In vivo imaging of human and mouse skin with a handheld dual-axis confocal fluorescence microscope

Journal of Investigative Dermatology

Volumn 131, Issue 5, 2011, Pages 1061-1066

  Ra, Hyejun ^a,b   Piyawattanametha, Wibool ^a,c,d   Gonzalez Gonzalez, Emilio ^a   Mandella, Michael J ^a,b   Kino, Gordon S ^b   Solgaard, Olav ^b   Leake, Devin ^e   Kaspar, Roger L ^a,f   Oro, Anthony ^g   Contag, Christopher H ^a  

^a STANFORD UNIVERSITY   (United States)

^b STANFORD UNIVERSITY   (United States)

^c NATIONAL ELECTRONICS AND COMPUTER TECHNOLOGY CENTER   (Thailand)

^d CHULALONGKORN UNIVERSITY   (Thailand)

^e Thermo Fisher Scientific   (United States)

^f TransDerm   (United States)

^g STANFORD UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CONTRAST MEDIUM; DYE; INDOCYANINE GREEN; NEAR INFRARED DYE; SMALL INTERFERING RNA; TD 101; UNCLASSIFIED DRUG;

ANIMAL TISSUE; ARTICLE; CALLUS; COMPARATIVE STUDY; CONFOCAL MICROSCOPY; CONGENITAL PACHYONYCHIA; CONTROLLED STUDY; DRUG CONJUGATION; DRUG DISTRIBUTION; DRUG EFFICACY; DRUG LABELING; DRUG TARGETING; EPIDERMIS; FLUORESCENCE IMAGING; FLUORESCENCE MICROSCOPE; FLUORESCENCE MICROSCOPY; GENE MUTATION; HANDHELD DUAL AXIS CONFOCAL FLUORESCENCE MICROSCOPE; HISTOLOGY; HUMAN; HUMAN TISSUE; IN VIVO STUDY; KERATINOCYTE; MOLECULAR THERAPY; MOUSE; NONHUMAN; PRIORITY JOURNAL; SKIN PENETRATION; STRATUM CORNEUM; THREE DIMENSIONAL IMAGING;

EID: 79954586044     PISSN: 0022202X     EISSN: 15231747     Source Type: Journal    
DOI: 10.1038/jid.2010.401     Document Type: Article

References (19)

1. Gonzalez-Gonzalez E, Ra H, Hickerson RP et al. (2009) siRNA silencing of keratinocyte-specific GFP expression in a transgenic mouse skin model. Gene Therapy 16:963-72
2. Gonzalez-Gonzalez E, Ra H, Spitler R et al. (2010a) Increased interstitial pressure improves nucleic acid delivery to skin enabling a comparative analysis of constitutive promoters. Gene Therapy 17:1270
3. Gonzalez-Gonzalez E, Speaker TJ, Hickerson RP et al. (2010b) Silencing of reporter gene expression in skin using siRNAs and expression of plasmid DNA delivered by a soluble protrusion array device (PAD). Mol Ther 18:1667
4. Hickerson RP, Leachman SA, Pho LN et al. (2011) Development of quantitative molecular clinical end points for siRNA clinical trials. J Invest Dermatol 131:1029-36
5. Hickerson RP, Smith FJ, Reeves RE et al. (2008) Single-nucleotide- specific siRNA targeting in a dominant-negative skin model. J Invest Dermatol 128:594-605 (Pubitemid 351252662)
6. Leachman SA, Hickerson RP, Hull PR et al. (2008) Therapeutic siRNAs for dominant genetic skin disorders including pachyonychia congenita. J Dermatol Sci 51:151-7
7. Leachman SA, Hickerson RP, Schwartz ME et al. (2010) First-in-human mutation-targeted siRNA phase Ib trial of an inherited skin disorder. Mol Ther 18:442-6
8. Leachman SA, Kaspar RL, Fleckman P et al. (2005) Clinical and pathological features of pachyonychia congenita. J Investig Dermatol Symp Proc 10:3-17
9. Marshall MV, Draney D, Sevick-Muraca EM et al. (2010) Single-dose intravenous toxicity study of IRDye 800CW in Sprague-Dawley rats. Mol Imaging Biol 12:583
10. McLean WH, Lane EB (1995) Intermediate filaments in disease. Curr Opin Cell Biol 7:118-25
11. Piyawattanametha W, Ra H, Mandella MJ et al. (2009) 3-D near-infrared fluorescence imaging using an MEMS-based miniature dual-axis confocal microscope. IEEEJ Sel Top Quantum Electron 15:1344-50
12. Ra H, Gonzalez-Gonzalez E, Smith BR et al. (2010) Assessing delivery and quantifying efficacy of small interfering ribonucleic acid therapeutics in the skin using a dual-axis confocal microscope. J Biomed Opt 15:036027
13. Ra H, Piyawattanametha W, Mandella MJ et al. (2008) Three-dimensional in vivo imaging by a handheld dual-axes confocal microscope. Opt Express 16:7224-32 (Pubitemid 351697408)
14. Ra H, Piyawattanametha W, Taguchi Y et al. (2007) Two-dimensional MEMS scanner for dual-axes confocal microscopy. J Microelectromech Syst 16:969-76 (Pubitemid 47278838)
15. Smith FJ, Hickerson RP, Sayers JM et al. (2008) Development of therapeutic siRNAs for pachyonychia congenita. J Invest Dermatol 128:50-8 (Pubitemid 350249391)
16. Smith FJ, Liao H, Cassidy AJ et al. (2005) The genetic basis of pachyonychia congenita. J Investig Dermatol Symp Proc 10:21-30
17. Takanashi M, Oikawa K, Sudo K et al. (2009) Therapeutic silencing of an endogenous gene by siRNA cream in an arthritis model mouse. Gene Therapy 16:982-9
18. Wang TD, Mandella MJ, Contag CH et al. (2003) Dual-axis confocal microscope for high-resolution in vivo imaging. Opt Lett28:414-6
19. White PJ, Gray AC, Fogarty RD et al. (2002) C-5 propyne-modified oligonucleotides penetrate the epidermis in psoriatic and not normal human skin after topical application. J Invest Dermatol 118:1003-7 (Pubitemid 34639179)