A new animal model for implant-related infected non-unions after intramedullary fixation of the tibia in rats with fluorescent in situ hybridization of bacteria in bone infection

Bone

Volumn 48, Issue 5, 2011, Pages 1146-1153

  Alt, Volker ^a,b   Lips, Katrin S ^b   Henkenbehrens, Christoph ^a,b   Muhrer, Dominik ^a,b   Cavalcanti Garcia, Marcia ^b   Sommer, Ursula ^b   Thormann, Ulrich ^a,b   Szalay, Gabor ^a,b   Heiss, Christian ^a,b   Pavlidis, Theodoros ^a,b   Domann, Eugen ^a   Schnettler, Reinhard ^a,b  

^a UNIVERSITY HOSPITAL GIESSEN   (Germany)

^b JUSTUS LIEBIG UNIVERSITY   (Germany)

Author keywords

Animal model; Biofilm; FISH; Infection; Non union

Indexed keywords

CEFTRIAXONE;

ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ANTIBIOTIC PROPHYLAXIS; ARTICLE; BACTERIUM CONTAMINATION; BACTERIUM DETECTION; BACTERIUM IDENTIFICATION; BIOFILM; BONE INFECTION; BONE RADIOGRAPHY; CONTROLLED STUDY; DEVICE INFECTION; DISEASE MODEL; FLUORESCENCE IN SITU HYBRIDIZATION; FRACTURE HEALING; FRACTURE NONUNION; INTRAMEDULLARY NAILING; MICRO-COMPUTED TOMOGRAPHY; NONHUMAN; RAT; SCANNING ELECTRON MICROSCOPY; STAPHYLOCOCCUS AUREUS; TIBIA OSTEOTOMY;

ANIMALS; BIOFILMS; BONE DISEASES, INFECTIOUS; DISEASE MODELS, ANIMAL; FRACTURE FIXATION, INTRAMEDULLARY; FRACTURES, UNUNITED; IN SITU HYBRIDIZATION, FLUORESCENCE; PROSTHESIS-RELATED INFECTIONS; RATS; RATS, SPRAGUE-DAWLEY; STAPHYLOCOCCUS AUREUS; TIBIAL FRACTURES; X-RAY MICROTOMOGRAPHY; X-RAYS;

EID: 79954625127     PISSN: 87563282     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.bone.2011.01.018     Document Type: Article

References (23)

1. Court-Brown C.M. Reamed intramedullary tibial nailing: an overview and analysis of 1106 cases. J Orthop Trauma 2004, 18:96-101.
2. Malik M.H., Harwood P., Diggle P., Khan S.A. Factors affecting rates of infection and nonunion in intramedullary nailing. J Bone Joint Surg Br 2004, 86:556-560.
3. Cierny G., DiPasquale D. Treatment of chronic infection. J Am Acad Orthop Surg 2006, 14:S105-S110.
4. An Y.H., Kang Q.K., Arciola C.R. Animal models of osteomyelitis. Int J Artif Organs 2006, 29:407-420.
5. Lucke M., Schmidmaier G., Sadoni S., Wildemann B., Schiller R., Stemberger A., et al. A new model of implant-related osteomyelitis in rats. J Biomed Mater Res B Appl Biomater 2003, 67:593-602.
6. Lucke M., Wildemann B., Sadoni S., Surke C., Schiller R., Stemberger A., et al. Systemic versus local application of gentamicin in prophylaxis of implant-related osteomyelitis in a rat model. Bone 2005, 36:770-778.
7. Alt V., Bitschnau A., Osterling J., Sewing A., Meyer C., Kraus R., et al. The effects of combined gentamicin-hydroxyapatite coating for cementless joint prostheses on the reduction of infection rates in a rabbit infection prophylaxis model. Biomaterials 2006, 27:4627-4634.
8. Nijhof M.W., Dhert W.J., Fleer A., Vogely H.C., Verbout A.J. Prophylaxis of implant-related staphylococcal infections using tobramycin-containing bone cement. J Biomed Mater Res 2000, 52:754-761.
9. Bonnarens F., Einhorn T.A. Production of a standard closed fracture in laboratory animal bone. J Orthop Res 1984, 2:97-101.
10. Andriole V.T., Nagel D.A., Southwick W.O. Chronic staphylococcal osteomyelitis: an experimental model. Yale J Biol Med 1974, 47:33-39.
11. Trampuz A., Piper K.E., Jacobson M.J., Hanssen A.D., Unni K.K., Osmon D.R., et al. Sonication of removed hip and knee prostheses for diagnosis of infection. N Engl J Med 2007, 357:654-663.
12. Moter A., Gobel U.B. Fluorescence in situ hybridization (FISH) for direct visualization of microorganisms. J Microbiol Meth 2000, 41:85-112.
13. Tunney M.M., Patrick S., Curran M.D., et al. Detection of prosthetic hip infection at revision arthroplasty by immunofluorescent microscopy and PCR amplification of the bacterial 16S rRNA gene. J Clin Microbiol 1999, 37:3281-3290.
14. Bentley R.W., Harland N.M., Leigh J.A., Collins M.D. A Staphylococcus aureus-specific oligonucleotide probe derived from 16S rRNA gene sequences. Lett Appl Microbiol 1993, 16:203-206.
15. Fuchs B.M., Wallner G., Beisker W., Schwippl I., Ludwig W., Amann R. Flow cytometric analysis of the in situ accessibility of Escherichia coli 16S rRNA for fluorescently labeled oligonucleotide probes. Appl Environ Microbiol 1998, 64:4973-4982.
16. Amann R.I., Binder B.J., Olson R.J., Chisholm S.W., Devereux R., Stahl D.A. The domain-specific probe EUB338 is insufficient for the detection of all bacteria: development and evaluation of a more comprehensive probe set. Syst Appl Microbiol 1999, 22:434-444.
17. Govender S., Csimma C., Genant H.K., Valentin-Opran A., Amit Y., Arbel R., et al. Recombinant human bone morphogenetic protein-2 for treatment of open tibial fractures: a prospective, controlled, randomized study of four hundred and fifty patients. J Bone Joint Surg Am 2002, 84-A:2123-2134.
18. Donath K., Breuner G. A method for the study of undecalcified bones and teeth with attached soft tissues. The Sage-Schliff (sawing and grinding) technique. J Oral Pathol 1982, 11:318-326.
19. Chen X., Tsukayama D.T., Kidder L.S., Bourgeault C.A., Schmidt A.H., Lew W.D. Characterization of a chronic infection in an internally-stabilized segmental defect in the rat femur. J Orthop Res 2005, 23:816-823.
20. Friedlaender G.E., Perry C.R., Cole J.D., Cook S.D., Cierny G., Muschler G.F., et al. Osteogenic protein-1 (bone morphogenetic protein-7) in the treatment of tibial nonunions. J Bone Joint Surg Am 2001, 83-A(Suppl 1):S151-S158.
21. Chen X., Schmidt A.H., Tsukayama D.T., Bourgeault C.A., Lew W.D. Recombinant human osteogenic protein-1 induces bone formation in a chronically infected, internally stabilized segmental defect in the rat femur. J Bone Joint Surg Am 2006, 88:1510-1523.
22. Wright J.A., Nair S.P. Interaction of staphylococci with bone. Int J Med Microbiol 2010, 300:193-204.
23. Krimmer V., Merkert H., von E.C., Frosch M., Eulert J., Lohr J.F., et al. Detection of Staphylococcus aureus and Staphylococcus epidermidis in clinical samples by 16S rRNA-directed in situ hybridization. J Clin Microbiol 1999, 37:2667-2673.