Conditions optimized for the preparation of single-stranded DNA (ssDNA) employing lambda exonuclease digestion in generating DNA aptamer

World Journal of Microbiology and Biotechnology

Volumn 27, Issue 5, 2011, Pages 1167-1173

  Citartan, Marimuthu ^a   Tang, Thean Hock ^a   Tan, Soo Choon ^b   Gopinath, Subash C B ^c  

^a ADVANCED MEDICAL AND DENTAL INSTITUTE   (Malaysia)

^b INSTITUTE FOR RESEARCH IN MOLECULAR MEDICINE INFORMM   (Malaysia)

^c NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGY AIST   (Japan)

Author keywords

Aptamer; Lambda exonuclease; Optimization; Single stranded DNA

Indexed keywords

APTAMERS; EXONUCLEASE; LAMBDA EXONUCLEASE; NUCLEASE DIGESTION; OPTIMIZED CONDITIONS; PCR PRODUCTS; POLYACRYLAMIDE GELS; SEPARATION TECHNIQUES; SINGLE STRANDED DNA; SINGLE-STRANDED DNA (SS-DNA); STRAND SEPARATION; STREPTAVIDIN; SYSTEMATIC EVOLUTION OF LIGANDS BY EXPONENTIAL ENRICHMENTS; TIME COURSE;

DNA; NUCLEIC ACIDS; OPTIMIZATION; UREA;

POLYMERASE CHAIN REACTION;

EID: 79955069333     PISSN: 09593993     EISSN: None     Source Type: Journal    
DOI: 10.1007/s11274-010-0563-8     Document Type: Article

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