The role of the disulfide bridge in the stability and structural integrity of ovalbumin evaluated by site-directed mutagenesis

Bioscience, Biotechnology and Biochemistry

Volumn 75, Issue 3, 2011, Pages 544-549

  Ishimaru, Takayuki ^a   Ito, Kazunari ^b   Tanaka, Miho ^a   Tanaka, Syunpei ^a   Matsudomi, Naotoshi ^a,c  

^a YAMAGUCHI UNIVERSITY   (Japan)

^b INDUSTRIAL TECHNOLOGY CENTER OF OKAYAMA PREFECTURE   (Japan)

^c Ube Frontier University   (Japan)

Author keywords

Conformational stability; Disulfide bridge; Ovalbumin; S ovalbumin

Indexed keywords

ALKALINE TREATMENT; CIRCULAR DICHROISM; CONFORMATIONAL STABILITIES; DISULFIDE BRIDGE; ELASTASE; NON-NATIVE; OVALBUMIN; OVALBUMINS; SITE DIRECTED MUTAGENESIS; THERMAL DENATURATIONS; TRYPTOPHAN FLUORESCENCE; WILD TYPES;

AMINO ACIDS; COVALENT BONDS; DICHROISM; PYROLYSIS; STRUCTURAL INTEGRITY;

STABILITY;

ALANINE; DISULFIDE; OVALBUMIN; PANCREATIC ELASTASE; PEPTIDE FRAGMENT; RECOMBINANT PROTEIN; SERINE;

ANIMAL; ARTICLE; CHEMICAL STRUCTURE; CHEMISTRY; CHICKEN; CIRCULAR DICHROISM; ESCHERICHIA COLI; GENE EXPRESSION; GENETICS; METABOLISM; MOLECULAR CLONING; MUTATION; PH; PROTEIN CONFORMATION; PROTEIN DENATURATION; PROTEIN STABILITY; SITE DIRECTED MUTAGENESIS; SPECTROFLUOROMETRY; TEMPERATURE;

ALANINE; ANIMALS; CHICKENS; CIRCULAR DICHROISM; CLONING, MOLECULAR; DISULFIDES; ESCHERICHIA COLI; GENE EXPRESSION; HYDROGEN-ION CONCENTRATION; MODELS, MOLECULAR; MUTAGENESIS, SITE-DIRECTED; MUTATION; OVALBUMIN; PANCREATIC ELASTASE; PEPTIDE FRAGMENTS; PROTEIN CONFORMATION; PROTEIN DENATURATION; PROTEIN STABILITY; RECOMBINANT PROTEINS; SERINE; SPECTROMETRY, FLUORESCENCE; TEMPERATURE;

EID: 79953778309     PISSN: 09168451     EISSN: 13476947     Source Type: Journal    
DOI: 10.1271/bbb.100772     Document Type: Article

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