High-resolution melting curve analysis to establish CYP2C19*2 single nucleotide polymorphism: Comparison with hydrolysis SNP analysis

Molecular and Cellular Probes

Volumn 25, Issue 2-3, 2011, Pages 130-133

  Temesvári, Manna ^a   Paulik, József ^b   Kóbori, László ^c   Monostory, Katalin ^a  

^a CHEMICAL RESEARCH CENTER   (Hungary)

^b Bio Rad Laboratories Hungary Ltd   (Hungary)

^c SEMMELWEIS UNIVERSITY   (Hungary)

Author keywords

CYP2C19*2; High resolution melting curve analysis; Hydrolysis SNP analysis; Single nucleotide polymorphism

Indexed keywords

CYTOCHROME P450 2C19;

ARTICLE; ASSAY; CONTROLLED STUDY; COST EFFECTIVENESS ANALYSIS; FEMALE; GENOTYPE; HIGH RESOLUTION MELTING CURVE ANALYSIS; HUMAN; HYDROLYSIS; MAJOR CLINICAL STUDY; MALE; PRIORITY JOURNAL; SINGLE NUCLEOTIDE POLYMORPHISM;

ARYL HYDROCARBON HYDROXYLASES; DNA PRIMERS; GENOTYPE; HUMANS; HYDROLYSIS; LIVER TRANSPLANTATION; MICROSOMES, LIVER; NUCLEIC ACID DENATURATION; POLYMERASE CHAIN REACTION; POLYMORPHISM, SINGLE NUCLEOTIDE; REPRODUCIBILITY OF RESULTS; TISSUE DONORS;

EID: 79955483490     PISSN: 08908508     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.mcp.2011.01.003     Document Type: Article

References (17)

1. Solus J.F., Arietta B.J., Harris J.R., Sexton D.P., Steward J.Q., McMunn C., et al. Genetic variation in eleven phase I drug metabolism genes in an ethnically diverse population. Pharmacogenomics 2004, 5:895-931.
2. Tamasi V., Vereczkey L., Falus A., Monostory K. Some aspects of interindividual variations in the metabolism of xenobiotics. Inflamm Res 2003, 52:322-333.
3. Guengerich F.P. Cytochrome P450s and other enzymes in drug metabolism and toxicity. AAPS J 2006, 8:E101-E111.
4. Ingelman-Sundberg M. Pharmacogenetics: an opportunity for a safer and more efficient pharmacotherapy. J Intern Med 2001, 250:186-200.
5. Brockmöller J., Kirchheiner J., Meisel C., Roots I. Pharmacogenetic diagnostics of cytochrome P450 polymorphisms in clinical drug development and in drug treatment. Pharmacogenomics 2000, 1:125-151.
6. Goldstein J.A. Clinical relevance of genetic polymorphisms in the human CYP2C subfamily. Br J Clin Pharmacol 2001, 52:349-355.
7. Ingelman-Sundberg M. Genetic polymorphisms of cytochrome P450 2D6 (CYP2D6): clinical consequences, evolutionary aspects and functional diversity. Pharmacogenomics J 2005, 5:6-13.
8. Srivastava P.K., Yun C.-H., Beaune P.H., Ged C., Guengerich F.P. Separation of human liver microsomal tolbutamide hydroxylase and (S)-mephenytoin 4'-hydroxylase cytochrome P-450 enzymes. Mol Pharmacol 1991, 40:69-79.
9. Rendic S., Di Carlo F.J. Human cytochrome P450 enzymes: a status report summarizing their reactions, substrates, inducers, and inhibitors. Drug Metab Rev 1997, 29:413-580.
10. Desta Z., Zhao X., Shin J.G., Flockhart D.A. Clinical significance of the cytochrome P450 2C19 genetic polymorphism. Clin Pharmacokinet 2002, 41:913-958.
11. Helsby N.A. Pheno- or genotype for the CYP2C19 drug metabolism polymorphism: the influence of disease. Proc West Pharmacol Soc 2008, 51:5-10.
12. Wittwer T.C., Reed G.H., Gundry C.N., Vandersteen J.G., Pryor R.J. High-resolution genotyping by amplicon melting analysis using LC Green. Clin Chem 2003, 49:853-860.
13. Mao F., Leung W.-Y., Xin X. Characterization of EvaGreen and the implication of its physicochemical properties for qPCR applications. BMC Biotechnol 2007, 7:76.
14. van der Hoeven T., Coon M.J. Preparation and properties of partially purified cytochrome P450 and reduced nicotinamide adenine dinucleotide phosphate-cytochrome P450 reductase from rabbit liver microsomes. J Biol Chem 1974, 249:6302-6310.
15. de Morais S.M.F., Wilkinson G.R., Blaisdell J., Nakamura K., Meyer U.A., Goldstein J.A. The major genetic defect responsible for the polymorphism of S-mephenytoin metabolism in humans. J Biol Chem 1994, 269:15419-15422.
16. Tamminga W.J., Wemer J., Oosterhuis B., de Zeeuw R.A., de Leij L.F.M.H., Jonkman J.H.G. The prevalence of CYP2D6 and CYP2C19 genotypes in a population of healthy Dutch volunteers. Eur J Clin Pharmacol 2001, 57:717-722.
17. Scordo M.G., Caputi A.P., D'Arrigo C., Fava G., Spina E. Allele and genotype frequencies of CYP2C9, CYP2C19 and CYP2D6 in an Italian population. Pharmacol Res 2004, 50:195-200.