Bacterial screening by flow cytometry offers potential for extension of platelet storage: Results of 14 months of active surveillance

Transfusion Medicine

Volumn 21, Issue 3, 2011, Pages 175-182

  Vollmer, T ^a   Engemann, J ^a   Kleesiek, K ^a   Dreier, J ^a  

^a RUHR UNIVERSITY BOCHUM   (Germany)

Author keywords

Flow cytometry; Platelets shelf life; Point of issue testing; Rapid method; Semi automation; Sterility testing

Indexed keywords

ESTERASE; THROMBOCYTE CONCENTRATE;

ALGORITHM; APHERESIS; ARTICLE; BACTERIUM CONTAMINATION; BLOOD BANK; CONTROLLED STUDY; ENZYME ACTIVITY; FLOW CYTOMETRY; HUMAN; HUMAN CELL; NONHUMAN; PROPIONIBACTERIUM; SCREENING; SENSITIVITY ANALYSIS; STAPHYLOCOCCUS AUREUS; THROMBOCYTE PRESERVATION; THROMBOCYTE TRANSFUSION;

BACTERIA; BLOOD PLATELETS; BLOOD PRESERVATION; FLOW CYTOMETRY; HUMANS; QUALITY CONTROL; TIME FACTORS;

EID: 79955096890     PISSN: 09587578     EISSN: 13653148     Source Type: Journal    
DOI: 10.1111/j.1365-3148.2011.01070.x     Document Type: Article

References (37)

1. Arbeitskreis Blut. (2008) Votum 38: Festlegung der Haltbarkeitsfrist von Thrombozytenkonzentraten mit dem Ziel der Reduktion lebensbedrohlicher septischer Transfusionsreaktionen durch bakterielle Kontamination. Bundesgesundheitsblatt, 51, 1484.
2. AuBuchon, J.P. (2006) The reliability of bacterial detection in platelets. ISBT Science Series, 1, 59-63.
3. Blajchman, M.A., Goldman, M. & Baeza, F. (2004) Improving the bacteriological safety of platelet transfusions. Transfusion Medicine Reviews, 18, 11-24.
4. Brecher, M.E. & Hay, S.N. (2005) Bacterial contamination of blood components. Clinical Microbiology Reviews, 18, 195-204.
5. Brecher, M. (2002) Methods to minimize transfusion related bacterial sepsis. In: Advances in Transfusion Safety (eds Brown, F. & Seitz, E.), 69-84. Karger, Basel.
6. Castro, E., Bueno, J.L., Barea, L. & Gonzalez, R. (2005) Feasibility of implementing an automated culture system for bacteria screening in platelets in the blood bank routine. Transfusion Medicine, 15, 185-195.
7. Dreier, J., Störmer, M., Pichl, L., Schottstedt, V., Grolle, A., Bux, J. & Kleesiek, K. (2008) Sterility screening of platelet concentrates: questioning the optimal test strategy. Vox Sanguinis, 95, 181-188.
8. Dreier, J., Vollmer, T. & Kleesiek, K. (2009) Novel flow cytometry-based screening for bacterial contamination of donor platelet preparations compared with other rapid screening methods. Clinical Chemistry, 55, 1492-1502.
9. Eder, A.F., Kennedy, J.M., Dy, B.A. et al. (2007) Bacterial screening of apheresis platelets and the residual risk of septic transfusion reactions: the American Red Cross experience (2004-2006). Transfusion, 47, 1134-1142.
10. Fang, C.T., Chambers, L.A., Kennedy, J. et al. (2005) Detection of bacterial contamination in apheresis platelet products: American Red Cross experience, 2004. Transfusion, 45, 1845-1852.
11. Günthard, H., Hany, A., Turina, M. & Wust, J. (1994) Propionibacterium acnes as a cause of aggressive aortic valve endocarditis and importance of tissue grinding: case report and review. Journal of Clinical Microbiology, 32, 3043-3045.
12. Hundhausen, T. & Muller, T.H. (2005) False-positive alarms for bacterial screening of platelet concentrates with BacT/ALERT new-generation plastic bottles: a multicenter pilot study. Transfusion, 45, 1267-1274.
13. Karo, O., Wahl, A., Nicol, S.B. et al. (2008) Bacteria detection by flow cytometry. Clinical Chemistry and Laboratory Medicine, 46, 947-953.
14. Larsen, C.P., Ezligini, F., Hermansen, N.O. & Kjeldsen-Kragh, J. (2005) Six years' experience of using the BacT/ALERT system to screen all platelet concentrates, and additional testing of outdated platelet concentrates to estimate the frequency of false-negative results. Vox Sanguinis, 88, 93-97.
15. McDonald, C.P. (2006) Bacterial risk reduction by improved donor arm disinfection, diversion and bacterial screening. Transfusion Medicine, 16, 381-396.
16. Mohammadi, T., Savelkoul, P.H., Pietersz, R.N. & Reesink, H.W. (2006) Applications of real-time PCR in the screening of platelet concentrates for bacterial contamination. Expert Review of Molecular Diagnosis, 6, 865-872.
17. Mohr, H., Bayer, A., Gravemann, U. & Muller, T.H. (2006a) Elimination and multiplication of bacteria during preparation and storage of buffy coat-derived platelet concentrates. Transfusion, 46, 949-955.
18. Mohr, H., Lambrecht, B., Bayer, A., Spengler, H.P., Nicol, S.B., Montag, T. & Muller, T.H. (2006b) Basics of flow cytometry-based sterility testing of platelet concentrates. Transfusion, 46, 41-49.
19. Montag, T. (2006) Perspectives and limitations in the bacterial screening of platelet concentrates. Journal of Laboratory Medicine, 30, 60-65.
20. Montag, T. (2008) Strategies of bacteria screening in cellular blood components. Clinical Chemistry and Laboratory Medicine, 46, 926-932.
21. Munksgaard, L., Albjerg, L., Lillevang, S.T., Gahrn-Hansen, B. & Georgsen, J. (2004) Detection of bacterial contamination of platelet components: six years' experience with the BacT/ALERT system. Transfusion, 44, 1166-1173.
22. Murphy, W.G., Foley, M., Doherty, C., Tierney, G., Kinsella, A., Salami, A., Cadden, E. & Coakley, P. (2008) Screening platelet concentrates for bacterial contamination: low numbers of bacteria and slow growth in contaminated units mandate an alternative approach to product safety. Vox Sanguinis, 95, 13-19.
23. Palavecino, E.L., Yomtovian, R.A. & Jacobs, M.R. (2006) Detecting bacterial contamination in platelet products. Clinica y Laboratorio, 52, 443-456.
24. Palavecino, E.L., Yomtovian, R.A. & Jacobs, M.R. (2010) Bacterial contamination of platelets. Transfusion and Apheresis Science, 42, 71-82.
25. Pink, J.M., MacCallum, S., Ribeiro, A. & Wylie, B.R. (1993) Platelet transfusion-related sepsis. Australian and New Zealand Journal of Medicine, 23, 717.
26. Prowse, C. (2007) Zero tolerance. Transfusion, 47, 1106-1109.
27. Ramirez-Arcos, S., Jenkins, C., Dion, J., Bernier, F., Delage, G. & Goldman, M. (2007) Canadian experience with detection of bacterial contamination in apheresis platelets. Transfusion, 47, 421-429.
28. Rood, I.G., Koppelman, M.H., Pettersson, A. & Savelkoul, P.H. (2008) Development of an internally controlled PCR assay for broad range detection of bacteria in platelet concentrates. Journal of Microbiological Methods, 75, 64-69.
29. Schmidt, M., Hourfar, M.K., Nicol, S.B., Spengler, H.P., Montag, T. & Seifried, E. (2006) FACS technology used in a new rapid bacterial detection method. Transfusion Medicine, 16, 355-361.
30. Schmidt, M., Karakassopoulos, A., Burkhart, J. et al. (2007) Comparison of three bacterial detection methods under routine conditions. Vox Sanguinis, 92, 15-21.
31. Schneider, T., Breviere, D., Taillefer, M.F., Pujol-Rey, A. & Huart, J.J. (2000) Bacterial contamination of platelet concentrates by Propionibacterium acnes. Transfusion Clinique et Biologique, 7, 540-546.
32. Schrezenmeier, H., Walther-Wenke, G., Muller, T.H. et al. (2007) Bacterial contamination of platelet concentrates: results of a prospective multicenter study comparing pooled whole blood-derived platelets and apheresis platelets. Transfusion, 47, 644-652.
33. Störmer, M., Kleesiek, K. & Dreier, J. (2008) Propionibacterium acnes lacks the capability to proliferate in platelet concentrates. Vox Sanguinis, 94, 193-201.
34. Wagner, S.J., Friedman, L.I. & Dodd, R.Y. (1994) Transfusion-associated bacterial sepsis. Clinical Microbiology Reviews, 7, 290-302.
35. Walther-Wenke, G. (2006) Bacterial contamination of blood components-incidence and significance for homologous and autologous transfusion. Journal of Laboratory Medicine, 30, 66-73.
36. de Korte, D., Curvers, J., de Kort, W.L., Hoekstra, T., van der Poel, C.L., Beckers, E.A. & Marcelis, J.H. (2006) Effects of skin disinfection method, deviation bag, and bacterial screening on clinical safety of platelet transfusions in the Netherlands. Transfusion, 46, 476-485.
37. te Boekhorst, P.A., Beckers, E.A., Vos, M.C., Vermeij, H. & van Rhenen, D.J. (2005) Clinical significance of bacteriologic screening in platelet concentrates. Transfusion, 45, 514-519.