlands)

^b UTRECHT UNIVERSITY   (Netherlands)

^c LEIDEN UNIVERSITY MEDICAL CENTER   (Netherlands)

Author keywords

[No Author keywords available]

Indexed keywords

ALGORITHM; ARTICLE; BACTERIUM CULTURE; BACTERIUM DETECTION; CELL ASSAY; CLOSTRIDIUM DIFFICILE; CLOSTRIDIUM DIFFICILE INFECTION; CONTROLLED STUDY; CYTOTOXICITY TEST; DIAGNOSTIC TEST ACCURACY STUDY; ENZYME IMMUNOASSAY; FECES ANALYSIS; HERD; INFECTIOUS DIARRHEA; INTERMETHOD COMPARISON; NONHUMAN; PREDICTION; PRIORITY JOURNAL; REAL TIME POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; SWINE DISEASE;

ANIMALS; CLOSTRIDIUM DIFFICILE; CLOSTRIDIUM INFECTIONS; DIAGNOSTIC TESTS, ROUTINE; ENZYME-LINKED IMMUNOSORBENT ASSAY; POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; SWINE; SWINE DISEASES;

ANIMALIA; BACTERIA (MICROORGANISMS); CLOSTRIDIUM DIFFICILE; NUCLEOPOLYHEDROVIRUS; SUIDAE;

EID: 79955528101     PISSN: 00951137     EISSN: 1098660X     Source Type: Journal    
DOI: 10.1128/JCM.00242-11     Document Type: Article

References (30)

1. Alvarez-Perez, S., P. Alba, J. L. Blanco, and M. E. Garcia. 2009. Detection of toxigenic Clostridium difficile in pig faeces by PCR. Veterinarni Medicina 54:360-366.
2. Anderson, M. A., and J. G. Songer. 2008. Evaluation of two enzyme immunoassays for detection of Clostridium difficile toxins A and B in swine. Vet. Microbiol. 128:204-206.
3. Arroyo, L. G., H. Staempfli, and J. S. Weese. 2007. Molecular analysis of Clostridium difficile isolates recovered from horses with diarrhea. Vet. Microbiol. 120:179-183.
4. Bidet, P., et al. 2000. Comparison of PCR-ribotyping, arbitrarily primed PCR, and pulsed-field gel electrophoresis for typing Clostridium difficile. J. Clin. Microbiol. 38:2484-2487.
5. Chouicha, N., and S. L. Marks. 2006. Evaluation of five enzyme immunoassays compared with the cytotoxicity assay for diagnosis of Clostridium difficile-associated diarrhea in dogs. J. Vet. Diagn. Invest. 18:182-188.
6. Cohen, S. H., et al. 2010. Clinical practice guidelines for Clostridium difficile infection in adults: 2010 update by the society for health care epidemiology of America (SHEA) and the infectious diseases society of America (IDSA). Infect. Control Hosp. Epidemiol. 31:431-455.
7. Crobach, M. J. T., O. M. Dekkers, M. H. Wilcox, and E. J. Kuijper. 2009. European Society of Clinical Microbiology and Infectious Diseases (ESCMID): data review and recommendations for diagnosing Clostridium difficile-infection (CDI). Clin. Microbiol. Infect. 15:1053-1066.
8. Debast, S. B., et al. 2009. Clostridium difficile PCR ribotype 078 toxinotype V found in diarrhoeal pigs identical to isolates from affected humans. Environ. Microbiol. 11:505-511.
9. Delmee, M., J. Van Broeck, A. Simon, M. Janssens, and V. Avesani. 2005. Laboratory diagnosis of Clostridium difficile-associated diarrhoea: a plea for culture. J. Med. Microbiol. 54:187-191.
10. Freeman, J., and M. H. Wilcox. 2003. The effects of storage conditions on viability of Clostridium difficile vegetative cells and spores and toxin activity in human faeces. J. Clin. Pathol. 56:126-128.
11. Goorhuis, A., et al. Clostridium difficile PCR ribotype 078: an emerging strain in humans and in pigs? J. Clin. Microbiol. 46:1157-1158.
12. Keessen, E. C., et al. 2010. Prevalence of Clostridium difficile in swine thought to have Clostridium difficile infections (CDI) in eleven swine operations in the Netherlands. Tijdschr. Diergeneeskd. 135:134-137.
13. Kuijper, E. J., R. J. van den Berg, and S. Debast. 2006. Clostridium difficile ribotype 027, toxinotype III, the Netherlands. Emerg. Infect. Dis. 12:827-828.
14. Lozniewski, A., C. Rabaud, E. Dotto, M. Weber, and F. Mory. 2001. Laboratory diagnosis of Clostridium difficile-associated diarrhea and colitis: usefulness of Premier Cytoclone A+B enzyme immunoassay for combined detection of stool toxins and toxigenic C. difficile strains. J. Clin. Microbiol. 39:1996-1998.
15. Lyerly, D. M., N. M. Sullivan, and T. D. Wilkins. 1983. Enzyme-linked immunosorbent assay for Clostridium difficile toxin A. J. Clin. Microbiol. 17:72-78.
16. Magdesian, K. G., D. C. Hirsh, S. S. Jang, L. M. Hansen, and J. E. Madigan. 2002. Characterization of Clostridium difficile isolates from foals with diarrhea: 28 cases (1993-1997). J. Am. Vet. Med. Assoc. 220:67-73.
17. Norman, K. N., et al. 2009. Varied prevalence of Clostridium difficile in an integrated swine operation. Anaerobe 15:256-260.
18. Paltansing, S., et al. 2007. Characteristics and incidence of Clostridium difficile-associated disease in The Netherlands, 2005. Clin. Microbiol. Infect. 13:1058-1064.
19. Post, K. W., B. H. Jost, and J. G. Songer. 2002. Evaluation of a test for Clostridium difficile toxins A and B for the diagnosis of neonatal swine enteritis. J. Vet. Diagn. Invest. 14:258-259.
20. Reller, M. E., et al. 2007. Yield of stool culture with isolate toxin testing versus a two-step algorithm including stool toxin testing for detection of toxigenic Clostridium difficile. J. Clin. Microbiol. 45:3601-3605.
21. Songer, J. G. 2004. The emergence of Clostridium difficile as a pathogen of food animals. Anim. Health Res. Rev. 5:321-326.
22. Songer, J. G., and M. A. Anderson. 2006. Clostridium difficile: an important pathogen of food animals. Anaerobe 12:1-4.
23. Tenover, F. C., et al. 2010. Impact of strain type on detection of toxigenic Clostridium difficile: comparison of molecular diagnostic and enzyme immunoassay approaches. J. Clin. Microbiol. 48:3719-3724.
24. Thonnard, J., F. Carreer, V. Avesani, and M. Delmee. 1996. Toxin A detection on Clostridium difficile colonies from 24-h cultures. Clin. Microbiol. Infect. 2:50-54.
25. van den Berg, R. J., et al. 2005. Prospective multicenter evaluation of a new immunoassay and real-time PCR for rapid diagnosis of Clostridium difficile-associated diarrhea in hospitalized patients. J. Clin. Microbiol. 43:5338-5340.
26. van den Berg, R. J., et al. 2007. Evaluation of real-time PCR and conventional diagnostic methods for the detection of Clostridium difficileassociated diarrhoea in a prospective multicentre study. J. Med. Microbiol. 56:36-42.
27. van Leengoed, L., S. B. Debast, A. A. Bergwerff, and E. J. Kuiper. 2008. Neonatal diarrhea in piglets caused by Clostridium difficile. Proc. 20th IPVF Cong., p. 134.
28. Weese, J. S., H. R. Staempfli, and J. F. Prescott. 2000. Survival of Clostridium difficile and its toxins in equine feces: implications for diagnostic test selection and interpretation. J. Vet. Diagn. Invest. 12:332-336.
29. Yaeger, M., N. Funk, and L. Hoffman. 2002. A survey of agents associated with neonatal diarrhea in Iowa swine including Clostridium difficile and porcine reproductive and respiratory syndrome virus. J. Vet. Diagn. Invest. 14:281-287.
30. Yaeger, M. J., J. M. Kinyon, and J. Glenn Songer. 2007. A prospective, case control study evaluating the association between Clostridium difficile toxins in the colon of neonatal swine and gross and microscopic lesions. J. Vet. Diagn. Invest. 19:52-59.