In situ detection of active transglutaminases for keratinocyte type (tgase 1) and tissue type (tgase 2) using fluorescence-labeled highly reactive substrate peptides

Journal of Histochemistry and Cytochemistry

Volumn 59, Issue 2, 2011, Pages 180-187

  Itoh, Miho ^a   Kawamoto, Tadafumi ^b   Tatsukawa, Hideki ^c   Kojima, Soichi ^c   Yamanishi, Kiyofumi ^d   Hitomi, Kiyotaka ^a  

^a NAGOYA UNIVERSITY   (Japan)

^b TSURUMI UNIVERSITY   (Japan)

^c RIKEN   (Japan)

^d HYOGO COLLEGE OF MEDICINE   (Japan)

Author keywords

Calcium; Connective tissue; Epithelium; Transglutaminase

Indexed keywords

PROTEIN GLUTAMINE GAMMA GLUTAMYLTRANSFERASE; PROTEIN GLUTAMINE GAMMA GLUTAMYLTRANSFERASE 2; TRANSGLUTAMINASE 1; UNCLASSIFIED DRUG; FLUORESCENT DYE; GUANINE NUCLEOTIDE BINDING PROTEIN; ISOENZYME; OLIGOPEPTIDE;

AMINO ACID SEQUENCE; ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTICLE; CONTROLLED STUDY; ENZYME ASSAY; FLUORESCENCE ANALYSIS; FLUORESCENCE IMAGING; INTESTINE MUCOSA; KERATINOCYTE; MALE; MOUSE; NONHUMAN; PRIORITY JOURNAL; TISSUE DISTRIBUTION; TOOTH DEVELOPMENT; ANIMAL; ANTIBODY SPECIFICITY; CHEMISTRY; ENZYME SPECIFICITY; FROZEN SECTION; METABOLISM; METHODOLOGY; TIME;

MAMMALIA;

AMINO ACID SEQUENCE; ANIMALS; ENZYME ASSAYS; FLUORESCENT DYES; FROZEN SECTIONS; GTP-BINDING PROTEINS; ISOENZYMES; MALE; MICE; OLIGOPEPTIDES; ORGAN SPECIFICITY; SUBSTRATE SPECIFICITY; TIME FACTORS; TRANSGLUTAMINASES;

EID: 79953780725     PISSN: 00221554     EISSN: None     Source Type: Journal    
DOI: 10.1369/jhc.2010.957225     Document Type: Article

References (35)

1. Aeschlimann D, Koeller MK, Allen-Hoffmann BL, Mosher DF. 1998. Isolation of a cDNA encoding a novel member of the transglutaminase gene family from human keratinocytes: detection and identification of transglutaminase gene products based on reverse transcription-polymerase chain reaction with degenerate primers. J Biol Chem. 273:3452-3460.
2. Akiyama M, Sakai K, Yanagi T, Fukushima S, Ihn H, Hitomi K, Shimizu H. 2010. Transglutaminase 1 preferred substrate peptide K5 is an efficient tool in diagnosis of lamellar ichthyosis. Am J Pathol. 176:1592-1599.
3. Beninati S, Piacentini M. 2004. The transglutaminase family: an overview. Amino Acids. 26:367-372.
4. Candi E, Schmidt R, Melino G. 2005. The cornified envelope: a model of cell death in the skin. Nat Rev Mol Cell Biol. 6:328-340.
5. Eckert RL, Sturniolo MT, Broome AM, Ruse M, Rorke EA. 2005. Transglutaminase function in epidermis. J Invest Dermatol. 124:481-492.
6. Esposito C, Pucci P, Amoresano A, Marino G, Cozzolino A, Porta R. 1996. Transglutaminase from rat coagulating gland secretion: post-translational modification and activation by phosphatidic acids. J Biol Chem. 271:27416-27423.
7. Fesus L, Piacentini M. 2002. Transglutaminase 2: an enigmatic enzyme with diverse functions. Trends Biochem Sci. 27: 534-539.
8. Furutani Y, Kato A, Notoya M, Ghoneim MA, Hirose S. 2001. A simple assay and histochemical localization of transglutaminase activity using a derivative of green fluorescent protein as substrate. J Histochem Cytochem. 49:247-258.
9. Grenard P, Bates MK, Aeschlimann D. 2001. Evolution of transglutaminase genes: identification of a transglutaminase gene cluster on human chromosome 15q15. J Biol Chem. 276:33066-33078.
10. Griffin M, Casadio R, Bergamini CM. 2002. Transglutaminases: nature's biological glues. Biochem J. 368:377-396.
11. Hiiragi T, Sasaki H, Nagafuchi A, Sabe H, Shen SC, Matsuki M, Yamanishi K, Tsukita S. 1999. Transglutaminase type 1 and its cross-linking activity are concentrated at adherens junctions in simple epithelial cells. J Biol Chem. 274:34148-34154.
12. Hitomi K. 2005. Transglutaminase in skin epidermis. Eur J Dermatol. 15:313-319.
13. Hitomi K, Kitamura M, Perez Alea M, Ceylon I, Thomas V, El Alaoui S. 2009. A specific colorimetric assay for measuring transglutaminase 1 and factor XIII activities. Anal Biochem. 394:281-283.
14. Hitomi K, Kitamura M, Sugimura Y. 2009. Preferred substrate sequences for transglutaminase 2: screening using a phagedisplayed peptide library. Amino Acids. 36:619-624.
15. Huber M, Rettler I, Bernasconi K, Frenk E, Lavrijsen SPM, Ponec M, Bon A, Lautenschlager S, Schorderet DF, Hohl D. 1995. Mutations of keratinocyte transglutaminase in lamellar ichthyosis. Science. 267:525-528.
16. Ichinose A. 2001. Physiopathology and regulation of factor XIII. Thromb Haemost. 86:57-65.
17. Jeon JH, Kim IG. 2006. Role of protein modification mediated by transglutaminase 2 in human viral diseases. Front Biosci. 11:221-231.
18. Kalinin AE, Kajava AV, Steinert PM. 2002. Epithelial barrier function: assembly and structural features of the cornified cell envelop. BioEssays. 24:789-800.
19. Kawamoto T. 2003. Use of a new adhesive film for the preparation of multi-purpose fresh-frozen sections from hard tissues, wholeanimals, insects and plants. Arch Histrol Cytol. 66:123-143.
20. Kawamoto T, Shimizu M. 2000. A method for preparing 2- to 50-μm-thick fresh frozen section of large samples and undecalcified hard tissues. Histochem Cell Biol. 113:331-339.
21. Lorand L, Graham RM. 2003. Transglutaminases: crosslinking enzymes with pleiotropic functions. Nat Rev Mol Cell Biol. 4:140-156.
22. Matsuki M, Yamashita F, Ishida-Yamamoto A, Yamada K, Kinoshita C, Fushiki S, Ueda E, Morishima Y, Tabata K, Yasuno H, Hashida M, Iizuka H, Ikawa M, Okabe M, Kondoh G, Kinoshita T, Takeda J, Yamanishi K. 1998. Defective stratum corneum and early neonatal death in mice lacking the gene for transglutaminase 1. Proc Natl Acad Sci U S A. 95:1044-1049.
23. Mehta K. 2009. Biological and therapeutic significance of tissue transglutaminase in pancreatic cancer. Amino Acids. 36: 709-716.
24. Mehta K, Fok JY, Mangala LS. 2006. Tissue transglutaminase: from biological glue to cell survival cues. Front Biosci. 11:173-185.
25. Nanda N, Iismaa SE, Owens WA, Husain A, Mackay F, Graham RM. 2001. Targeted inactivation of Gh/tissue transglutaminase II. J Biol Chem. 276:20673-20678.
26. Oji V, Oji ME, Adamini N, Walker T, Aufenvenne K, Raghunath M, Traupe H. 2006. Plasminogen activator inhibitor-2 is expressed in different types of congenital ichthyosis: in vivo evidence for its cross-linking into the cornified cell envelope by transglutaminase-1. Br J Dermatol. 154:860-867.
27. Perez Alea M, Kitamura M, Martin G, Thomas V, Hitomi K, El Alaoui S. 2009. Development of an isozyme-specific colorimetric assay for tissue transglutaminase 2 cross-linking activity. Anal Biochem. 389:150-156.
28. Ruan Q, Johnson GV. 2007. Transglutaminase 2 in neurogenerative disease. Front Biosci. 12:891-904.
29. Sarang Z, Toth B, Balajthy Z, Koroskenyi, Garabuczi E, Fesus L, Szondy Z. 2009. Some lessons from the tissue transglutaminase knockout mouse. Amino Acids. 36:625-631.
30. Sollid LM. 2002. Coeliac disease: dissecting a complex inflammatory disorder. Nat Rev Immunol. 2:647-655.
31. Sugimura Y, Hosono M, Kitamura M, Tsuda T, Yamanishi K, Maki M, Hitomi K. 2008. Identification of preferred substrate sequences for transglutaminase 1: development of a novel peptide that can efficiently detect cross-linking enzyme activity in the skin. FEBS J. 275:5667-5677.
32. Sugimura Y, Hosono M, Wada F, Yoshimura T, Maki M, Hitomi K. 2006. Screening for the preferred substrate sequence of transglutaminase using a phage-displayed peptide library: identification of peptide substrates for TGase 2 and factor XIIIa. J Biol Chem. 281:17699-17706.
33. Sugimura Y, Ueda H, Maki M, Hitomi K. 2007. Novel site-specific immobilization of functional protein using a preferred substrate sequence for transglutaminase 2. J Biotechnol. 131:121-127.
34. Tatsukawa H, Fukaya Y, Frampton G, Martinez-Fuentes A, Suzuki K, Kuo TF, Nagatsuma K, Shimokado K, Okuno M, Wu J, Iismaa S, Matsuura T, Tsukamoto H, Zern MA, Graham RM, Kojima S. 2009. Role of transglutaminase 2 in liver injury via cross-linking and silencing of transcription factor Sp1. Gastroenterology. 136:1783-1795.
35. Van Nooden CJF. 2010. Imaging enzymes at work: metabolic mapping by enzyme histochemistry. J Histochem Cytochem. 58:481-497.