Safety and persistence of orally administered human Lactobacillus sp. strains in healthy adults

Beneficial Microbes

Volumn 2, Issue 1, 2011, Pages 79-90

  Hutt P ^a   Koll P ^a   Stsepetova, J ^a   Alvarez, B ^b   Mandar R ^a   Krogh Andersen, K ^b   Marcotte, H ^b   Hammarstrom L ^b   Mikelsaar, M ^a  

^a UNIVERSITY OF TARTU   (Estonia)

^b KAROLINSKA UNIVERSITY HOSPITAL   (Sweden)

Author keywords

Colonisation; Cultivation; Probiotic; Real time PCR; Survival

Indexed keywords

PROBIOTIC AGENT;

ABDOMINAL DISEASE; ABDOMINAL PAIN; ADULT; ARTICLE; BACTERIAL COLONIZATION; BACTERIAL COUNT; BACTERIAL STRAIN; BACTERIAL SURVIVAL; BACTERIUM CULTURE; BACTERIUM IDENTIFICATION; BIOTECHNOLOGY; BLOATING; BLOOD ANALYSIS; COLONY FORMING UNIT; CONTROLLED CLINICAL TRIAL; CONTROLLED STUDY; DENATURING GRADIENT GEL ELECTROPHORESIS; DOSE RESPONSE; DRUG DETERMINATION; DRUG MEGADOSE; DRUG SAFETY; DRUG SCREENING; DRUG TOLERABILITY; FECES ANALYSIS; FEMALE; FLATULENCE; GASTROINTESTINAL TRANSIT; HUMAN; HUMAN EXPERIMENT; INTESTINE FLORA; KIDNEY FUNCTION; LACTOBACILLUS; LACTOBACILLUS ACIDOPHILUS; LACTOBACILLUS FERMENTUM; LACTOBACILLUS GASSERI; LACTOBACILLUS PARACASEI; LIVER FUNCTION; MALE; NONHUMAN; NORMAL HUMAN; NUCLEOTIDE SEQUENCE; REAL TIME POLYMERASE CHAIN REACTION;

EID: 79954599460     PISSN: 18762883     EISSN: 18762891     Source Type: Journal    
DOI: 10.3920/BM2010.0023     Document Type: Article

References (49)

1. Ahlroos, T. and Tynkkynen, S., 2009. Quantitative strain-specific detection of Lactobacillus rhamnosus GG in human faecal samples by real-time PCR. Journal of Applied Microbiology 106: 506-514.
2. Alander, M., Satokari, R., Korpela, R., Saxelin, M., Vilpponen-Salmela, T., Mattila-Sandholm, T. and Von Wright, A., 1999. Persistence of colonization of human colonic mucosa by a probiotic strain, Lactobacillus rhamnosus GG, after oral consumption. Applied and Environmental Microbiology 65: 351-354.
3. Annuk, H., Shchepetova, J., Kullisaar, T., Songisepp, E., Zilmer, M. and Mikelsaar, M., 2003. Characterization of intestinal lactobacilli as putative probiotic candidates. Journal of Applied Microbiology 94: 403-412.
4. Anonymous, 2004. Method manual, Cobas Integra 400/700/800. Version 2. Roche Diagnostics GmbH, Mannheim, Germany.
5. Bartosch, S., Woodmansey, E.J., Paterson, J.C., McMurdo, M.E. and Macfarlane, G.T., 2005. Microbiological effects of consuming a synbiotic containing Bifidobacterium bifidum, Bifidobacterium lactis, and oligofructose in elderly persons, determined by real-time polymerase chain reaction and counting of viable bacteria. Clinical Infectious Diseases 40: 28-37.
6. Brigidi, P., Vitali, B., Swennen, E., Altomare, L., Rossi, M. and Matteuzzi, D., 2000. Specific detection of Bifidobacterium strains in a pharmaceutical probiotic product and in human feces by polymerase chain reaction. Systematic and Applied Microbiology 23: 391-399.
7. Cenciarini-Borde, C., Courtois, S. and La Scola, B., 2009. Nucleic acids as viability markers for bacteria detection using molecular tools. Future Microbiology 4: 45-64.
8. Detmer, A. and Glenting, J., 2006. Live bacterial vaccines - a review and identification of potential hazards. Microbial Cell Factories 5: 23.
9. Dommels, Y.E., Kemperman, R.A., Zebregs, Y.E., Draaisma, R.B., Jol, A., Wolvers, D.A., Vaughan, E.E. and Albers, R., 2009. Survival of Lactobacillus reuteri DSM 17938 and Lactobacillus rhamnosus GG in the human gastrointestinal tract with daily consumption of a low-fat probiotic spread. Applied and Environmental Microbiology 75: 6198-6204.
10. Food and Agriculture Organization of the United Nations/World Health Organization (FAO/WHO), 2001. Health and nutritional properties of probiotics in food including powder milk with live lactic acid bacteria. Report of a joint FAO/WHO expert consultation on evaluation of health and nutritional properties of probiotics in food including powder milk with live lactic acid bacteria. Córdoba, Argentina. Available at: http://www.who.int/foodsafety/publications/fs_management/en/probiotics.pdf.
11. Food and Agriculture Organization of the United Nations/World health Organization (FAO/WHO), 2002. Guidelines for the evaluation of probiotics in food. Available at: http://www.who.int/foodsafety/fs_management/en/probiotic_guidelines.pdf.
12. Frias, R., Ouwehand, A., Spillmann, T., Vankerckhoven, V., Hewicker-Trautwein, M., Salminen, S. and Gueimonde, M., 2009. Effect of clinical and probiotic Lactobacillus rhamnosus strains on intestinal permeability and bacterial translocation in healthy and colitic rats. Food Research International 42: 636-640.
13. Haarman, M. and Knol, J., 2006. Quantitative real-time PCR analysis of fecal Lactobacillus species in infants receiving a prebiotic infant formula. Applied and Environmental Microbiology 72: 2359-2365.
14. Hayford, A.E., Petersen, A., Vogensen, F.K. and Jakobsen, M., 1999. Use of conserved randomly amplified polymorphic DNA (RAPD) fragments and RAPD pattern for characterization of Lactobacillus fermentum in Ghanaian fermented maize dough. Applied and Environmental Microbiology 65: 3213-3221.
15. th ed. Roche Diagnostics GmbH, Mannheim, Germany.
16. Heilig, H.G., Zoetendal, E.G., Vaughan, E.E., Marteau, P., Akkermans, A.D. and De Vos, W.M., 2002. Molecular diversity of Lactobacillus spp. and other lactic acid bacteria in the human intestine as determined by specific amplification of 16S ribosomal DNA. Applied and Environmental Microbiology 68: 114-123.
17. Hütt, P., Shchepetova, J., Loivukene, K., Kullisaar, T. and Mikelsaar, M., 2006. Antagonistic activity of probiotic lactobacilli and bifidobacteria against entero-and uropathogens. Journal of Applied Microbiology 100: 1324-1332.
18. Huys, G., Vancanneyt, M., D'Haene, K., Vankerckhoven, V., Goossens, H. and Swings, J., 2006. Accuracy of species identity of commercial bacterial cultures intended for probiotic or nutritional use. Research in Microbiology 157: 803-810.
19. Jacobsen, C.N., Rosenfeldt, Nielsen, V., Hayford, A.E., Moller, P.L., Michaelsen, K.F., Paerregaard, A., Sandstrom, B., Tvede, M. and Jakobsen, M., 1999. Screening of probiotic activities of forty-seven strains of Lactobacillus spp. by in vitro techniques and evaluation of the colonization ability of five selected strains in humans. Applied and Environmental Microbiology 65: 4949-4956.
20. Kõll, P., Mändar, R., Smidt, I., Hütt, P., Truusalu, K., Mikelsaar, R.-H., Shchepetova, J., Krogh-Andersen, K., Marcotte, H., Hammarström, L. and Mikelsaar, M., 2010. Screening and evaluation of human intestinal lactobacilli for the development of novel gastrointestinal probiotics. Current Microbiology 61: 560-656.
21. Krüger, C., Hu, Y., Pan, Q., Marcotte, H., Hultberg, A., Delwar, D., Van Dalen, P.J., Pouwels, P.H., Leer, R.J., Kelly, C.G., Van Dollenweerd, C., Ma, J.K. and Hammarstrom, L., 2002. In situ delivery of passive immunity by lactobacilli producing single-chain antibodies. Nature Biotechnology 20: 702-706.
22. Kullisaar, T., Songisepp, E., Mikelsaar, M., Zilmer, K., Vihalemm, T. and Zilmer, M., 2003. Antioxidative probiotic fermented goats' milk decreases oxidative stress-mediated atherogenicity in human subjects. British Journal of Nutrition 90: 449-456.
23. Kutyavin, I.V., Afonina, I.A., Mills, A., Gorn, V.V., Lukhtanov, E.A., Belousov, E.S., Singer, M.J., Walburger, D.K., Lokhov, S.G., Gall, A.A., Dempcy, R., Reed, M.W., Meyer, R.B. and Hedgpeth, J., 2000. 3'-minor groove binder-DNA probes increase sequence specificity at PCR extension temperatures. Nucleic Acids Research 28: 655-661.
24. Lewis S., 2001. Reference ranges and normal values. In: Lewis, S., Bain, B. and Bates, I. (eds.) Dacie and Lewis practical haematology. Churchill Livingstone, London, UK.
25. Lidbeck, A. and Nord, C.E., 1993. Lactobacilli and the normal human anaerobic microflora. Clinical Infectious Diseases 16 Suppl. 4: S181-187.
26. Mättö, J., Fondén, R., Tolvanen, T., Von Wright, A., Vilpponen-Salmela, T., Satokari, R. and Saarela, M., 2006. Intestinal survival and persistence of probiotic Lactobacillus and Bifidobacterium strains administered in triple-strain yoghurt. International Dairy Journal 16: 1174-1180.
27. Mikelsaar, M., Annuk, H., Shchepetova, J., Mändar, R., Sepp, E. and Björksten, B., 2002. Intestinal lactobacilli of Estonian and Swedish children. Microbial Ecology in Health and Disease 14: 75-80.
28. Mikelsaar, M. and Zilmer, M., 2009. Lactobacillus fermentum ME-3 - an antimicrobial and antioxidative probiotic. Microbial Ecology in Health and Disease 21: 1-27.
29. Pant, N., Hultberg, A., Zhao, Y., Svensson, L., Pan-Hammarstrom, Q., Johansen, K., Pouwels, P.H., Ruggeri, F.M., Hermans, P., Frenken, L., Boren, T., Marcotte, H. and Hammarstrom, L., 2006. Lactobacilli expressing variable domain of llama heavy-chain antibody fragments (lactobodies) confer protection against rotavirus-induced diarrhea. Journal of Infectious Diseases 194: 1580-1588.
30. Pavan, S., Desreumaux, P. and Mercenier, A., 2003. Use of mouse models to evaluate the persistence, safety, and immune modulation capacities of lactic acid bacteria. Clinical and Diagnostic Laboratory Immunology 10: 696-701.
31. Reid, G., Anukam, K. and Koyama, T., 2008. Probiotic products in Canada with clinical evidence: what can gastroenterologists recommend? Canadian Journal of Gastroenterology 22: 169-175.
32. Saarela, M., Mogensen, G., Fonden, R., Matto, J. and Mattila-Sandholm, T., 2000. Probiotic bacteria: safety, functional and technological properties. Journal of Biotechnology 84: 197-215.
33. Saxelin, M., Pessi, T. and Salminen, S., 1995. Fecal recovery following oral administration of Lactobacillus strain GG (ATCC 53103) in gelatine capsules to healthy volunteers. International Journal of Food Microbiology 25: 199-203.
34. Seegers, J.F., 2002. Lactobacilli as live vaccine delivery vectors: progress and prospects. Trends in Biotechnology 20: 508-515.
35. Sepp, E., Julge, K., Vasar, M., Naaber, P., Bjorksten, B. and Mikelsaar, M., 1997. Intestinal microflora of Estonian and Swedish infants. Acta Paediatrica 86: 956-961.
36. Snydman, D.R., 2008. The safety of probiotics. Clinical Infectious Diseases 46 Suppl. 2: S104-111; discussion S144-151.
37. Songisepp, E., Kals, J., Kullisaar, T., Mandar, R., Hutt, P., Zilmer, M. and Mikelsaar, M., 2005. Evaluation of the functional efficacy of an antioxidative probiotic in healthy volunteers. Nutrition Journal 4: 22.
38. Stecher, B. and Hardt, W.D., 2008. The role of microbiota in infectious disease. Trends in Microbiology 16: 107-114.
39. Strahinic, I., Busarcevic, M., Pavlica, D., Milasin, J., Golic, N. and Topisirovic, L., 2007. Molecular and biochemical characterizations of human oral lactobacilli as putative probiotic candidates. Oral Microbiology and Immunology 22: 111-117.
40. Svedlund, J., Sjodin, I. and Dotevall, G., 1988. GSRS - a clinical rating scale for gastrointestinal symptoms in patients with irritable bowel syndrome and peptic ulcer disease. Digestive Diseases and Sciences 33: 129-134.
41. Timmerman, H.M., Niers, L.E., Ridwan, B.U., Koning, C.J., Mulder, L., Akkermans, L.M., Rombouts, F.M. and Rijkers, G.T., 2007. Design of a multispecies probiotic mixture to prevent infectious complications in critically ill patients. Clinical Nutrition 26: 450-459.
42. Torriani, S., Zapparoli, G. and Dellaglio, F. 1999. Use of PCR-based methods for rapid differentiation of Lactobacillus delbrueckii subsp. bulgaricus and L. delbrueckii subsp. lactis. Applied and Environmental Microbiology 65: 4351-4356.
43. Vankerckhoven, V., Huys, G., Vancanneyt, C.V., Vael, C., Klare, I., Romond, M.-B., Entenza, J.M., Moreillon, P., Wind, R.D., Knol, J., Wiertz, E., Pot, B., Vaughan, E.E., Kahlmeter, G. and Goossens, H., 2008. Biosafety assessment of probiotics used for human consumption: recommendations from the EU-PROSAFE project. Trends in Food Science & Technology 19: 102-144.
44. Verdenelli, M.C., Ghelfi, F., Silvi, S., Orpianesi, C., Cecchini, C. and Cresci, A., 2009. Probiotic properties of Lactobacillus rhamnosus and Lactobacillus paracasei isolated from human faeces. European Journal of Nutrition 48: 355-363.
45. Vesterlund, S., Vankerckhoven, V., Saxelin, M., Goossens, H., Salminen, S. and Ouwehand, A.C., 2007. Safety assessment of Lactobacillus strains: presence of putative risk factors in faecal, blood and probiotic isolates. International Journal of Food Microbiology 116: 325-331.
46. Vlieger, A.M., Robroch, A., Van Buuren, S., Kiers, J., Rijkers, G., Benninga, M.A. and Te Biesebeke, R., 2009. Tolerance and safety of Lactobacillus paracasei ssp. paracasei in combination with Bifidobacterium animalis ssp. lactis in a prebiotic-containing infant formula: a randomised controlled trial. British Journal of Nutrition 102: 869-875.
47. Walter, J., 2008. Ecological role of lactobacilli in the gastrointestinal tract: implications for fundamental and biomedical research. Applied and Environmental Microbiology 74: 4985-4996.
48. Zhao, Y., Liu, Z., Yu, S., Wen, S., Hammarström, L. and Rabbani, H., 2009. Construction of a high efficiency PCR products cloning T Vector using pGEM-5zf (+). Avicenna Journal of Medical Biotechnology 1: 37-39.
49. Zhong, W., Millsap, K., Bialkowska-Hobrzanska, H. and Reid, G., 1998. Differentiation of Lactobacillus species by molecular typing. Applied and Environmental Microbiology 64: 2418-2423.