In vitro selection of single-stranded DNA aptamers that bind human pro-urokinase

Nucleosides, Nucleotides and Nucleic Acids

Volumn 23, Issue 6-7, 2004, Pages 891-893

  Skrypina, Natalia A ^a   Savochkina, Larissa P ^a   Beabealashvilli, Robert Sh ^a  

^a Russian Research and Practical Centre of Cardiology   (Russian Federation)

Author keywords

Aptamer; Pro urokinase; SELEX

Indexed keywords

APTAMER; PLASMIN; PROUROKINASE; SINGLE STRANDED DNA;

CONFERENCE PAPER; DNA ISOLATION; GENE EXPRESSION SYSTEM; ISOTOPE LABELING; POLYACRYLAMIDE GEL ELECTROPHORESIS; POLYMERASE CHAIN REACTION; PROTEIN DNA BINDING; SEQUENCE ANALYSIS; SEQUENCE HOMOLOGY;

BASE SEQUENCE; DNA, SINGLE-STRANDED; HUMANS; MOLECULAR SEQUENCE DATA; NUCLEIC ACID CONFORMATION; PROTEIN BINDING; RECOMBINANT PROTEINS; SEQUENCE HOMOLOGY, NUCLEIC ACID; URINARY PLASMINOGEN ACTIVATOR;

EID: 7644237172     PISSN: 15257770     EISSN: None     Source Type: Journal    
DOI: 10.1081/NCN-200026037     Document Type: Conference Paper

References (6)

1. Bock, L.C.; Griffin, L.C.; Latham, J.A.; Vermaas, E.H.; Toole, J.J. Selection of single stranded DNA molecules that bind and inhibit human thrombin. Nature 1992, 355, 564-566.
2. Breaker, R.R. DNA aptamers and DNA enzymes. Curr. Opin. Chem. Biol. 1997, 1, 26-31.
3. Ellington, A.D.; Szostak, J.W. Selection in vitro of single-stranded DNA molecules that fold into specific ligand-binding structures. Nature 1992, 355, 850-852.
4. Blank, M.; Weinschenk, T.; Priemer, M.; Schluesener, H. Systematic evolution of a DNA aptamer binding to rat brain tumor microvessels. Selective targeting of endothelial regulatory protein pigpen. J. Biol. Chem. 2001, 276, 16464-16468.
5. Blackburn, P.; Wilson, G.; Moore, S. Ribonuclease inhibitor from human placenta. J. Biol. Chem. 1977, 252, 5904-5910.
6. Meyerhans, A.; Vartanian, J.P.; Wain-Hobson, S. Strand specific PCR amplification of low copy number DNA. Nucleic Acids Res. 1992, 20, 521-523.