A specific colorimetric assay for measuring transglutaminase 1 and factor XIII activities

Analytical Biochemistry

Volumn 394, Issue 2, 2009, Pages 281-283

  Hitomi, Kiyotaka ^a   Kitamura, Miyako ^a   Alea, Mileidys Perez ^b   Ceylan, Ismail ^b   Thomas, Vincent ^b   El Alaoui, Saïd ^b  

^a NAGOYA UNIVERSITY   (Japan)

^b CovalAb SARL ^*  (France)

Author keywords

[No Author keywords available]

Indexed keywords

SUBSTRATES;

COLORIMETRIC ASSAYS; ENZYMATIC ACTIVITIES; PEPTIDE LIBRARY; PRIMARY AMINES; TISSUE DISTRIBUTIONS; TRANSGLUTAMINASES;

AMINES;

BLOOD CLOTTING FACTOR 13; PROTEIN GLUTAMINE GAMMA GLUTAMYLTRANSFERASE; PROTEIN GLUTAMINE GAMMA GLUTAMYLTRANSFERASE 2;

ARTICLE; COLORIMETRY; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME ASSAY; ENZYME SUBSTRATE; MOLECULAR LIBRARY; PHAGE DISPLAY; PRIORITY JOURNAL; TISSUE DISTRIBUTION;

EID: 69949094931     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2009.07.039     Document Type: Article

References (11)

1. Griffin M., Casadio R., and Bergamini C.M. Transglutaminases: nature's biological glues. Biochem. J. 368 (2002) 377-396
2. Lorand L., and Graham R.M. Transglutaminases: crosslinking enzymes with pleiotropic functions. Nat. Rev. Mol. Cell Biol. 4 (2003) 140-156
3. Sugimura Y., Hosono M., Wada F., Yoshimura T., Maki M., and Hitomi K. Screening for the preferred substrate sequence of transglutaminase using a phage-displayed peptide library: identification of peptide substrates for TGase 2 and factor XIIIA. J. Biol. Chem. 281 (2006) 17699-17706
4. Hitomi K., Kitamura M., and Sugimura Y. Preferred substrate sequences for transglutaminase 2: screening using a phage-displayed peptide library. Amino Acids 36 (2009) 619-624
5. Sugimura Y., Hosono M., Kitamura M., Yamanishi K., Tsuda T., Maki M., and Hitomi K. Identification of preferred substrate sequences for transglutaminase 1: development of a novel peptide that can efficiently detect cross-linking enzyme activity in the skin. FEBS J. 275 (2008) 5667-5677
6. Alea M.P., Kitamura M., Martin G., Thomas V., Hitomi K., and El Alaoui S. Development of a highly sensitive and specific colorimetric assay for the measurement of TGase 2 cross-linking activity. Anal. Biochem. 389 (2009) 150-156
7. Hitomi K., Yamagiwa Y., Ikura K., Yamanishi K., and Maki M. Characterization of human recombinant transglutaminase 1 purified from baculovirus-infected insect cells. Biosci. Biotechnol. Biochem. 64 (2000) 2128-2137
8. Thomas V., El Alaoui S., Massignon D., Clement S., Simonet F., and Quash G. Development and evaluation of a modified colorimetric solid-phase microassay for measuring the activity of cellular and plasma (Factor XIII) transglutaminases. Biotechnol. Appl. Biochem. 43 (2006) 171-179
9. Slaughter T.F., Achyuthan K.E., Lai T.S., and Greenberg C.S. A microtiter plate transglutaminase assay utilizing 5-(biotinamido)pentylamine as substrate. Anal. Biochem. 205 (1992) 166-171
10. Trigwell S.M., Lynch P.T., Griffin M., Hargreave A.J., and Bonner P.L. An improved colorimetric assay for the measurement of transglutaminase (type II) ε-(γ-glutamyl) lysine cross-linking activity. Anal. Biochem. 330 (2004) 164-166
11. Madi A., Karpati L., Kovacs A., Muszbek L., and Fesus L. High-throughput scintillation proximity assay for transglutaminase activity measurement. Anal. Biochem. 343 (2005) 256-262